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Many customers may ask: when should I select a CLIA assay?

Let's start with the basics, the underlying immunoassay principles. Enzyme immunoassay (EIA) in broad terms is a biochemical immunoassay that utilizes an enzyme labeled antibody or antigen to detect a target antibody or antigen. The target is also referred as the analyte and the antigens being detected are often proteins.
Enzyme-linked immunosorbent assay (ELISA), first reported in 1971, is the most common EIA detection method. It is also among the most widely used bioanalytical methods for measuring the concentration of an analyte in solution. In ELISA, the analyte is typically detected through an observable color change generated by a chromogenic reporter and substrate.
An increasing need to detect ever-smaller amounts of target analyte molecules in liquids or wet samples led to the emergence of the chemiluminescence immunoassay (CLIA), first reported in 1981. CLIAs increased the analytical sensitivity of immunoassays by using chemiluminescent indicators such as luminol to directly label the detection antibody or antigen.

More on CLIA Kit >>>

CLIA quickly became an alternative to conventional ELISA.

Researchers often opt to select CLIA kits for quantifying low concentrations of analytes in complex samples. In the strictest sense of the word, classical CLIAs are not ELISAs because they are not enzyme-linked but rather utilize a non-enzymatic reporter. However, the general underlying principals are the same. That is, both of these plate-based biochemistry techniques detect and measure the concentration of the target analyte molecule in solution. It almost goes without saying that both CLIA and ELISA avoid the use of radioactivity, whereas in the past detecting small amounts of target analytes in samples often required radioactive techniques.

Today's CLIAs kits may incorporate both enzymatic and chemiluminescent technology.

For example, a given CLIA kit may utilize Streptavidin-HRP as the enzyme and enhanced electrochemiluminescence (ECL) as the substrate. In this case, samples may be added to well plates coated with the capture antibody followed by incubation with a streptavidin-HRP conjugated detection antibody and then a luminol chemiluminescent substrate. The resulting chemiluminsecence signal is measured in a microplate luminometer.
Chemiluminescent detection technology found in CLIA assays may be the perfect solution for researchers who need immunoassays with high sensitivity, wide linear range, and have low or limited sample volume. The wide kinetic linear range associated with CLIA can enable detection of analytes with both low and high concentrations in a cohort of samples.
For example, samples with high concentrations outside of the range of detection may need to be overly diluted in traditional ELISA sandwich or competitive assays. In contrast, the range of the standard curve is wide in CLIA assays and the same samples may require less dilution. Hence, CLIA may very well enable the researcher to detect the target analyte molecule in different samples that have widely differing concentrations using the same dilution fold for all of the samples. This is just one advantage CLIA may have over ELISA. Using the same dilution for all samples reduces assay variability and is more convenient.

Additionally, the wider dynamic range of CLIA compared to ELISA generally means that less sample is consumed.

Since lower levels of target can be detected, one simply does not need as much sample. Sample types for both CLIA and ELISA may include urine, serum, plasma, tissue homogenate, feces, tissue culture supernatant, saliva or cell lysates. The suitable sample types will be listed on the kit manuals. MyBioSource offers over 1000 CLIA Kits. If your sample type is not listed for the particular kit you are interested in, please contact MyBioSource and inquire about the suitability of your sample type.
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Displaying 1 to 22 (of 22 products) order by Products Name. Result Pages:  1 

U-T3 (Ultrasensitivity Triiodothyronine), CLIA Kit

(#MBS2532034)
• 
Rat U-T3 (Ultrasensitivity Triiodothyronine) CLIA Kit
Gene Names: U-T3
Host/Reactivities: Reactivity: Rat
Detection Range: 15.625~1000fmol/mL

(CLIA Kit)
• 48-Strip-Wells   $450
• 96-Strip-Wells   $535
• 5x96-Strip-Wells   $2255
• 10x96-Strip-Wells   $3975

Ub (Ubiquitin), CLIA Kit

(#MBS2532825)
• 
Mouse Ub (Ubiquitin) CLIA Kit
Gene Names: Ub; UBC; HMG20
Other Names: ubiquitin; ubiquitin C
Host/Reactivities: Reactivity: Mouse
Assay Type: Sandwich
Detection Range: 12.5-800pg/mL
Sensitivity: Min: 7.5pg/mL; Max: 800pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
• 48-Strip-Wells   $450
• 96-Strip-Wells   $535
• 5x96-Strip-Wells   $2255
• 10x96-Strip-Wells   $3975

Ub (Ubiquitin), CLIA Kit

(#MBS2530537)
• 
Human Ub (Ubiquitin) CLIA Kit
Gene Names: Ub; UBC; HMG20
Other Names: ubiquitin; ubiquitin C
Host/Reactivities: Reactivity: Human
Assay Type: Sandwich
Detection Range: 15.625-1000pg/mL
Sensitivity: Min: 9.375pg/mL; Max: 1000pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
• 48-Strip-Wells   $450
• 96-Strip-Wells   $535
• 5x96-Strip-Wells   $2255
• 10x96-Strip-Wells   $3975

UBA52 (Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1), CLIA Kit

(#MBS2533060)
• 
Human UBA52 (Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1) CLIA Kit
Gene Names: UBA52
Host/Reactivities: Reactivity: Human
Assay Type: Sandwich
Detection Range: 62.5-4000pg/mL
Sensitivity: Min: 37.5pg/mL; Max: 4000pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
• 48-Strip-Wells   $450
• 96-Strip-Wells   $535
• 5x96-Strip-Wells   $2255
• 10x96-Strip-Wells   $3975

UBAP2 (Ubiquitin Associated Protein 2), CLIA Kit

(#MBS2530458)
• 
Human UBAP2 (Ubiquitin Associated Protein 2) CLIA Kit
Gene Names: UBAP2; UBAP-2
Other Names: ubiquitin-associated protein 2 isoform 1; ubiquitin associated protein 2
Host/Reactivities: Reactivity: Human
Assay Type: Sandwich
Detection Range: 62.5-4000pg/mL
Sensitivity: Min: 37.5pg/mL; Max: 4000pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
• 48-Strip-Wells   $450
• 96-Strip-Wells   $535
• 5x96-Strip-Wells   $2255
• 10x96-Strip-Wells   $3975

UBD (Ubiquitin D), CLIA Kit

(#MBS2531232)
• 
Human UBD (Ubiquitin D) CLIA Kit
Gene Names: UBD; FAT10; UBD-3; GABBR1
Other Names: ubiquitin D
Host/Reactivities: Reactivity: Human
Assay Type: Sandwich
Detection Range: 0.781-50ng/mL
Sensitivity: Min: 0.469ng/mL; Max: 50ng/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
• 48-Strip-Wells   $450
• 96-Strip-Wells   $535
• 5x96-Strip-Wells   $2255
• 10x96-Strip-Wells   $3975

UBE1L (Ubiquitin Activating Enzyme E1 Like Protein), CLIA Kit

(#MBS2532710)
• 
Human UBE1L (Ubiquitin Activating Enzyme E1 Like Protein) CLIA Kit
Gene Names: UBE1L; THAPSDRAFT_262157
Other Names: ubiquitin activating enzyme e1-like protein; partial
Host/Reactivities: Reactivity: Human
Assay Type: Sandwich
Detection Range: 0.313-20mIU/mL
Sensitivity: Min: 0.188mIU/mL; Max: 20mIU/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
• 48-Strip-Wells   $450
• 96-Strip-Wells   $535
• 5x96-Strip-Wells   $2255
• 10x96-Strip-Wells   $3975

UBE2A (Ubiquitin Conjugating Enzyme E2A), CLIA Kit

(#MBS2533223)
• 
Human UBE2A (Ubiquitin Conjugating Enzyme E2A) CLIA Kit
Gene Names: UBE2A; ubc2; hhr6a; rad6a
Other Names: ubiquitin-conjugating enzyme E2A
Host/Reactivities: Reactivity: Human
Assay Type: Sandwich
Detection Range: 62.5-4000pg/mL
Sensitivity: Min: 37.5pg/mL; Max: 4000pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
• 48-Strip-Wells   $450
• 96-Strip-Wells   $535
• 5x96-Strip-Wells   $2255
• 10x96-Strip-Wells   $3975

UBE2C (Ubiquitin Conjugating Enzyme E2C), CLIA Kit

(#MBS2530531)
• 
Human UBE2C (Ubiquitin Conjugating Enzyme E2C) CLIA Kit
Gene Names: UBE2C; UBCH10; dJ447F3.2
Other Names: Ubiquitin-conjugating enzyme E2C
Host/Reactivities: Reactivity: Human
Assay Type: Sandwich
Detection Range: 31.25-2000pg/mL
Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
• 48-Strip-Wells   $450
• 96-Strip-Wells   $535
• 5x96-Strip-Wells   $2255
• 10x96-Strip-Wells   $3975

UBE3A (Ubiquitin Protein Ligase E3A), CLIA Kit

(#MBS2530651)
• 
Human UBE3A (Ubiquitin Protein Ligase E3A) CLIA Kit
Gene Names: UBE3A; As; CG6190; das; DmelCG6190; dube3a
Other Names: ubiquitin protein ligase E3A
Host/Reactivities: Reactivity: Human
Assay Type: Sandwich
Detection Range: 62.5-4000pg/mL
Sensitivity: Min: 37.5pg/mL; Max: 4000pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
• 48-Strip-Wells   $450
• 96-Strip-Wells   $535
• 5x96-Strip-Wells   $2255
• 10x96-Strip-Wells   $3975

Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1), CLIA Kit

(#MBS2001223)
• 
Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) CLIA Kit
Gene Names: UCHL1; NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5
Other Names: Neuron cytoplasmic protein 9.5; Ubiquitin thioesterase L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Host/Reactivities: Reactivity: Rat
Samples: Serum, Plasma, Tissue homogenates, Cell lysates and Other Biological Fluids
Assay Type: Double-antibody Sandwich
Detection Range: 20.6-15,000pg/mL
Sensitivity: < 7.3pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
Special Promotion
• 24-Strip-Wells   $315
• 48-Strip-Wells   $620
• 96-Strip-Wells   $845
• 5x96-Strip-Wells   $3440
• 10x96-Strip-Wells   $6210

Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1), CLIA Kit

(#MBS2024700)
• 
Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) CLIA Kit
Gene Names: UCHL1
Host/Reactivities: Reactivity: Rat
Detection Range: 78-5,000pg/mL. The standard curve concentrations used for the CLIA’s were 5,000pg/mL, 2,500pg/mL, 1,250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL.

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
Special Promotion
• 24-Strip-Wells   $315
• 48-Strip-Wells   $620
• 96-Strip-Wells   $845
• 5x96-Strip-Wells   $3440
• 10x96-Strip-Wells   $6210

Ubiquitin Conjugating Enzyme E2L3 (UBE2L3), CLIA Kit

(#MBS2001005)
• 
Ubiquitin Conjugating Enzyme E2L3 (UBE2L3) CLIA Kit
Gene Names: UBE2L3; E2-F1; L-UBC; UBCH7; UbcM4
Other Names: Ubiquitin carrier protein L3; Ubiquitin-conjugating enzyme E2-F1; Ubiquitin-protein ligase L3; ubiquitin-conjugating enzyme E2 L3 isoform 4; ubiquitin-conjugating enzyme E2L 3
Host/Reactivities: Reactivity: Human
Samples: Tissue homogenates and Other Biological Fluids
Assay Type: Double-antibody Sandwich
Detection Range: 13.7-10,000pg/mL
Sensitivity: < 5.2pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
Special Promotion
• 24-Strip-Wells   $305
• 48-Strip-Wells   $575
• 96-Strip-Wells   $785
• 5x96-Strip-Wells   $3190
• 10x96-Strip-Wells   $5755

Ubiquitin Cross Reactive Protein (UCRP), CLIA Kit

(#MBS2000454)
• 
CLIA Kit for Ubiquitin Cross Reactive Protein (UCRP)
Gene Names: UCRP
Samples: Serum, plasma and other biological fluids
Assay Type: Sandwich

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
Special Promotion
• 24-Strip-Wells   $315
• 48-Strip-Wells   $620
• 96-Strip-Wells   $845
• 5x96-Strip-Wells   $3440
• 10x96-Strip-Wells   $6210

Ubiquitin Cross Reactive Protein (UCRP), CLIA Kit

(#MBS2000488)
• 
CLIA Kit for Ubiquitin Cross Reactive Protein (UCRP)
Gene Names: UCRP
Samples: Serum, plasma and other biological fluids
Assay Type: Sandwich

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
Special Promotion
• 24-Strip-Wells   $310
• 48-Strip-Wells   $590
• 96-Strip-Wells   $805
• 5x96-Strip-Wells   $3275
• 10x96-Strip-Wells   $5905

Ubiquitin Cross Reactive Protein (UCRP), CLIA Kit

(#MBS2001038)
• 
Ubiquitin Cross Reactive Protein (UCRP) CLIA Kit
Gene Names: UCRP
Other Names: G1P2; IFI15; ISG15; Interferon Alpha-Inducible Protein clone IFI-15K; Interferon Induced Protein 15; Interferon-induced 17 kDa protein
Host/Reactivities: Reactivity: Human
Samples: Serum, Plasma and Other Biological Fluids
Assay Type: Double-antibody Sandwich
Detection Range: 27.4-20,000pg/mL
Sensitivity: < 11.5pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
Special Promotion
• 24-Strip-Wells   $305
• 48-Strip-Wells   $575
• 96-Strip-Wells   $785
• 5x96-Strip-Wells   $3190
• 10x96-Strip-Wells   $5755

UBR4 (Ubiquitin Protein Ligase E3 Component N-Recognin 4), CLIA Kit

(#MBS2532045)
• 
Human UBR4 (Ubiquitin Protein Ligase E3 Component N-Recognin 4) CLIA Kit
Gene Names: UBR4; UBR1; JBS
Other Names: ubiquitin protein ligase E3 component n-recognin 1
Host/Reactivities: Reactivity: Human
Assay Type: Sandwich
Detection Range: 31.25-2000pg/mL
Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
• 48-Strip-Wells   $450
• 96-Strip-Wells   $535
• 5x96-Strip-Wells   $2255
• 10x96-Strip-Wells   $3975

UCHL1 (Ubiquitin Carboxyl Terminal Hydrolase L1), CLIA Kit

(#MBS2531775)
• 
Human UCHL1 (Ubiquitin Carboxyl Terminal Hydrolase L1) CLIA Kit
Gene Names: UCHL1; UCH-L1
Other Names: ubiquitin carboxyl-terminal hydrolase isozyme L1; Ubiquitin thioesterase L1
Host/Reactivities: Reactivity: Human
Assay Type: Sandwich
Detection Range: 15.625-1000pg/mL
Sensitivity: Min: 9.375pg/mL; Max: 1000pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
• 48-Strip-Wells   $450
• 96-Strip-Wells   $535
• 5x96-Strip-Wells   $2255
• 10x96-Strip-Wells   $3975

UCP-1 (Uncoupling Protein 1, Mitochondrial), CLIA Kit

(#MBS2531392)
• 
Mouse UCP-1 (Uncoupling Protein 1, Mitochondrial) CLIA Kit
Gene Names: UCP-1
Other Names: SLC25A7; Thermogenin
Host/Reactivities: Reactivity: Mouse
Assay Type: Sandwich
Detection Range: 31.25-2000pg/mL
Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
• 48-Strip-Wells   $450
• 96-Strip-Wells   $535
• 5x96-Strip-Wells   $2255
• 10x96-Strip-Wells   $3975

UCP-2 (Uncoupling Protein 2, Mitochondrial), CLIA Kit

(#MBS2531515)
• 
Human UCP-2 (Uncoupling Protein 2, Mitochondrial) CLIA Kit
Gene Names: UCP-2; ucp2; ucph; bmiq4; slc25a8
Other Names: uncoupling protein 2 (mitochondrial; proton carrier)
Host/Reactivities: Reactivity: Human
Assay Type: Sandwich
Detection Range: 31.25-2000pg/mL
Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
• 48-Strip-Wells   $450
• 96-Strip-Wells   $535
• 5x96-Strip-Wells   $2255
• 10x96-Strip-Wells   $3975

UCRP (Ubiquitin Cross Reactive Protein), CLIA Kit

(#MBS2532871)
• 
Human UCRP (Ubiquitin Cross Reactive Protein) CLIA Kit
Gene Names: UCRP
Host/Reactivities: Reactivity: Human
Assay Type: Sandwich
Detection Range: 15.625-1000pg/mL
Sensitivity: Min: 9.375pg/mL; Max: 1000pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
• 48-Strip-Wells   $450
• 96-Strip-Wells   $535
• 5x96-Strip-Wells   $2255
• 10x96-Strip-Wells   $3975

USP8 (Ubiquitin Specific Peptidase 8), CLIA Kit

(#MBS2530673)
• 
Human USP8 (Ubiquitin Specific Peptidase 8) CLIA Kit
Gene Names: USP8; UBPY; SPG59; HumORF8
Other Names: Ubiquitin specific peptidase 8
Host/Reactivities: Reactivity: Human
Assay Type: Sandwich
Detection Range: 62.5-4000pg/mL
Sensitivity: Min: 37.5pg/mL; Max: 4000pg/mL

Typical Testing Data/Standard Curve (for reference only)
(CLIA Kit)
• 48-Strip-Wells   $450
• 96-Strip-Wells   $535
• 5x96-Strip-Wells   $2255
• 10x96-Strip-Wells   $3975
Displaying 1 to 22 (of 22 products) Result Pages:  1 
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