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Mammal Tissue Protein Extraction Reagent Western Blot Related Reagent

Scan QR to view Datasheet Catalog #    MBS176732
Testing Data
Unit / Price
100 mL  /  $160 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Mammal Tissue Protein Extraction Reagent, Western Blot Related Reagent

★Popular Item★
 Also Known As   

Mammal Tissue Protein Extraction Reagent

 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Form/Format    Liquid-Solution
 Concentration    Recommended working concentration: 1ml of extraction reagent per 0.1g of tissue for tissue samples homogenization (lot specific)
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 Reagent Type    Universal tissue extraction buffer, detergent solution, cell lysis buffer
 Content    100 ml Mammal Tissue Protein Extraction Reagent
 Usage    Obtaining homogenous tissue cell lysates with soluble protein extracts
 Product Type    Ready to use
 pH    7.6, physiological
 Reactivity/severeness    Bland, minimal amount of detergent, detergent is dialyzable
 Enzymatic Activity    None; use additives to add function
 Additional components compatibility    Compatible with additives e.g. protease inhibitors (PMSF), reducing agents, chelating agents, salts
 Downstream methods compatibility    Compatible with standard protein detection methods; immunoassays; enzyme assays; common reporter assays
    
 Usage and Handling    1. Place the fresh tissue into pre-cooling normal saline and wash several times to clean the blood on the surface.
2. Weigh the tissue samples, cut into pieces before adding to a glass homogenizer.
3. Prepare tissue samples for homogenization in extraction reagent, using 1ml reagent per 0.1g of tissue. Reduce the amount of extraction reagent if need protein samples with higher concentration.
4. Add 100mmol/ml PMSF before using and dilute to 1m mol/ml.
5. Homogenize using a glass homogenizer until the tissue samples were lysed completely.
6. Increase the amount of extraction reagent if the samples were not lysed completely;
7. Centrifuge for 3-5 minutes at 10000-14000 g.
8. Get the supernatant solution, and move to the next steps.
    
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 Preparation and Storage    Store at 4C for frequent use, at -20C for at least one year
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of Mammal Tissue Protein Extraction Reagent western blot related reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for Mammal Tissue Protein Extraction Reagent western blot related reagent

   Description:
Mammal Tissue Protein Extraction Reagent is a ready-to-use Western blot related reagent solution used for efficient extraction of total soluble protein in nondenatured state from animal tissues by homogenization and cell lysis utilizing a dialyzable mild detergent at low concentration, for maximal protein solubilization and minimal interference with protein biological activity enabling direct use in downstream analysis.

Background:
Extraction of tissue proteins being an essential step in the process of protein sample preparation is critical to successful protein analysis. Successful tissue protein extraction requires efficient cell lysis and protein solubilization, while preserving protein integrity and immunoreactivity. The varying tissue composition in different tissue samples implies different approaches for efficient and homogenous tissue protein extraction. The Mammal Tissue Protein Extraction Reagent was designed for use with all tissues, independent of mechanical extraction methods. A mild nondenaturing and easily dialyzable detergent is added to the solution that aids in dissolving cell membranes and enhances solubilization of total protein without compromising function, and the neutral pH and salt concentration of the reagent enable more efficient extraction from cellular compartments yielding homogeneous lysates. The produced cell lysates are directly compatible with reporter gene expression assays (luciferase, beta-galactosidase, chloramphenicol acetyl transferase, CAT, alkaline phosphatase), protein kinase assays (PKA, PKC, tyrosine kinase), phosphatase assays (general phosphatases, tyrosine phosphatases), immunoassays ( Western blots, ELISAs, RIAs, immunoprecipitation), Coomassie-Blue and silver staining, protein purification procedures, electrophoresis, folding studies, chromatographic studies, DNA-protein interaction assays (gel-shift assays), and many other downstream applications.
 Product Categories/Family for Mammal Tissue Protein Extraction Reagent western blot related reagent    Western Blotting Related Reagent
 Application Notes for Mammal Tissue Protein Extraction Reagent western blot related reagent    Immunoassays: WB, ELISA, RIA, immunoprecipitation; reporter assays; protein kinase assays; protein purification; folding studies; chromatographic studies; DNA-protein interaction assays
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 Testing Data of Mammal Tissue Protein Extraction Reagent western blot related reagent    3. Prepare tissue samples for homogenization in extraction reagent, using 1ml reagent per 0.1g of tissue. Reduce the
Mammal Tissue Protein Extraction Reagent western blot related reagent Testing Data image
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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