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ROBO1 sirna

Scan QR to view Datasheet Catalog #    MBS8217359 ROBO1 sirna
Unit / Price
15 nmol  /  $365 +1 FREE 8GB USB
30 nmol  /  $515 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ROBO1, siRNA

 Also Known As   

ROBO1 siRNA (Rat)

 Product Synonym Names    Roundabout homolog 1
 Product Gene Name   

ROBO1 sirna

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for O55005
 Host    Synthetic
 Species Reactivity    Rat
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 Specificity    ROBO1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
 Purity/Purification    > 97%
 Form/Format    Lyophilized powder
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 Quality Control    Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
 Directions for Use    We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
 Components    We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ROBO1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
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 Preparation and Storage    Shipped at 4 degree C. Store at -20 degree C for one year.
 Negative Control    siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
 Other Notes    Small volumes of ROBO1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for ROBO1 sirna

   siRNA to inhibit ROBO1 expression using RNA interference
 Applications Tested/Suitable for ROBO1 sirna   

RNA Interference (RNAi)

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NCBI/Uniprot data below describe general gene information for ROBO1. It may not necessarily be applicable to this product.
 NCBI GI #    11559953
 NCBI GeneID    58946
 NCBI Accession #    NP_071524.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_022188.1 [Other Products]
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 UniProt Primary Accession #    O55005 [Other Products]
 UniProt Related Accession #    O55005 [Other Products]
 Molecular Weight    180,748 Da
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 NCBI Official Full Name    roundabout homolog 1
 NCBI Official Synonym Full Names    roundabout guidance receptor 1
 NCBI Official Symbol    Robo1 [Similar Products]
 NCBI Protein Information    roundabout homolog 1
 UniProt Protein Name    Roundabout homolog 1
 Protein Family    Roundabout
 UniProt Gene Name    Robo1 [Similar Products]
 UniProt Entry Name    ROBO1_RAT
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 NCBI Summary for ROBO1    putative axon guidance receptor [RGD, Feb 2006]
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 UniProt Comments for ROBO1    ROBO1: Receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. May be required for lung development. Belongs to the immunoglobulin superfamily. ROBO family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion

Cellular Component: cell surface; cell soma; axon; cytoplasm; plasma membrane; integral to membrane; axolemma

Molecular Function: identical protein binding; protein binding; axon guidance receptor activity; LRR domain binding

Biological Process: caspase activation; axon guidance; central nervous system development; olfactory bulb interneuron development; regulation of dendrite morphogenesis; chemorepulsion involved in postnatal olfactory bulb interneuron migration; axon midline choice point recognition; negative regulation of mammary gland epithelial cell proliferation; negative regulation of cell proliferation; negative regulation of negative chemotaxis; cell migration during sprouting angiogenesis; spinal cord development; positive regulation of axonogenesis; homophilic cell adhesion
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 Research Articles on ROBO1    1. Robo1 might not be involved in the inhibitory environment after spinal cord injury
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ROBO1 sirnaPathways associated with ROBO1 sirna
 Reference Product  PubMed Publications
 Slit2 sirna  >127 publications with ROBO1 and Slit2
 Slit1 sirna  >62 publications with ROBO1 and Slit1
 Slit3 sirna  >42 publications with ROBO1 and Slit3
 Robo3 sirna  >25 publications with ROBO1 and Robo3
 Srgap1 sirna  >3 publications with ROBO1 and Srgap1
 Srgap3 sirna  >1 publications with ROBO1 and Srgap3
 Products by Pathway  Pathway Diagram
 Activation Of Rac Pathway antibodies  Activation Of Rac Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Inactivation Of Cdc42 And Rac Pathway antibodies  Inactivation Of Cdc42 And Rac Pathway Diagram
 Netrin-1 Signaling Pathway antibodies  Netrin-1 Signaling Pathway Diagram
 Regulation Of Commissural Axon Pathfinding By Slit And Robo Pathway antibodies  Regulation Of Commissural Axon Pathfinding By Slit And Robo Pathway Diagram
 Role Of Abl In Robo-Slit Signaling Pathway antibodies  Role Of Abl In Robo-Slit Signaling Pathway Diagram
 Signaling By Robo Receptor Pathway antibodies  Signaling By Robo Receptor Pathway Diagram
Diseases associated with ROBO1 sirnaOrgans/Tissues associated with ROBO1 sirna
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >48 publications with ROBO1 and Carcinoma
 Learning Disorders Antibodies  >27 publications with ROBO1 and Learning Disorders
 Neurobehavioral Manifestations Antibodies  >26 publications with ROBO1 and Neurobehavioral Manifestations
 Lung Diseases Antibodies  >17 publications with ROBO1 and Lung Diseases
 Adenocarcinoma Antibodies  >17 publications with ROBO1 and Adenocarcinoma
 Breast Neoplasms Antibodies  >14 publications with ROBO1 and Breast Neoplasms
 Lung Neoplasms Antibodies  >13 publications with ROBO1 and Lung Neoplasms
 Disease Models, Animal Antibodies  >12 publications with ROBO1 and Disease Models, Animal
 Neoplasms, Experimental Antibodies  >7 publications with ROBO1 and Neoplasms, Experimental
 Carcinoma, Hepatocellular Antibodies  >7 publications with ROBO1 and Carcinoma, Hepatocellular
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >96 publications with ROBO1 and Brain
 Embryonic Tissue Antibodies  >60 publications with ROBO1 and Embryonic Tissue
 Kidney Antibodies  >8 publications with ROBO1 and Kidney
 Heart Antibodies  >7 publications with ROBO1 and Heart
 Ovary Antibodies  >6 publications with ROBO1 and Ovary
 Pancreas Antibodies  >3 publications with ROBO1 and Pancreas
 Dorsal Root Ganglion Antibodies  >3 publications with ROBO1 and Dorsal Root Ganglion
 Inner Ear Antibodies  >2 publications with ROBO1 and Inner Ear
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