View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List Sheep anti Human IgA (Fc specific) Secondary Antibody Catalog # MBS571902 Unit / Price 1 mL / $580 +1 FREE 8GB USB 5x1 mL / $2,440 +3 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Sheep anti Human IgA (Fc specific), Secondary Antibody Popular Item Full Product Name Sheep anti Human IgA (Fc specific), conjugated with TRITC Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Polyclonal Host Sheep Species Reactivity Human Specificity Tetramethylrhodamine isothiocyanate-conjugated F(ab')2 fragments of polyclonal Sheep IgG antibodies to Human IgA, Fc specific. Form/Format TRITC-coupled sheep F(abÕ)2 antibodies lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added. Product TRITC-coupled sheep F(ab)2 antibodies lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Reactivity Note Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this conjugate has not been tested in detail. Preparation and Storage The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Other Notes Small volumes of Sheep anti Human IgA (Fc specific) secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for Sheep anti Human IgA (Fc specific) secondary antibody The antiserum is reacting with to the Fc subunits of IgA. It does not react with other immunoglobulins or any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of IgA. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Product Categories/Family for Sheep anti Human IgA (Fc specific) secondary antibody Human immunoglobulins Applications Tested/Suitable for Sheep anti Human IgA (Fc specific) secondary antibody ELISA (EIA), Immunocytochemistry (ICC), Immunohistochemistry (IHC) (frozen), (In)direct Immunofluorescence (IF) TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP