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DDX11 sirna

Scan QR to view Datasheet Catalog #    MBS8209012 DDX11 sirna
Unit / Price
15 nmol  /  $365 +1 FREE 8GB USB
30 nmol  /  $515 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

DDX11, siRNA

 Also Known As   

DDX11 siRNA (Human)

 Product Synonym Names    CHL1; CHLR1; KRG2; Probable ATP-dependent RNA helicase DDX11; CHL1-related protein 1; hCHLR1; DEAD/H box protein 11; Keratinocyte growth factor-regulated gene 2 protein; KRG-2
 Product Gene Name   

DDX11 sirna

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    601150
 3D Structure    ModBase 3D Structure for Q96FC9
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 Host    Synthetic
 Species Reactivity    Human
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 Specificity    DDX11 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
 Purity/Purification    > 97%
 Form/Format    Lyophilized powder
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 Quality Control    Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
 Directions for Use    We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
 Components    We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DDX11 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
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 Preparation and Storage    Shipped at 4 degree C. Store at -20 degree C for one year.
 Negative Control    siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
 Other Notes    Small volumes of DDX11 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for DDX11 sirna

   siRNA to inhibit DDX11 expression using RNA interference
 Applications Tested/Suitable for DDX11 sirna   

RNA Interference (RNAi)

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NCBI/Uniprot data below describe general gene information for DDX11. It may not necessarily be applicable to this product.
 NCBI GI #    380420356
 NCBI GeneID    1663
 NCBI Accession #    NP_001244073.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001257144.1 [Other Products]
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 UniProt Primary Accession #    Q96FC9 [Other Products]
 UniProt Secondary Accession #    Q13333; Q86VQ4; Q86W62; Q92498; Q92770; Q92998; Q92999 [Other Products]
 UniProt Related Accession #    Q96FC9 [Other Products]
 Molecular Weight    32,951 Da
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 NCBI Official Full Name    probable ATP-dependent RNA helicase DDX11 isoform 3
 NCBI Official Synonym Full Names    DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11
 NCBI Official Symbol    DDX11 [Similar Products]
 NCBI Official Synonym Symbols   
CHL1; KRG2; WABS; CHLR1
[Similar Products]
 NCBI Protein Information    probable ATP-dependent RNA helicase DDX11
 UniProt Protein Name    Probable ATP-dependent RNA helicase DDX11
 UniProt Synonym Protein Names   
CHL1-related protein 1; hCHLR1; DEAD/H box protein 11; Keratinocyte growth factor-regulated gene 2 protein; KRG-2
 Protein Family    ATP-dependent DNA helicase
 UniProt Gene Name    DDX11 [Similar Products]
 UniProt Synonym Gene Names    CHL1; CHLR1; KRG2; hCHLR1; KRG-2 [Similar Products]
 UniProt Entry Name    DDX11_HUMAN
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 NCBI Summary for DDX11    DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an enzyme that possesses both ATPase and DNA helicase activities. This gene is a homolog of the yeast CHL1 gene, and may function to maintain chromosome transmission fidelity and genome stability. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
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 UniProt Comments for DDX11    DDX11: DNA helicase involved in cellular proliferation. Possesses DNA-dependent ATPase and helicase activities. This helicase translocates on single-stranded DNA in the 5' to 3' direction in the presence of ATP and, to a lesser extent, dATP. Its unwinding activity requires a 5'-single-stranded region for helicase loading, since flush-ended duplex structures do not support unwinding. The helicase activity is capable of displacing duplex regions up to 100 bp, which can be extended to 500 bp by RPA or the cohesion establishment factor, the Ctf18-RFC (replication factor C) complex activities. Stimulates the flap endonuclease activity of FEN1. Required for normal sister chromatid cohesion. Required for recruitment of bovine papillomavirus type 1 regulatory protein E2 to mitotic chrmosomes and for viral genome maintenance. Required for maintaining the chromosome segregation and is essential for embryonic development and the prevention of aneuploidy. May function during either S, G2, or M phase of the cell cycle. Binds to both single- and double-stranded DNA. Defects in DDX11 are the cause of Warsaw breakage syndrome (WBRS). It is a syndrome characterized by severe microcephaly, pre- and postnatal growth retardation, facial dysmorphism and abnormal skin pigmentation. Additional features include high arched palate, coloboma of the right optic disk, deafness, ventricular septal defect, toes and fingers abnormalities. At cellular level, drug-induced chromosomal breakage, a feature of Fanconi anemia, and sister chromatid cohesion defects, a feature of Roberts syndrome, coexist. Belongs to the DEAD box helicase family. DEAH subfamily. DDX11/CHL1 sub-subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.4.13; Nucleolus; Helicase

Chromosomal Location of Human Ortholog: 12p11

Cellular Component: nucleoplasm; spindle pole; nuclear chromatin; cytoplasm; nucleolus; midbody; nucleus

Molecular Function: ATP-dependent DNA helicase activity; DNA-dependent ATPase activity; protein binding; RNA binding; metal ion binding; 4 iron, 4 sulfur cluster binding; double-stranded DNA binding; helicase activity; single-stranded DNA binding; ATP binding

Biological Process: unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; viral reproduction; unfolded protein response; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; sister chromatid cohesion; DNA duplex unwinding

Disease: Warsaw Breakage Syndrome
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 Research Articles on DDX11    1. A distinct triplex DNA unwinding activity of ChlR1 helicase.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with DDX11 sirnaPathways associated with DDX11 sirna
 Reference Product  PubMed Publications
 UBC sirna  >1 publications with DDX11 and UBC
 PCNA sirna  >1 publications with DDX11 and PCNA
 FEN1 sirna  >1 publications with DDX11 and FEN1
 CHTF18 sirna  >1 publications with DDX11 and CHTF18
 Products by Pathway  Pathway Diagram
 IRE1alpha Activates Chaperones Pathway antibodies  IRE1alpha Activates Chaperones Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Unfolded Protein Response (UPR) Pathway antibodies  Unfolded Protein Response (UPR) Pathway Diagram
 XBP1(S) Activates Chaperone Genes Pathway antibodies  XBP1(S) Activates Chaperone Genes Pathway Diagram
Diseases associated with DDX11 sirnaOrgans/Tissues associated with DDX11 sirna
 Disease Name  Pubmed Publications
 WARSAW BREAKAGE SYNDROME Antibodies  >14 publications with DDX11 and WARSAW BREAKAGE SYNDROME
 Anemia Antibodies  >8 publications with DDX11 and Anemia
 Chromosome Breakage Antibodies  >3 publications with DDX11 and Chromosome Breakage
 Neurologic Manifestations Antibodies  >2 publications with DDX11 and Neurologic Manifestations
 Cardiovascular Diseases Antibodies  >2 publications with DDX11 and Cardiovascular Diseases
 Cell Transformation, Neoplastic Antibodies  >1 publications with DDX11 and Cell Transformation, Neoplastic
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >4 publications with DDX11 and Skin
 Blood Antibodies  >1 publications with DDX11 and Blood
 Placenta Antibodies  >1 publications with DDX11 and Placenta
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