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Rabbit anti Monkey IgG Fab Secondary Antibody

Scan QR to view Datasheet Catalog #    MBS571335 Rabbit anti Monkey IgG Fab secondary antibody
Unit / Price
10 mg  /  $475 +1 FREE 8GB USB
 
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 Product Name   

Rabbit anti Monkey IgG Fab, Secondary Antibody

★Popular Item★
 Also Known As   

Rabbit anti Monkey IgG Fab

 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Monkey
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 Specificity    Purified IgG fraction of polyclonal Rabbit antiSerum to Monkey Fab of IgG
 Purity/Purification    Hyperimmune antisera with strong precipitating activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
 Form/Format    Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
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 Immunogen    Purified Fab from normal IgG isolated from pooled Rhesus monkey serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
 Absorption    Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies reacting with other subunits of the immunoglobulin or reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of foreign protein or immune complexes in the antiserum.
 Reconstitution    It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C.
 Preservative    No preservative added, as it may interfere with the antibody activity. No foreign protein added.
 Identity and Specifity    The antiserum is reacting with the Fab subunit of intact IgG, IgA and IgM and other Ig classes of both light chain types, with their Fab or F(ab)2 subunits and with free light chains of kappa and lambda type as tested in immunoelectrophoresis and double radial immunodiffusion.
 Physochemical characteristics    IgG protein concentration 10 mg/mL.No foreign proteins added.
 Antibody titre    Precipitin tire not less than 1:64 when tested againsnt normal monkey serum in agar block titration.
 Cross-reactivity    Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however in immunoelectrophoresis a reaction with Human has been observed.
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 Preparation and Storage    The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4C; prolonged storage at or below -20C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.


Lyophilized at 4C :at least 10 years.
Reconstituted at or below -20C:3-5 years..
Reconstituted at 4C:7 days.
 Other Notes    Small volumes of Rabbit anti Monkey IgG Fab secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for Rabbit anti Monkey IgG Fab secondary antibody

   For indirect staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa and lambda type. In general this kind of products is not recommended as direct or indirect screening reagents for immunoglobulin isotypes on the surface of membranes of vital lymphoid cells. The presence of activity to the common Fab subunit may result in the staining of Ig bound to Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
 Product Categories/Family for Rabbit anti Monkey IgG Fab secondary antibody    Secondary Antibody
 Applications Tested/Suitable for Rabbit anti Monkey IgG Fab secondary antibody   

Indirect Immunofluorescence (IF), ELISA (EIA), Dot Blot (DB), Immunoblotting (IB)

 Application Notes for Rabbit anti Monkey IgG Fab secondary antibody    When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.

Typical working dilutions in histochemistry are usually between 1 :50 and 1 :500; in ELISA and comparable non-precipitating antibody-binding assays between 1: 1000 and 1 :5,000.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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