View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List Tissue cDNA, First Strand Diseased cDNA Clone Catalog # MBS652445 Unit / Price 40 Tests / $1,420 +1 FREE 8GB USB 5x40 Tests / $6,150 +7 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Tissue cDNA, First Strand Diseased, cDNA Clone Full Product Name Tissue cDNA, First Strand, Human Diseased (Adult), Diabetes, Brain, BioGenomics Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Purity/Purification Molecular Biology Grade Form/Format Supplied as a liquid in 50mM Tris-CI, pH 8.3, 75mM potassium chloride, 3mM MgCI2, 10mM DTT. Bioselect Tissue cDNA cDNA is supplied as First Strand, Multiple Tissue Panels, and Matched Pairs. PCR-ready First Strand cDNA is tissue specific and are ready-to-use for gene discovery or expression analysis. Over 350 cDNAs from human adult and fetal normal tissues, human diseased and tumor tissues, rat, mouse, monkey and plant tissues are included in this extensive collection.PCR Ready First Strand cDNAs is an excellent source of tissue specific, PCR-ready cDNA, and it can be immediately used for gene discovery or expression analysis. First-Strand cDNA is synthesized from RNA isolated from a wide variety of documented human adult and fetal normal tissues, human diseased and tumor tissues, mouse, rat, monkey, plant and other tissues. Total RNA used for cDNA synthesis is isolated by modified guanidine thiocyanate techniques. 11ug total RNA was primed by an oligo dT primer and reverse transcribed by MMLV reverse transcriptase in 40ul final volume. RT Reaction stopped by heating at 65 degree C for 10 minutes.1ul cDNA is good for one PCR reaction. The 5' end of human clathrin cDNA (a 6kb gene) has been amplified by PCR from all of the cDNAs. Features Ready to use for PCROligo dT primer used to ensure the entire 3' end of cDNA is presentWith some cDNA used as templates, 12kb PCR amplicon was obtained to ensure the intactness of cDNAThe largest selection of cDNAs from different tissues on the marketDocumentation of tissues' clinical histories available (additional cost) Quality Control 1. The integrity of the RNA used for cDNA synthesis is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA when electrophoresed on a denaturing agarose gel. The quality and purity of total RNA were tested by spectrophotometer. A260/280 is between 1.8 and 2.0 (detected in 10mM Tris-Cl, pH 7.5). The ratio of 28S/18S is 1.2. The RNA used for cDNA synthesis is treated by DNase I, and is tested as DNA free RNA by PCR.3. The synthesized human, animal, and cell line cDNA was 5' selected to ensure its full length. The cDNA was used as template for PCR amplification of -actin gene and an 838 bp -actin band was visualized on 1% agarose gel. -actin control primer is included. It is enough for 10 PCR reactions.4. The synthesized plant cDNA was used as template for PCR amplification of chloroplast gene. A 458 bp chloroplast band was visualized on 1% agarose gel. Chloroplast control primer is included. It is enough for 10 PCR reactions. Preparation and Storage Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months. Other Notes Small volumes of Tissue cDNA, First Strand Diseased cdna clone vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Product Categories/Family for Tissue cDNA, First Strand Diseased cdna clone Cloning; Cloning-cDNA Application Notes for Tissue cDNA, First Strand Diseased cdna clone Immediate PCR Amplification of known genes, Verification of genetic mutation, Comparison of a specific gene between different tissues, Analysis of mRNA alternative splicing, Gene cloning and target sequencing TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP