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NR5A1 sirna

Scan QR to view Datasheet Catalog #    MBS8240086 NR5A1 sirna
Unit / Price
15 nmol  /  $365 +1 FREE 8GB USB
30 nmol  /  $515 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

NR5A1, siRNA

 Also Known As   

NR5A1 siRNA (Rat)

 Product Synonym Names    FTZF1; Steroidogenic factor 1; SF-1; STF-1; Adrenal 4-binding protein; Fushi tarazu factor homolog 1; Nuclear receptor subfamily 5 group A member 1; Steroid hormone receptor Ad4BP
 Product Gene Name   

NR5A1 sirna

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for P50569
 Host    Synthetic
 Species Reactivity    Rat
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 Specificity    NR5A1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
 Purity/Purification    > 97%
 Form/Format    Lyophilized powder
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 Quality Control    Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
 Directions for Use    We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
 Components    We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat NR5A1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
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 Preparation and Storage    Shipped at 4 degree C. Store at -20 degree C for one year.
 Negative Control    siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
 Other Notes    Small volumes of NR5A1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for NR5A1 sirna

   siRNA to inhibit NR5A1 expression using RNA interference
 Applications Tested/Suitable for NR5A1 sirna   

RNA Interference (RNAi)

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NCBI/Uniprot data below describe general gene information for NR5A1. It may not necessarily be applicable to this product.
 NCBI GI #    300797824
 NCBI GeneID    83826
 NCBI Accession #    NP_001178028.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001191099.1 [Other Products]
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 UniProt Primary Accession #    P50569 [Other Products]
 UniProt Related Accession #    P50569 [Other Products]
 Molecular Weight    52,087 Da
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 NCBI Official Full Name    steroidogenic factor 1
 NCBI Official Synonym Full Names    nuclear receptor subfamily 5, group A, member 1
 NCBI Official Symbol    Nr5a1 [Similar Products]
 NCBI Official Synonym Symbols   
Sf-1; Ad4bp; Ftzf1; Ad4BP/SF-1
[Similar Products]
 NCBI Protein Information    steroidogenic factor 1
 UniProt Protein Name    Steroidogenic factor 1
 UniProt Synonym Protein Names   
Adrenal 4-binding protein; Fushi tarazu factor homolog 1; Nuclear receptor subfamily 5 group A member 1; Steroid hormone receptor Ad4BP
 Protein Family    Steroidogenic factor
 UniProt Gene Name    Nr5a1 [Similar Products]
 UniProt Synonym Gene Names    Ftzf1; SF-1; STF-1 [Similar Products]
 UniProt Entry Name    STF1_RAT
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 NCBI Summary for NR5A1    transcription factor; essential for adrenal and gonadal development [RGD, Feb 2006]
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 UniProt Comments for NR5A1    STF-1: a nuclear hormone receptor of the NR5 subfamily. Essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P-450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the Muellerian inhibiting substance (AMH) gene as well as the AHCH and STAR genes.

Protein type: Nuclear receptor

Cellular Component: nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; ligand-dependent nuclear receptor activity; enzyme binding; DNA binding; zinc ion binding; sequence-specific DNA binding; double-stranded DNA binding; steroid hormone receptor activity; phospholipid binding; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; tissue development; intracellular receptor-mediated signaling pathway; adrenal gland development; positive regulation of transcription, DNA-dependent; male gonad development; reproductive process; multicellular organismal aging; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; luteinization; hormone metabolic process; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; maintenance of protein localization in nucleus; cell differentiation; female gonad development
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 Research Articles on NR5A1    1. These results suggest that the low level of DAX-1 may enhance the transcriptional activity of SF-1 and expression of genes related to glucocorticoid synthesis, which are targeted by SF-1, in adrenals from SHR.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NR5A1 sirnaPathways associated with NR5A1 sirna
 Reference Product  PubMed Publications
 Nr0b1 sirna  >77 publications with NR5A1 and Nr0b1
 Cyp11a1 sirna  >59 publications with NR5A1 and Cyp11a1
 Cyp19a1 sirna  >56 publications with NR5A1 and Cyp19a1
 Cyp17a1 sirna  >33 publications with NR5A1 and Cyp17a1
 Sox9 sirna  >30 publications with NR5A1 and Sox9
 Nr6a1 sirna  >5 publications with NR5A1 and Nr6a1
 Ddx20 sirna  >2 publications with NR5A1 and Ddx20
 Nr0b2 sirna  >2 publications with NR5A1 and Nr0b2
 Products by Pathway  Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 Nuclear Receptor Transcription Pathway antibodies  Nuclear Receptor Transcription Pathway Diagram
 Nuclear Receptors Pathway antibodies  Nuclear Receptors Pathway Diagram
 Ovarian Infertility Genes Pathway antibodies  Ovarian Infertility Genes Pathway Diagram
 Wnt Signaling Pathway NetPath antibodies  Wnt Signaling Pathway NetPath Diagram
Diseases associated with NR5A1 sirnaOrgans/Tissues associated with NR5A1 sirna
 Disease Name  Pubmed Publications
 Disorders of Sex Development Antibodies  >86 publications with NR5A1 and Disorders of Sex Development
 Urogenital Abnormalities Antibodies  >78 publications with NR5A1 and Urogenital Abnormalities
 46, XY Disorders of Sex Development Antibodies  >55 publications with NR5A1 and 46, XY Disorders of Sex Development
 Adrenal Insufficiency Antibodies  >51 publications with NR5A1 and Adrenal Insufficiency
 Gonadal Dysgenesis, 46,XY Antibodies  >45 publications with NR5A1 and Gonadal Dysgenesis, 46,XY
 Ovarian Diseases Antibodies  >38 publications with NR5A1 and Ovarian Diseases
 Primary Ovarian Insufficiency Antibodies  >28 publications with NR5A1 and Primary Ovarian Insufficiency
 Genital Diseases, Male Antibodies  >26 publications with NR5A1 and Genital Diseases, Male
 Hypospadias Antibodies  >24 publications with NR5A1 and Hypospadias
 Infertility, Female Antibodies  >17 publications with NR5A1 and Infertility, Female
 Organ/Tissue Name  Pubmed Publications
 Ovary Antibodies  >121 publications with NR5A1 and Ovary
 Brain Antibodies  >62 publications with NR5A1 and Brain
 Pituitary Gland Antibodies  >43 publications with NR5A1 and Pituitary Gland
 Spleen Antibodies  >14 publications with NR5A1 and Spleen
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