Molecular biology has taken a tremendous leap forward with the advent of modern genome editing. Having readily available, sequence verified and easily searchable cDNA plasmids allows the research professional to rapidly advance their cloning and genome editing projects. MyBioSource has thousands of cDNA plasmids to select from and match to individual laboratory needs.
Following gene cloning, gene sequencing is performed to ensure the completely correct amino acid sequence in each cDNA plasmid. Please refer to the online data sheets for the cDNA sequence for each clone. A sample certificate of analysis (COA) can also be requested prior to purchase. The COA may include more extensive information about the plasmid DNA sequence, cloning vector utilized, and the NBCI GenBank or Uniprot accession numbers. Researchers can then use the bioinformatics data bases for additional information about any given gene sequence, including looking for homology to other family members, species homology, chromosome location, or gene function.
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With the sequence in hand, researchers can employ common cloning software packages for the Mac or PC to identify restriction cloning sites and develop their cloning strategies. Depending on their application, researchers might decide to clone their sequence into any of a number of expression vectors to express the cDNA sequence. One application might include protein expression in E. coli, yeast, baculovirus, or mammalian hosts. In each case, each researcher will need to identify an expression vector suitable for their host type.
The selection of an expression vector and a host is an important one.
Theoretically proteins produced in yeast, mammalian cells or baculovirus hosts have more post-translational modifications than Ecoli. Post-translational modifications may, for example, include glycosylation, phosphorylation, or acetylation. Depending on the host, as well as the properties of an individual protein post-translational modifications may be absent, partial, or complete compared to their native counterpart protein. Sometimes researchers opt to empirically determine which host is most suitable for their model studies by cloning the cDNA sequence into different expression vectors and expressing small amounts of proteins in different hosts for evaluation.
Researchers also need to determine if they want to perform transient of stable transfections with their expression vectors. Whereas transient transfections are suitable for many applications, other applications such as making overexpressing cell lines will require stable transfections.
Pricing and availability
Although there has been a recent surge in cDNA suppliers in the marketplace, MyBioSource is a world leader in contracting with manufacturers for high quality cDNA plasmid clones. Brief searches through our easy to maneuver cDNA collection webpages, reveals the extent of our human cDNA catalog. Many of our cDNA clones have a short lead and are available to ship quickly. Some of our cDNA clones are manufactured and purified to order and may have longer lead times. Please contact our highly trained sales professionals at firstname.lastname@example.org for lead time and to assist you with your ordering needs. Our cDNA plasmids may be purchased directly through MyBioSource.com, through faxed requisitions to 1.858.633.0166 or through emailing directly to our sales department.
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