Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow siRNA rightarrow ANPEP  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Uncharacterized symporter yihO (yihO) Recombinant Protein
 • EVL cDNA Clone
 • Annexin A5 (ANXA5) ELISA Kit
 • 2-oxoisovalee dehydrogenase subunit beta, mitochondrial (BCK... ELISA Kit
 • High osmolarity signaling protein MOS1 (MOS1) Recombinant Protein
 • Microsomal triglyceride transfer protein large subunit (MTTP... Recombinant Protein
 • Procollagen Type III (PC3) ELISA Kit
 • TREM-2b (TREM2) Antibody
 • MAP2K3 - MEK3 - MKK3 Antibody
 • TALDO1 Recombinant Protein
 • Anti-Actin Antibody (AAA) ELISA Kit
 • v-mos Moloney murine sarcoma viral oncogene homolog ELISA Kit
 • oligonucleotide-oligosaccha ride-binding fold containing 2A (... ELISA Kit
 • EPB41 (Ab-660-418) Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

ANPEP sirna

Scan QR to view Datasheet Catalog #    MBS8220811 ANPEP sirna
Unit / Price
15 nmol  /  $365 +1 FREE 8GB USB
30 nmol  /  $515 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ANPEP, siRNA

 Also Known As   

ANPEP siRNA (Human)

 Product Synonym Names    APN; CD13; PEPN; Aminopeptidase N; AP-N; hAPN; Alanyl aminopeptidase; Aminopeptidase M; AP-M; Microsomal aminopeptidase; Myeloid plasma membrane glycoprotein CD13; gp150; CD antigen CD13
 Product Gene Name   

ANPEP sirna

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 OMIM    151530
 3D Structure    ModBase 3D Structure for P15144
Section Bar
 Host    Synthetic
 Species Reactivity    Human
Section Bar
 Specificity    ANPEP siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
 Purity/Purification    > 97%
 Form/Format    Lyophilized powder
Section Bar
 Quality Control    Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
 Directions for Use    We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
 Components    We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ANPEP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Section Bar
 Preparation and Storage    Shipped at 4 degree C. Store at -20 degree C for one year.
 Negative Control    siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
 Other Notes    Small volumes of ANPEP sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Product Description specifically for ANPEP sirna

   siRNA to inhibit ANPEP expression using RNA interference
 Applications Tested/Suitable for ANPEP sirna   

RNA Interference (RNAi)

Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for ANPEP. It may not necessarily be applicable to this product.
 NCBI GI #    157266300
 NCBI GeneID    290
 NCBI Accession #    NP_001141.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_001150.2 [Other Products]
Section Bar
 UniProt Primary Accession #    P15144 [Other Products]
 UniProt Secondary Accession #    Q16728; Q6GT90; Q8IUK3; Q8IVH3; Q9UCE0 [Other Products]
 UniProt Related Accession #    P15144 [Other Products]
 Molecular Weight    109,540 Da
Table BarTOPTable Bar
 NCBI Official Full Name    aminopeptidase N
 NCBI Official Synonym Full Names    alanyl (membrane) aminopeptidase
 NCBI Official Symbol    ANPEP [Similar Products]
 NCBI Official Synonym Symbols   
APN; CD13; LAP1; P150; PEPN; GP150
[Similar Products]
 NCBI Protein Information    aminopeptidase N
 UniProt Protein Name    Aminopeptidase N
 UniProt Synonym Protein Names   
Alanyl aminopeptidase; Aminopeptidase M; AP-M; Microsomal aminopeptidase; Myeloid plasma membrane glycoprotein CD13; gp150; CD_antigen: CD13
 Protein Family    Aminopeptidase
 UniProt Gene Name    ANPEP [Similar Products]
 UniProt Synonym Gene Names    APN; CD13; PEPN; AP-N; hAPN; AP-M [Similar Products]
 UniProt Entry Name    AMPN_HUMAN
Section Bar
 NCBI Summary for ANPEP    Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in this gene appear to be a cause of various types of leukemia or lymphoma. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for ANPEP    CD13: Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection. Belongs to the peptidase M1 family.

Protein type: Protease; EC 3.4.11.2; Receptor, misc.; Other Amino Acids Metabolism - glutathione; Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q25-q26

Cellular Component: extracellular space; lysosomal membrane; integral to plasma membrane; ER-Golgi intermediate compartment; cytosol; external side of plasma membrane

Molecular Function: viral receptor activity; metallopeptidase activity; zinc ion binding; receptor activity; aminopeptidase activity

Biological Process: entry of virus into host cell; cellular protein metabolic process; angiogenesis; proteolysis; cell differentiation; angiotensin maturation
Table BarTOPTable Bar
 Research Articles on ANPEP    1. This study permitted the identification of the novel human LAP1C isoform and partially unraveled the molecular basis of LAP1 regulation.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with ANPEP sirnaPathways associated with ANPEP sirna
 Reference Product  PubMed Publications
 ENPEP sirna  >1 publications with ANPEP and ENPEP
 Products by Pathway  Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 Cardiac Progenitor Differentiation Pathway antibodies  Cardiac Progenitor Differentiation Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Of Angiotensinogen To Angiotensins Pathway antibodies  Metabolism Of Angiotensinogen To Angiotensins Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
Diseases associated with ANPEP sirnaOrgans/Tissues associated with ANPEP sirna
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >4 publications with ANPEP and Carcinoma
 Adenocarcinoma Antibodies  >3 publications with ANPEP and Adenocarcinoma
 Vascular Diseases Antibodies  >2 publications with ANPEP and Vascular Diseases
 Diabetes Mellitus Antibodies  >2 publications with ANPEP and Diabetes Mellitus
 Cardiovascular Diseases Antibodies  >2 publications with ANPEP and Cardiovascular Diseases
 Immune System Diseases Antibodies  >2 publications with ANPEP and Immune System Diseases
 Nervous System Diseases Antibodies  >2 publications with ANPEP and Nervous System Diseases
 Liver Neoplasms, Experimental Antibodies  >1 publications with ANPEP and Liver Neoplasms, Experimental
 Liver Diseases Antibodies  >1 publications with ANPEP and Liver Diseases
 Breast Neoplasms Antibodies  >1 publications with ANPEP and Breast Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >6 publications with ANPEP and Blood
 Embryonic Tissue Antibodies  >4 publications with ANPEP and Embryonic Tissue
 Prostate Antibodies  >3 publications with ANPEP and Prostate
 Uterus Antibodies  >3 publications with ANPEP and Uterus
 Bone Antibodies  >3 publications with ANPEP and Bone
 Kidney Antibodies  >2 publications with ANPEP and Kidney
 Connective Tissue Antibodies  >2 publications with ANPEP and Connective Tissue
 Pancreas Antibodies  >2 publications with ANPEP and Pancreas
 Bone Marrow Antibodies  >2 publications with ANPEP and Bone Marrow
 Vascular Antibodies  >2 publications with ANPEP and Vascular
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions