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| Product Name
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WARS, siRNA
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| Also Known As
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WARS siRNA (Human)
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| Product Synonym Names
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IFI53; WRS; Tryptophan--tRNA ligase cytoplasmic; Interferon-induced protein 53; IFP53; Tryptophanyl-tRNA synthetase; TrpRS; hWRS
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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 TOP
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| OMIM
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191050
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| 3D Structure
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ModBase 3D Structure for P23381
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| Host
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Synthetic
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| Species Reactivity
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Human
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| Specificity
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WARS siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
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| Purity/Purification
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> 97%
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| Form/Format
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Lyophilized powder
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| Quality Control
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Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
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| Directions for Use
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We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
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| Components
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We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human WARS gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
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| Preparation and Storage
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Shipped at 4 degree C. Store at -20 degree C for one year.
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| Negative Control
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siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
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| Other Notes
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Small volumes of WARS sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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TOP
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Related Product Information for WARS sirna
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siRNA to inhibit WARS expression using RNA interference
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| Applications Tested/Suitable for WARS sirna
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RNA Interference (RNAi)
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TOP
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| NCBI/Uniprot data below describe general gene information for WARS. It may not necessarily be applicable to this product. |
| NCBI GI #
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47419914
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| NCBI GeneID
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7453
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| NCBI Accession #
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NP_004175.2
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_004184.3
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[Other Products]
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| UniProt Primary Accession #
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P23381
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[Other Products]
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| UniProt Secondary Accession #
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P78535; Q502Y0; Q53XB6; Q9UDI5; Q9UDL3; A6NGN1; A6NID3
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[Other Products]
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| UniProt Related Accession #
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P23381
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[Other Products]
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| Molecular Weight
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48,852 Da
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TOP
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| NCBI Official Full Name
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tryptophan--tRNA ligase, cytoplasmic isoform a
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| NCBI Official Synonym Full Names
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tryptophanyl-tRNA synthetase
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| NCBI Official Symbol
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WARS
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[Similar Products]
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| NCBI Official Synonym Symbols
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IFI53; IFP53; GAMMA-2
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[Similar Products]
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| NCBI Protein Information
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tryptophan--tRNA ligase, cytoplasmic
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| UniProt Protein Name
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Tryptophan--tRNA ligase, cytoplasmic
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| UniProt Synonym Protein Names
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Interferon-induced protein 53; IFP53
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| Protein Family
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Tryptophan--tRNA ligase
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| UniProt Gene Name
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WARS
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[Similar Products]
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| UniProt Synonym Gene Names
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IFI53; WRS; IFP53; TrpRS; hWRS
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[Similar Products]
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| UniProt Entry Name
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SYWC_HUMAN
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| NCBI Summary for WARS
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Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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| UniProt Comments for WARS
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WARS: Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression. Belongs to the class-I aminoacyl-tRNA synthetase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Translation; EC 6.1.1.2; Amino Acid Metabolism - tryptophan; Ligase
Chromosomal Location of Human Ortholog: 14q32.31
Cellular Component: cytoplasm; nucleus; cytosol
Molecular Function: protein binding; tryptophan-tRNA ligase activity; ATP binding
Biological Process: negative regulation of cell proliferation; tryptophanyl-tRNA aminoacylation; tRNA aminoacylation for protein translation; translation; gene expression; angiogenesis; regulation of angiogenesis
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TOP
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| Research Articles on WARS
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1. Genes within recently identified loci associated with waist-hip ratio (WHR) exhibit fat depot-specific mRNA expression, which correlates with obesity-related traits. Adipose tissue (AT) mRNA expression of 6 genes (TBX15/WARS2, STAB1, PIGC, ZNRF3, GRB14)
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TOP
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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TOP
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| Products associated with WARS sirna | Pathways associated with WARS sirna |
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| Diseases associated with WARS sirna | Organs/Tissues associated with WARS sirna |
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TOP
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siRNA 
