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FBF1 sirna

Scan QR to view Datasheet Catalog #    MBS8210157 FBF1 sirna
Unit / Price
15 nmol  /  $365 +1 FREE 8GB USB
30 nmol  /  $515 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

FBF1, siRNA

 Also Known As   

FBF1 siRNA (Mouse)

 Product Synonym Names    Fas-binding factor 1; FBF-1
 Product Gene Name   

FBF1 sirna

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for A2A870
 Host    Synthetic
 Species Reactivity    Mouse
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 Specificity    FBF1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
 Purity/Purification    > 97%
 Form/Format    Lyophilized powder
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 Quality Control    Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
 Directions for Use    We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
 Components    We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse FBF1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
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 Preparation and Storage    Shipped at 4 degree C. Store at -20 degree C for one year.
 Negative Control    siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
 Other Notes    Small volumes of FBF1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for FBF1 sirna

   siRNA to inhibit FBF1 expression using RNA interference
 Applications Tested/Suitable for FBF1 sirna   

RNA Interference (RNAi)

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NCBI/Uniprot data below describe general gene information for FBF1. It may not necessarily be applicable to this product.
 NCBI GI #    144922653
 NCBI GeneID    217335
 NCBI Accession #    NP_766159.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_172571.3 [Other Products]
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 UniProt Primary Accession #    A2A870 [Other Products]
 UniProt Secondary Accession #    Q8BSP0; Q99MZ9; A2A871; B2RQ77 [Other Products]
 UniProt Related Accession #    A2A870 [Other Products]
 Molecular Weight    45,315 Da
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 NCBI Official Full Name    fas-binding factor 1
 NCBI Official Synonym Full Names    Fas (TNFRSF6) binding factor 1
 NCBI Official Symbol    Fbf1 [Similar Products]
 NCBI Official Synonym Symbols   
mKIAA1863; 5930413D20; 1110033G01Rik
[Similar Products]
 NCBI Protein Information    fas-binding factor 1
 UniProt Protein Name    Fas-binding factor 1
 Protein Family    Fas-binding factor
 UniProt Gene Name    Fbf1 [Similar Products]
 UniProt Synonym Gene Names    FBF-1 [Similar Products]
 UniProt Entry Name    FBF1_MOUSE
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 UniProt Comments for FBF1    FBF1: Keratin-binding protein required for epithelial cell polarization. Involved in apical junction complex (AJC) assembly via its interaction with PARD3. 6 isoforms of the human protein are produced by alternative splicing.

Cellular Component: centriole; apical junction complex; spindle pole; centrosome; cytoskeleton; cytoplasm; keratin filament; cell junction

Molecular Function: protein binding

Biological Process: apical junction assembly; cell projection organization and biogenesis; cilium biogenesis
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 Research Articles on FBF1    1. Keratins and the keratin-binding protein Albatross are important for epithelial cell polarization.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with FBF1 sirnaDiseases associated with FBF1 sirna
 Products by Pathway  Pathway Diagram
 Anchoring Of The Basal Body To The Plasma Membrane Pathway antibodies  Anchoring Of The Basal Body To The Plasma Membrane Pathway Diagram
 Assembly Of The Primary Cilium Pathway antibodies  Assembly Of The Primary Cilium Pathway Diagram
 Organelle Biogenesis And Maintenance Pathway antibodies  Organelle Biogenesis And Maintenance Pathway Diagram
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >1 publications with FBF1 and Necrosis
Organs/Tissues associated with FBF1 sirna
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >1 publications with FBF1 and Liver
 Muscle Antibodies  >1 publications with FBF1 and Muscle
 Heart Antibodies  >1 publications with FBF1 and Heart
 Testis Antibodies  >1 publications with FBF1 and Testis
 Embryonic Tissue Antibodies  >1 publications with FBF1 and Embryonic Tissue
 Brain Antibodies  >1 publications with FBF1 and Brain
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