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PDE3B sirna

Scan QR to view Datasheet Catalog #    MBS8200369 PDE3B sirna
Unit / Price
15 nmol  /  $365 +1 FREE 8GB USB
30 nmol  /  $515 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PDE3B, siRNA

 Also Known As   

PDE3B siRNA (Mouse)

 Product Synonym Names    cGMP-inhibited 3'.5'-cyclic phosphodiesterase B; CGIPDE1; Cyclic GMP-inhibited phosphodiesterase B; CGI-PDE B
 Product Gene Name   

PDE3B sirna

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for Q61409
 Host    Synthetic
 Species Reactivity    Mouse
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 Specificity    PDE3B siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
 Purity/Purification    > 97%
 Form/Format    Lyophilized powder
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 Quality Control    Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
 Directions for Use    We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
 Components    We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PDE3B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
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 Preparation and Storage    Shipped at 4 degree C. Store at -20 degree C for one year.
 Negative Control    siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
 Other Notes    Small volumes of PDE3B sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for PDE3B sirna

   siRNA to inhibit PDE3B expression using RNA interference
 Applications Tested/Suitable for PDE3B sirna   

RNA Interference (RNAi)

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NCBI/Uniprot data below describe general gene information for PDE3B. It may not necessarily be applicable to this product.
 NCBI GI #    112983648
 NCBI GeneID    18576
 NCBI Accession #    NP_035185.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_011055.2 [Other Products]
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 UniProt Primary Accession #    Q61409 [Other Products]
 UniProt Secondary Accession #    Q8CIX5; Q9Z1J9 [Other Products]
 UniProt Related Accession #    Q61409 [Other Products]
 Molecular Weight    122,154 Da
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 NCBI Official Full Name    cGMP-inhibited 3',5'-cyclic phosphodiesterase B
 NCBI Official Synonym Full Names    phosphodiesterase 3B, cGMP-inhibited
 NCBI Official Symbol    Pde3b [Similar Products]
 NCBI Official Synonym Symbols   
AI847709; 9830102A01Rik
[Similar Products]
 NCBI Protein Information    cGMP-inhibited 3',5'-cyclic phosphodiesterase B
 UniProt Protein Name    cGMP-inhibited 3',5'-cyclic phosphodiesterase B
 UniProt Synonym Protein Names   
CGIPDE1; Cyclic GMP-inhibited phosphodiesterase B; CGI-PDE B
 Protein Family    cGMP-inhibited 3',5'-cyclic phosphodiesterase
 UniProt Gene Name    Pde3b [Similar Products]
 UniProt Synonym Gene Names    CGI-PDE B [Similar Products]
 UniProt Entry Name    PDE3B_MOUSE
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 UniProt Comments for PDE3B    PDE3B: a cGMP-inhibited phosphodiesterase 1 involved in fat metabolism. Inhibition of PDE3B can augment PDE4 inhibitor-induced apoptosis in some patients with chronic lymphocytic leukemia.

Protein type: Membrane protein, multi-pass; Nucleotide Metabolism - purine; Membrane protein, integral; Phosphodiesterase; EC 3.1.4.17

Cellular Component: Golgi apparatus; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: protein kinase B binding; 3',5'-cyclic-AMP phosphodiesterase activity; cGMP-inhibited cyclic-nucleotide phosphodiesterase activity; phosphoric diester hydrolase activity; hydrolase activity; metal ion binding; phosphoprotein binding; drug binding; cAMP binding; 3',5'-cyclic-nucleotide phosphodiesterase activity

Biological Process: negative regulation of angiogenesis; negative regulation of lipid catabolic process; cAMP catabolic process; negative regulation of cell adhesion; angiogenesis; glucose homeostasis; signal transduction; regulation of insulin secretion; endocrine pancreas development; negative regulation of insulin secretion
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 Research Articles on PDE3B    1. these findings indicate that PDE3B deletion confers cardioprotective effects because of cAMP/PKA-induced preconditioning, which is associated with the accumulation of proteins with cardioprotective function in ICEFs.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PDE3B sirnaPathways associated with PDE3B sirna
 Reference Product  PubMed Publications
 Akt1 sirna  >3 publications with PDE3B and Akt1
 Products by Pathway  Pathway Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 Glucagon Signaling Pathway antibodies  Glucagon Signaling Pathway Diagram
 Glucagon Signaling Pathway antibodies  Glucagon Signaling Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 IGF1R Signaling Cascade Pathway antibodies  IGF1R Signaling Cascade Pathway Diagram
 IRS-mediated Signalling Pathway antibodies  IRS-mediated Signalling Pathway Diagram
 IRS-related Events Triggered By IGF1R Pathway antibodies  IRS-related Events Triggered By IGF1R Pathway Diagram
 Insulin Receptor Signalling Cascade Pathway antibodies  Insulin Receptor Signalling Cascade Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
Diseases associated with PDE3B sirnaOrgans/Tissues associated with PDE3B sirna
 Disease Name  Pubmed Publications
 Diabetes Mellitus Antibodies  >17 publications with PDE3B and Diabetes Mellitus
 Cardiovascular Diseases Antibodies  >13 publications with PDE3B and Cardiovascular Diseases
 Necrosis Antibodies  >10 publications with PDE3B and Necrosis
 Disease Models, Animal Antibodies  >8 publications with PDE3B and Disease Models, Animal
 Immune System Diseases Antibodies  >8 publications with PDE3B and Immune System Diseases
 Inflammation Antibodies  >6 publications with PDE3B and Inflammation
 Fatty Liver Antibodies  >5 publications with PDE3B and Fatty Liver
 Lung Diseases Antibodies  >5 publications with PDE3B and Lung Diseases
 Hypertension Antibodies  >3 publications with PDE3B and Hypertension
 Liver Diseases Antibodies  >2 publications with PDE3B and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Adipose Tissue Antibodies  >31 publications with PDE3B and Adipose Tissue
 Pancreas Antibodies  >18 publications with PDE3B and Pancreas
 Liver Antibodies  >18 publications with PDE3B and Liver
 Brain Antibodies  >17 publications with PDE3B and Brain
 Kidney Antibodies  >7 publications with PDE3B and Kidney
 Embryonic Tissue Antibodies  >4 publications with PDE3B and Embryonic Tissue
 Testis Antibodies  >2 publications with PDE3B and Testis
 Skin Antibodies  >1 publications with PDE3B and Skin
 Intestine Antibodies  >1 publications with PDE3B and Intestine
 Spleen Antibodies  >1 publications with PDE3B and Spleen
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