View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Add To Compare List Micro BCA Protein Western Blot Related Reagent Catalog # MBS176722 Unit / Price DISCONTINUEDYou will be redirected to our replacement product Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Micro BCA Protein, Western Blot Related Reagent Popular Item Full Product Name Micro BCA Protein Assay Kit Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Preparation and Storage Store at 4 degree C (BSA Albumin Standard Ampules should be stored at -20 degree C) ISO Certification Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory. Other Notes Small volumes of Micro BCA Protein western blot related reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for Micro BCA Protein western blot related reagent The Micro BCA Protein Assay Kit is a detergent-compatible bicinchoninic acid formulation for the colorimetric detection and quantitation of total protein. An adaptation of the BCA Protein Assay Kit, the Micro BCA Kit has been optimized for use with dilute protein samples (0.5-20 ug/ml). The unique, patented method utilizes bicinchoninic acid (BCA) as the detection reagent for Cu+1, which is formed when Cu+2 is reduced by protein in an alkaline environment. A purple-colored reaction product is formed by the chelation of two molecules of BCA with one cuprous ion (Cu+1). This water-soluble complex exhibits a strong absorbance at 562 nm that is linear with increasing protein concentrations. The macromolecular structure of protein, the number of peptide bonds and the presence of four amino acids (cysteine, cystine, tryptophan and tyrosine) are reported to be responsible for color formation with BCA. Studies with di-, tri- and tetrapeptides suggest that the extent of color formation is caused by more than the mere sum of individual color-producing functional groups. He Micro BCA Protein Assay Kit uses concentrated reagents and a protocol that utilizes an extended incubation time at an elevated temperature (60 degree C, Test Tube Procedure only). The result is an extremely sensitive colorimetric protein assay in a test tube or microplate assay format. Product Categories/Family for Micro BCA Protein western blot related reagent Western Blotting Related Reagent Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP