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| Product Name
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ABCC8, siRNA
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| Also Known As
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ABCC8 siRNA (Rat)
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| Product Synonym Names
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SUR; SUR1; ATP-binding cassette sub-family C member 8; Sulfonylurea receptor 1
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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 TOP
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| 3D Structure
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ModBase 3D Structure for Q09429
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| Host
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Synthetic
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| Species Reactivity
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Rat
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| Specificity
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ABCC8 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
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| Purity/Purification
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> 97%
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| Form/Format
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Lyophilized powder
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| Quality Control
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Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
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| Directions for Use
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We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
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| Components
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We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ABCC8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
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| Preparation and Storage
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Shipped at 4 degree C. Store at -20 degree C for one year.
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| Negative Control
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siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
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| Other Notes
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Small volumes of ABCC8 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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TOP
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Related Product Information for ABCC8 sirna
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siRNA to inhibit ABCC8 expression using RNA interference
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| Applications Tested/Suitable for ABCC8 sirna
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RNA Interference (RNAi)
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TOP
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| NCBI/Uniprot data below describe general gene information for ABCC8. It may not necessarily be applicable to this product. |
| NCBI GI #
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148368981
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| NCBI GeneID
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25559
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| NCBI Accession #
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NP_037171.2
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_013039.2
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[Other Products]
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| UniProt Primary Accession #
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Q09429
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[Other Products]
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| UniProt Secondary Accession #
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O54989; P70532; Q9EQT0
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[Other Products]
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| UniProt Related Accession #
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Q09429
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[Other Products]
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| Molecular Weight
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177,097 Da
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TOP
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| NCBI Official Full Name
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ATP-binding cassette sub-family C member 8
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| NCBI Official Synonym Full Names
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ATP-binding cassette, subfamily C (CFTR/MRP), member 8
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| NCBI Official Symbol
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Abcc8
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[Similar Products]
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| NCBI Official Synonym Symbols
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Sur; Sur1
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[Similar Products]
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| NCBI Protein Information
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ATP-binding cassette sub-family C member 8
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| UniProt Protein Name
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ATP-binding cassette sub-family C member 8
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| UniProt Synonym Protein Names
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Sulfonylurea receptor 1
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| Protein Family
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ABC transporter C family
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| UniProt Gene Name
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Abcc8
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[Similar Products]
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| UniProt Synonym Gene Names
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Sur; Sur1
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[Similar Products]
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| UniProt Entry Name
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ABCC8_RAT
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| NCBI Summary for ABCC8
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binds sulfonylurea; subunit of the ATP-sensitive potassium channel K(ATP), which is composed of Sur1 and the inwardly rectifying K(+) channel subunit, Kir6.2 [RGD, Feb 2006]
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| UniProt Comments for ABCC8
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Subunit of the beta-cell ATP-sensitive potassium channel (KATP). Regulator of ATP-sensitive K+ channels and insulin release.
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TOP
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| Research Articles on ABCC8
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1. Results indicate that ghrelin inhibits matrix metalloprotease-9, sulfonylurea receptor 1 and transient receptor potential melastatin 4 expressions, thereby reducing blood spinal cord barrier permeability and hemorrhage after spinal cord injury.
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TOP
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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TOP
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| Products associated with ABCC8 sirna | Pathways associated with ABCC8 sirna |
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| Diseases associated with ABCC8 sirna | Organs/Tissues associated with ABCC8 sirna |
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TOP
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siRNA 
