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| Product Name
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ANPEP, siRNA
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| Also Known As
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ANPEP siRNA (Human)
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| Product Synonym Names
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APN; CD13; PEPN; Aminopeptidase N; AP-N; hAPN; Alanyl aminopeptidase; Aminopeptidase M; AP-M; Microsomal aminopeptidase; Myeloid plasma membrane glycoprotein CD13; gp150; CD antigen CD13
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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 TOP
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| OMIM
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151530
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| 3D Structure
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ModBase 3D Structure for P15144
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| Host
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Synthetic
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| Species Reactivity
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Human
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| Specificity
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ANPEP siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
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| Purity/Purification
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> 97%
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| Form/Format
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Lyophilized powder
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| Quality Control
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Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
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| Directions for Use
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We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
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| Components
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We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ANPEP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
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| Preparation and Storage
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Shipped at 4 degree C. Store at -20 degree C for one year.
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| Negative Control
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siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
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| Other Notes
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Small volumes of ANPEP sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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TOP
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Product Description specifically for ANPEP sirna
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siRNA to inhibit ANPEP expression using RNA interference
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| Applications Tested/Suitable for ANPEP sirna
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RNA Interference (RNAi)
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TOP
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| NCBI/Uniprot data below describe general gene information for ANPEP. It may not necessarily be applicable to this product. |
| NCBI GI #
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157266300
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| NCBI GeneID
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290
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| NCBI Accession #
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NP_001141.2
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_001150.2
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[Other Products]
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| UniProt Primary Accession #
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P15144
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[Other Products]
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| UniProt Secondary Accession #
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Q16728; Q6GT90; Q8IUK3; Q8IVH3; Q9UCE0
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[Other Products]
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| UniProt Related Accession #
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P15144
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[Other Products]
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| Molecular Weight
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109,540 Da
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TOP
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| NCBI Official Full Name
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aminopeptidase N
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| NCBI Official Synonym Full Names
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alanyl (membrane) aminopeptidase
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| NCBI Official Symbol
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ANPEP
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[Similar Products]
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| NCBI Official Synonym Symbols
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APN; CD13; LAP1; P150; PEPN; GP150
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[Similar Products]
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| NCBI Protein Information
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aminopeptidase N
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| UniProt Protein Name
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Aminopeptidase N
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| UniProt Synonym Protein Names
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Alanyl aminopeptidase; Aminopeptidase M; AP-M; Microsomal aminopeptidase; Myeloid plasma membrane glycoprotein CD13; gp150; CD_antigen: CD13
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| Protein Family
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Aminopeptidase
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| UniProt Gene Name
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ANPEP
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[Similar Products]
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| UniProt Synonym Gene Names
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APN; CD13; PEPN; AP-N; hAPN; AP-M
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[Similar Products]
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| UniProt Entry Name
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AMPN_HUMAN
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| NCBI Summary for ANPEP
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Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in this gene appear to be a cause of various types of leukemia or lymphoma. [provided by RefSeq, Jul 2008]
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| UniProt Comments for ANPEP
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CD13: Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection. Belongs to the peptidase M1 family.
Protein type: Protease; EC 3.4.11.2; Receptor, misc.; Other Amino Acids Metabolism - glutathione; Membrane protein, integral
Chromosomal Location of Human Ortholog: 15q25-q26
Cellular Component: extracellular space; lysosomal membrane; integral to plasma membrane; ER-Golgi intermediate compartment; cytosol; external side of plasma membrane
Molecular Function: viral receptor activity; metallopeptidase activity; zinc ion binding; receptor activity; aminopeptidase activity
Biological Process: entry of virus into host cell; cellular protein metabolic process; angiogenesis; proteolysis; cell differentiation; angiotensin maturation
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TOP
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| Research Articles on ANPEP
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1. This study permitted the identification of the novel human LAP1C isoform and partially unraveled the molecular basis of LAP1 regulation.
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TOP
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with ANPEP sirna | Pathways associated with ANPEP sirna |
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| Diseases associated with ANPEP sirna | Organs/Tissues associated with ANPEP sirna |
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TOP
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siRNA 
