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RBM25 sirna

Scan QR to view Datasheet Catalog #    MBS8234379 RBM25 sirna
Unit / Price
15 nmol  /  $365 +1 FREE 8GB USB
30 nmol  /  $515 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

RBM25, siRNA

 Also Known As   

RBM25 siRNA (Human)

 Product Synonym Names    RNPC7; RNA-binding protein 25; Arg/Glu/Asp-rich protein of 120 kDa; RED120; Protein S164; RNA-binding motif protein 25; RNA-binding region-containing protein 7
 Product Gene Name   

RBM25 sirna

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    612427
 3D Structure    ModBase 3D Structure for P49756
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 Host    Synthetic
 Species Reactivity    Human
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 Specificity    RBM25 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
 Purity/Purification    > 97%
 Form/Format    Lyophilized powder
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 Quality Control    Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
 Directions for Use    We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
 Components    We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RBM25 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
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 Preparation and Storage    Shipped at 4 degree C. Store at -20 degree C for one year.
 Negative Control    siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
 Other Notes    Small volumes of RBM25 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for RBM25 sirna

   siRNA to inhibit RBM25 expression using RNA interference
 Applications Tested/Suitable for RBM25 sirna   

RNA Interference (RNAi)

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NCBI/Uniprot data below describe general gene information for RBM25. It may not necessarily be applicable to this product.
 NCBI GI #    55741709
 NCBI GeneID    58517
 NCBI Accession #    NP_067062.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_021239.2 [Other Products]
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 UniProt Primary Accession #    P49756 [Other Products]
 UniProt Secondary Accession #    Q2TA72; Q5XJ17; Q6P665; Q9H6A1; Q9UEQ5; Q9UIE9; A0PJL9; B2RNA8; B3KT03 [Other Products]
 UniProt Related Accession #    P49756 [Other Products]
 Molecular Weight    17,953 Da
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 NCBI Official Full Name    RNA-binding protein 25
 NCBI Official Synonym Full Names    RNA binding motif protein 25
 NCBI Official Symbol    RBM25 [Similar Products]
 NCBI Official Synonym Symbols   
S164; NET52; RNPC7; Snu71; RED120; fSAP94
[Similar Products]
 NCBI Protein Information    RNA-binding protein 25
 UniProt Protein Name    RNA-binding protein 25
 UniProt Synonym Protein Names   
Arg/Glu/Asp-rich protein of 120 kDa; RED120; Protein S164; RNA-binding motif protein 25; RNA-binding region-containing protein 7
 Protein Family    RNA-binding protein
 UniProt Gene Name    RBM25 [Similar Products]
 UniProt Synonym Gene Names    RNPC7; RED120 [Similar Products]
 UniProt Entry Name    RBM25_HUMAN
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 UniProt Comments for RBM25    RBM25: RNA-binding protein that acts as a regulator of alternative pre-mRNA splicing. Involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression. Modulates the ratio of proapoptotic BCL2L1 isoform S to antiapoptotic BCL2L1 isoform L mRNA expression. When overexpressed, stimulates proapoptotic BCL2L1 isoform S 5'-splice site (5'-ss) selection, whereas its depletion caused the accumulation of antiapoptotic BCL2L1 isoform L. Promotes BCL2L1 isoform S 5'-ss usage through the 5'-CGGGCA-3' RNA sequence. Its association with LUC7L3 promotes U1 snRNP binding to a weak 5' ss in a 5'-CGGGCA-3'-dependent manner. Binds to the exonic splicing enhancer 5'-CGGGCA-3' RNA sequence located within exon 2 of the BCL2L1 pre-mRNA. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Spliceosome; RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: nucleoplasm; spliceosome; cytoplasm; nuclear speck

Molecular Function: mRNA binding; protein binding; nucleotide binding

Biological Process: regulation of apoptosis; RNA splicing; mRNA processing; regulation of alternative nuclear mRNA splicing, via spliceosome
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 Research Articles on RBM25    1. Structure-guided mutagenesis reveals a positively charged nucleic-acid-binding surface in the RBM25 PWI domain
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products related to RBM25 siRNA (Human)
 Catalog #  Product Name  Catalog #  Product Name
 MBS1205191  Recombinant Mouse RNA-binding protein 25 (Rbm25), partial  MBS8234379  RBM25 siRNA (Human)
 MBS9128545  RBM25 Polyclonal Antibody  MBS7603404  RBM25 Rabbit Polyclonal
 MBS959509  Recombinant Human RNA-binding protein 25 (RBM25), partial  MBS8217520  RBM25 siRNA (Mouse)
Products associated with RBM25 sirnaPathways associated with RBM25 sirna
 Reference Product  PubMed Publications
 LUC7L3 sirna  >2 publications with RBM25 and LUC7L3
 Products by Pathway  Pathway Diagram
 Spliceosome Pathway antibodies  Spliceosome Pathway Diagram
 Spliceosome Pathway antibodies  Spliceosome Pathway Diagram
Diseases associated with RBM25 sirnaOrgans/Tissues associated with RBM25 sirna
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >2 publications with RBM25 and Cardiovascular Diseases
 Heart Diseases Antibodies  >2 publications with RBM25 and Heart Diseases
 Liver Diseases Antibodies  >2 publications with RBM25 and Liver Diseases
 Fibrosis Antibodies  >1 publications with RBM25 and Fibrosis
 Necrosis Antibodies  >1 publications with RBM25 and Necrosis
 Poisoning Antibodies  >1 publications with RBM25 and Poisoning
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >4 publications with RBM25 and Liver
 Bone Antibodies  >3 publications with RBM25 and Bone
 Bone Marrow Antibodies  >3 publications with RBM25 and Bone Marrow
 Heart Antibodies  >2 publications with RBM25 and Heart
 Pancreas Antibodies  >1 publications with RBM25 and Pancreas
 Muscle Antibodies  >1 publications with RBM25 and Muscle
 Embryonic Tissue Antibodies  >1 publications with RBM25 and Embryonic Tissue
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