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Many customers may ask: when should I select a CLIA assay?

Let's start with the basics, the underlying immunoassay principles. Enzyme immunoassay (EIA) in broad terms is a biochemical immunoassay that utilizes an enzyme labeled antibody or antigen to detect a target antibody or antigen. The target is also referred as the analyte and the antigens being detected are often proteins.
Enzyme-linked immunosorbent assay (ELISA), first reported in 1971, is the most common EIA detection method. It is also among the most widely used bioanalytical methods for measuring the concentration of an analyte in solution. In ELISA, the analyte is typically detected through an observable color change generated by a chromogenic reporter and substrate.
An increasing need to detect ever-smaller amounts of target analyte molecules in liquids or wet samples led to the emergence of the chemiluminescence immunoassay (CLIA), first reported in 1981. CLIAs increased the analytical sensitivity of immunoassays by using chemiluminescent indicators such as luminol to directly label the detection antibody or antigen.

More on CLIA Kit >>>

CLIA quickly became an alternative to conventional ELISA.

Researchers often opt to select CLIA kits for quantifying low concentrations of analytes in complex samples. In the strictest sense of the word, classical CLIAs are not ELISAs because they are not enzyme-linked but rather utilize a non-enzymatic reporter. However, the general underlying principals are the same. That is, both of these plate-based biochemistry techniques detect and measure the concentration of the target analyte molecule in solution. It almost goes without saying that both CLIA and ELISA avoid the use of radioactivity, whereas in the past detecting small amounts of target analytes in samples often required radioactive techniques.

Today's CLIAs kits may incorporate both enzymatic and chemiluminescent technology.

For example, a given CLIA kit may utilize Streptavidin-HRP as the enzyme and enhanced electrochemiluminescence (ECL) as the substrate. In this case, samples may be added to well plates coated with the capture antibody followed by incubation with a streptavidin-HRP conjugated detection antibody and then a luminol chemiluminescent substrate. The resulting chemiluminsecence signal is measured in a microplate luminometer.
Chemiluminescent detection technology found in CLIA assays may be the perfect solution for researchers who need immunoassays with high sensitivity, wide linear range, and have low or limited sample volume. The wide kinetic linear range associated with CLIA can enable detection of analytes with both low and high concentrations in a cohort of samples.
For example, samples with high concentrations outside of the range of detection may need to be overly diluted in traditional ELISA sandwich or competitive assays. In contrast, the range of the standard curve is wide in CLIA assays and the same samples may require less dilution. Hence, CLIA may very well enable the researcher to detect the target analyte molecule in different samples that have widely differing concentrations using the same dilution fold for all of the samples. This is just one advantage CLIA may have over ELISA. Using the same dilution for all samples reduces assay variability and is more convenient.

Additionally, the wider dynamic range of CLIA compared to ELISA generally means that less sample is consumed.

Since lower levels of target can be detected, one simply does not need as much sample. Sample types for both CLIA and ELISA may include urine, serum, plasma, tissue homogenate, feces, tissue culture supernatant, saliva or cell lysates. The suitable sample types will be listed on the kit manuals. MyBioSource offers over 1000 CLIA Kits. If your sample type is not listed for the particular kit you are interested in, please contact MyBioSource and inquire about the suitability of your sample type.
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Displaying 1 to 17 (of 17 products) order by Products Name. Result Pages:  1 

Kallikrein 10 (KLK10), CLIA Kit

(#MBS2025322)
• 
Kallikrein 10 (KLK10) CLIA Kit
Gene Names: KLK10; NES1; PRSSL1
Other Names: Kallikrein-Related Peptidase 10; Normal epithelial cell-specific 1; Protease serine-like 1; kallikrein-10 preproprotein
Host/Reactivities: Reactivity: Human
Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
Assay Type: Competitive Inhibition
Detection Range: 117.2-30,000pg/mL
Sensitivity: < 41.3pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
Special Promotion
• 24-Strip-Wells   $305
• 48-Strip-Wells   $575
• 96-Strip-Wells   $785
• 5x96-Strip-Wells   $3190
• 10x96-Strip-Wells   $5755

Kallikrein 3 (KLK3), CLIA Kit

(#MBS2025240)
• 
Kallikrein 3 (KLK3) CLIA Kit
Gene Names: KLK3
Other Names: APS; KLK2A1; PSA; HK3; KLKB1; Seminin; Prostate Specific Antigen; Kallikrein-Related Peptidase 3; Semenogelase; g-Seminoprotein; P-30 Antigen; Plasma kallikrein
Host/Reactivities: Reactivity: Human
Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
Assay Type: Double-antibody Sandwich
Detection Range: 54.9-40,000pg/mL
Sensitivity: < 19.1pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
Special Promotion
• 24-Strip-Wells   $305
• 48-Strip-Wells   $575
• 96-Strip-Wells   $785
• 5x96-Strip-Wells   $3190
• 10x96-Strip-Wells   $5755

Kallikrein 7 (KLK7), CLIA Kit

(#MBS2025329)
• 
Kallikrein 7 (KLK7) CLIA Kit
Gene Names: KLK7; hK7; SCCE; PRSS6
Other Names: Kallikrein-Related Peptidase 7; Serine protease 6; Stratum corneum chymotryptic enzyme; kallikrein-7 isoform 3
Host/Reactivities: Reactivity: Human
Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
Assay Type: Double-antibody Sandwich
Detection Range: 41.2-30,000pg/mL
Sensitivity: < 18.4pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
Special Promotion
• 24-Strip-Wells   $305
• 48-Strip-Wells   $575
• 96-Strip-Wells   $785
• 5x96-Strip-Wells   $3190
• 10x96-Strip-Wells   $5755

KERA (Keratocan), CLIA Kit

(#MBS2531896)
• 
Human KERA (Keratocan) CLIA Kit
Gene Names: KERA; KTN; CNA2; SLRR2B
Other Names: keratocan
Host/Reactivities: Reactivity: Human
Assay Type: Sandwich
Detection Range: 62.5-4000pg/mL
Sensitivity: Min: 37.5pg/mL; Max: 4000pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
• 48-Strip-Wells   $450
• 96-Strip-Wells   $535
• 5x96-Strip-Wells   $2255
• 10x96-Strip-Wells   $3975

Kidney Injury Molecule 1 (Kim1), CLIA Kit

(#MBS2000415)
• 
Kidney Injury Molecule 1 (Kim1) CLIA Kit
Gene Names: Kim1; havcr1
Other Names: TIM1; TIMD1; HAVCR; Hepatitis A Virus Cellular Receptor 1; T Cell Immunoglobulin And Mucin Domain-Containing Protein 1; T-cell immunoglobulin mucin receptor 1; kidney injury molecule 1
Host/Reactivities: Reactivity: Human
Samples: Tissue homogenates, Urine, Cell culture supernates and Other Biological Fluids
Assay Type: Double-antibody Sandwich
Detection Range: 6.86-5,000pg/mL
Sensitivity: < 2.55pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
Special Promotion
• 24-Strip-Wells   $305
• 48-Strip-Wells   $575
• 96-Strip-Wells   $785
• 5x96-Strip-Wells   $3190
• 10x96-Strip-Wells   $5755

Kidney Injury Molecule 1 (Kim1), CLIA Kit

(#MBS2025255)
• 
Kidney Injury Molecule 1 (Kim1) CLIA Kit
Gene Names: Kim1; havcr1
Other Names: TIM1; TIMD1; HAVCR; Hepatitis A Virus Cellular Receptor 1; T Cell Immunoglobulin And Mucin Domain-Containing Protein 1; T-cell immunoglobulin mucin receptor 1; kidney injury molecule 1
Host/Reactivities: Reactivity: Mouse
Samples: Tissue homogenates, Urine, Cell culture supernates and Other Biological Fluids
Assay Type: Double-antibody Sandwich
Detection Range: 68.6-50,000pg/mL
Sensitivity: < 26.3pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
Special Promotion
• 24-Strip-Wells   $310
• 48-Strip-Wells   $590
• 96-Strip-Wells   $805
• 5x96-Strip-Wells   $3275
• 10x96-Strip-Wells   $5905

Kidney Injury Molecule 1 (Kim1), CLIA Kit

(#MBS2000501)
• 
Kidney Injury Molecule 1 (Kim1) CLIA Kit
Gene Names: Kim1; havcr1
Other Names: TIM1; TIMD1; HAVCR; Hepatitis A Virus Cellular Receptor 1; T Cell Immunoglobulin And Mucin Domain-Containing Protein 1; T-cell immunoglobulin mucin receptor 1; kidney injury molecule 1
Host/Reactivities: Reactivity: Rat
Samples: Urine, Tissue homogenates, Cell culture supernates and Other Biological Fluids
Assay Type: Double-antibody Sandwich
Detection Range: 5.49-4,000pg/mL
Sensitivity: < 2.33pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
Special Promotion
• 24-Strip-Wells   $315
• 48-Strip-Wells   $620
• 96-Strip-Wells   $845
• 5x96-Strip-Wells   $3440
• 10x96-Strip-Wells   $6210

KIM-1 (Kidney Injury Molecule 1), CLIA Kit

(#MBS2531354)
• 
Mouse KIM-1 (Kidney Injury Molecule 1) CLIA Kit
Gene Names: KIM-1; havcr1
Other Names: HAVCR; HAVCR-1; KIM1; TIM; TIM-1; TIM1; TIMD-1; TIMD1; kidney injury molecule 1
Host/Reactivities: Reactivity: Mouse
Assay Type: Sandwich
Detection Range: 12.5-800pg/mL
Sensitivity: Min: 7.5pg/mL; Max: 800pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
• 48-Strip-Wells   $450
• 96-Strip-Wells   $535
• 5x96-Strip-Wells   $2255
• 10x96-Strip-Wells   $3975

KIM-1 (Kidney Injury Molecule 1), CLIA Kit

(#MBS2531162)
• 
Rat KIM-1 (Kidney Injury Molecule 1) CLIA Kit
Gene Names: KIM-1; havcr1
Other Names: HAVCR; HAVCR-1; KIM1; TIM; TIM-1; TIM1; TIMD-1; TIMD1; kidney injury molecule 1
Host/Reactivities: Reactivity: Rat
Assay Type: Sandwich
Detection Range: 7.813-500pg/mL
Sensitivity: Min: 4.688pg/mL; Max: 500pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
• 48-Strip-Wells   $450
• 96-Strip-Wells   $535
• 5x96-Strip-Wells   $2255
• 10x96-Strip-Wells   $3975

KIM-1 (Kidney Injury Molecule 1), CLIA Kit

(#MBS2531688)
• 
Human KIM-1 (Kidney Injury Molecule 1) CLIA Kit
Gene Names: KIM-1; havcr1
Other Names: HAVCR; HAVCR-1; KIM1; TIM; TIM-1; TIM1; TIMD-1; TIMD1; kidney injury molecule 1
Host/Reactivities: Reactivity: Human
Assay Type: Sandwich
Detection Range: 31.25-2000pg/mL
Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
• 48-Strip-Wells   $450
• 96-Strip-Wells   $535
• 5x96-Strip-Wells   $2255
• 10x96-Strip-Wells   $3975

KLK6 (Kallikrein 6), CLIA Kit

(#MBS2531794)
• 
Human KLK6 (Kallikrein 6) CLIA Kit
Gene Names: KLK6
Host/Reactivities: Reactivity: Human
Assay Type: Sandwich
Detection Range: 62.5-4000pg/mL
Sensitivity: Min: 37.5pg/mL; Max: 4000pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
• 48-Strip-Wells   $450
• 96-Strip-Wells   $535
• 5x96-Strip-Wells   $2255
• 10x96-Strip-Wells   $3975

KLK7 (Kallikrein 7), CLIA Kit

(#MBS2532466)
• 
Human KLK7 (Kallikrein 7) CLIA Kit
Gene Names: KLK7; hK7; SCCE; PRSS6
Other Names: kallikrein-7 isoform 3; kallikrein-related peptidase 7
Host/Reactivities: Reactivity: Human
Assay Type: Sandwich
Detection Range: 31.25-2000pg/mL
Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
• 48-Strip-Wells   $450
• 96-Strip-Wells   $535
• 5x96-Strip-Wells   $2255
• 10x96-Strip-Wells   $3975

KLK8 (Kallikrein 8), CLIA Kit

(#MBS2531670)
• 
Human KLK8 (Kallikrein 8) CLIA Kit
Gene Names: KLK8; NP; HNP; NRPN; PRSS19; TADG14
Other Names: kallikrein-8 isoform 4; kallikrein-related peptidase 8
Host/Reactivities: Reactivity: Human
Assay Type: Sandwich
Detection Range: 0.156-10ng/mL
Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
• 48-Strip-Wells   $450
• 96-Strip-Wells   $535
• 5x96-Strip-Wells   $2255
• 10x96-Strip-Wells   $3975

Klotho (KL), CLIA Kit

(#MBS2000648)
• 
Klotho (KL) CLIA Kit
Gene Names: KL
Other Names: klotho
Host/Reactivities: Reactivity: Human
Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
Assay Type: Double-antibody Sandwich
Detection Range: 41.2-30,000pg/mL
Sensitivity: < 16.8pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
Special Promotion
• 24-Strip-Wells   $305
• 48-Strip-Wells   $575
• 96-Strip-Wells   $785
• 5x96-Strip-Wells   $3190
• 10x96-Strip-Wells   $5755

Klotho (KL), CLIA Kit

(#MBS2000431)
• 
Klotho (KL) CLIA Kit
Gene Names: KL
Other Names: klotho
Host/Reactivities: Reactivity: Mouse
Samples: Serum, Plasma and Other Biological Fluids
Assay Type: Double-antibody Sandwich
Detection Range: 2.74-2,000pg/mL
Sensitivity: < 1.15pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
Special Promotion
• 24-Strip-Wells   $310
• 48-Strip-Wells   $590
• 96-Strip-Wells   $805
• 5x96-Strip-Wells   $3275
• 10x96-Strip-Wells   $5905

KNG1 (Kininogen 1), CLIA Kit

(#MBS2533255)
• 
Human KNG1 (Kininogen 1) CLIA Kit
Gene Names: KNG1; BK; BDK; KNG
Other Names: kininogen-1 isoform 1; kininogen 1
Host/Reactivities: Reactivity: Human
Assay Type: Sandwich
Detection Range: 6.25-400pg/mL
Sensitivity: Min: 3.75pg/mL; Max: 400pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
• 48-Strip-Wells   $450
• 96-Strip-Wells   $535
• 5x96-Strip-Wells   $2255
• 10x96-Strip-Wells   $3975

KRT10 (Keratin, Type I Cytoskeletal 10), CLIA Kit

(#MBS2533033)
• 
Human KRT10 (Keratin, Type I Cytoskeletal 10) CLIA Kit
Gene Names: KRT10; BIE; EHK; K10; KPP; BCIE; CK10
Other Names: Cytokeratin-10; Keratin-10; keratin; type I cytoskeletal 10; keratin 10; type I
Host/Reactivities: Reactivity: Human
Assay Type: Sandwich
Detection Range: 7.813-500pg/mL
Sensitivity: Min: 4.688pg/mL; Max: 500pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
• 48-Strip-Wells   $450
• 96-Strip-Wells   $535
• 5x96-Strip-Wells   $2255
• 10x96-Strip-Wells   $3975
Displaying 1 to 17 (of 17 products) Result Pages:  1 
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