View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List Sheep anti Guinea Pig IgM (Fc specific) Secondary Antibody Catalog # MBS571459 Unit / Price 1 mL / $390 +1 FREE 8GB USB 5x1 mL / $1,580 +1 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Sheep anti Guinea Pig IgM (Fc specific), Secondary Antibody Popular Item Full Product Name Sheep anti Guinea Pig IgM (Fc specific) Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Polyclonal Host Sheep Species Reactivity Guinea Pig Specificity Precipitating polyclonal Sheep antiSerum to Guinea Pig IgM, Fc specific. Form/Format Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal sheep serum. No foreign proteins added. Source Highly purified normal IgM isolated from pooled guinea pig serum. Freunds complete adjuvant is used in the first step of the immunization procedure. Cross- reactivity The antiSerum does not cross-react with any other component of the Guinea Pig Ig system.Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail. Reconstitution Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Preparation and Storage The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day. Other Notes Small volumes of Sheep anti Guinea Pig IgM (Fc specific) secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for Sheep anti Guinea Pig IgM (Fc specific) secondary antibody The reactivity of the antiserum is restricted to the Fc part of the IgM molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal guinea pig serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgM. No precipitation reaction is obtained with purified IgG, IgA, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in guinea pig serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of guinea pig IgM from serum or plasma. Product Categories/Family for Sheep anti Guinea Pig IgM (Fc specific) secondary antibody Antisera Applications Tested/Suitable for Sheep anti Guinea Pig IgM (Fc specific) secondary antibody Immunoelectrophoresis (IEP) Application Notes for Sheep anti Guinea Pig IgM (Fc specific) secondary antibody Precipitation assays. In immunoelectrophoresis use 2 ul or equivalent against 120 ul antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ul in a 3 mm diameter center well and 2 ul serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP