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Monoclonal Antibody
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What is Monoclonal Antibody Purification

Monoclonal antibody purification involves collecting the media from the cultured hybridomas, centrifuging and filtering the media, and then using chromatographic or physiochemical fractionation methods to isolate the antibodies. Physiochemical fractionation involves using differential precipitation, size-exclusion or solid-phase binding of proteins based on size, charge or other chemical characteristics. These techniques include Size Exclusion Chromotography (SEC), ammonium sulfate precipitation and Ion Exchange Chromotography. Antibodies can also be purified based on class-specific affinity which involves using immobilized agents, such as proteins or lectins, that have an affinity for the isotype of the antibody of interest. Antigen-affinity chromatography can also be used to purify antibodies, but this method is not commonly used for monoclonal antibodies since the target antibody is generally the only immunoglobulin in the sample.

More on Monoclonal Antibody >>>

Applications for Monoclonal Antibodies

Monoclonal AntibodyMonclonal antibodies can be used in a variety of applications and assays, including ELISA, western blotting, immunohistochemistry and flow cytometry. ELISAs are used to detect the presence of a substance in a sample and generally involve the use of a monoclonal antibody that is immobilized on a solid surface. Western blotting uses antibodies to detect specific antigens in samples that are first separated using gel electrophoresis techniques. Immunohistochemistry uses antibodies to localize antigens in fixed cells and tissue sections. Flow cytometry is used to analyze and separate cells by labeling them with antibodies that are conjugated to fluorophores.

Monoclonal Antibody Drug Uses

Monoclonal antibodies are also used as drugs or therapeutic agents to treat cancer by binding specifically to target cells or proteins and stimulating the patient's immune system to attack those cells. Anti-cancer monoclonal antibodies can be targeted against malignant cells by several mechanisms. Radioimmunotherapy uses radioactively conjugated monoclonal antibodies to target specific cellular antigens. Antibody-directed enzyme prodrug therapy involves conjugating monoclonal antibodies to drug-activating enzymes and then administering them to patients along with a non-toxic agent. This results in the conversion of the non-toxic agent to a toxic drug that is targeted to the malignant cells and minimizes the systemic toxicity. Monoclonal antibody therapies can also be administered as immunoliposomes, which are by definition antibody-conjugated liposomes. These monoclonal antibody-conjugated liposomes carry drugs directly to the cancerous cells.
There are currently multiple monoclonal antibody therapies that have been approved by the FDA including Tositumomab for non-Hodgkins lymphoma, Ipilimumab for Melanoma and Trastuzumab for breast cancer . Omalizumab, which is a monoclonal antibody to IgE, is used to treat allergy-related asthma. Monoclonal antibody therapies are also being investigated as a possible treatments for Ebola.

Monoclonal vs Polyclonal Antibody

Monoclonal antibody production involves immunizing mice with an antigen, collecting antibody-producing B-cells from the spleen and fusing them with myeloma cells. The resulting hybridoma has characteristics of both lymphocytes and heteromyloma cells and secretes antibodies that all bind to the same specific epitope. This is one of the primary advantages that monoclonal antibodies have over polyclonal antibodies.
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Displaying 1 to 27 (of 27 products) order by Products Name. Result Pages:  1 

Qa-1b, Monoclonal Antibody

(#MBS6007408)
• 
Qa-1b (H2 T23, H2 Class I Histocompatibility Antigen D37 alpha Chain, H2 Q1, Histocompatibility 2 T Region Locus 23, MHC Class I Antigen Qa1, MHC ClassIb Qa1, OTTMUSP00000015895, Qa1)
Other Names: Anti -Qa-1b (H2 T23; H2 Class I Histocompatibility Antigen D37 alpha Chain; H2 Q1; Histocompatibility 2 T Region Locus 23; MHC Class I Antigen Qa1; MHC ClassIb Qa1; OTTMUSP00000015895; Qa1)
Host/Reactivities: Host: Mouse / Reactivity: Mouse
Purification: Affinity PurifiedPurified by Protein G affinity chromatography.
Isotype/Clone: Isotype: IgG1,k / Clone 11E331
Applications: Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Neutralization

(Antibody)
• 0.1 mg   $460

Qa-2, Monoclonal Antibody

(#MBS600376)
• 
Qa-2 (PE)
Gene Names: H2-Q8; Qa8; Qa-2; Qa-8; H-2Q8; Ms10t; MMS10-T
Other Names: Anti -Qa-2 (PE); Qa-2 cell surface antigen; histocompatibility 2; Q region locus 8
Host/Reactivities: Host: Mouse / Reactivity: Mouse
Purification: PurifiedPurified
Isotype/Clone: Isotype: IgG2a,k / Clone 5K44
Applications: Flow Cytometry (FC/FACS)

(Antibody)
• 0.1 mg   $395

Qa-2, Monoclonal Antibody

(#MBS603211)
• 
Qa-2 (FITC)
Gene Names: H2-Q8; Qa8; Qa-2; Qa-8; H-2Q8; Ms10t; MMS10-T
Other Names: Anti -Qa-2 (FITC); Qa-2 cell surface antigen; histocompatibility 2; Q region locus 8
Host/Reactivities: Host: Mouse / Reactivity: Mouse
Purification: PurifiedPurified
Isotype/Clone: Isotype: IgG2a,k / Clone 5K44
Applications: Flow Cytometry (FC/FACS)

(Antibody)
• 0.5 mg   $490

Qa-2, Monoclonal Antibody

(#MBS602216)
• 
Qa-2
Gene Names: H2-Q8; Qa8; Qa-2; Qa-8; H-2Q8; Ms10t; MMS10-T
Other Names: Anti -Qa-2; Qa-2 cell surface antigen; histocompatibility 2; Q region locus 8
Host/Reactivities: Host: Mouse / Reactivity: Mouse
Purification: PurifiedPurified
Isotype/Clone: Isotype: IgG2a,k / Clone 5K44
Applications: Flow Cytometry (FC/FACS)

(Antibody)
• 0.5 mg   $470

Qa-2, Monoclonal Antibody

(#MBS673675)
• 
Mouse Anti-Mouse Qa-2-UNLB
Other Names: Mouse Anti-Mouse Qa-2 - Purified; Unlabeled Antibody; Mouse Anti-Mouse Qa-2
Host/Reactivities: Host: Mouse / Reactivity: Mouse
Isotype/Clone: Isotype: Mouse (C3H/KBR) IgG2a kappa / Clone 695H1-9-9
Applications: Flow Cytometry

(Antibody)
• 0.5 mg   $315

Qa-2, Monoclonal Antibody

(#MBS673676)
• 
Mouse Anti-Mouse Qa-2-FITC
Other Names: Mouse Anti-Mouse Qa-2 - FITC; Mouse Anti-Mouse Qa-2
Host/Reactivities: Host: Mouse / Reactivity: Mouse
Isotype/Clone: Isotype: Mouse (C3H/KBR) IgG2a kappa / Clone 695H1-9-9
Applications: Flow Cytometry

(Antibody)
• 0.5 mg   $345

Qa-2, Monoclonal Antibody

(#MBS673677)
• 
Mouse Anti-Mouse Qa-2-BIOT
Other Names: Mouse Anti-Mouse Qa-2 - Biotin; Mouse Anti-Mouse Qa-2
Host/Reactivities: Host: Mouse / Reactivity: Mouse
Isotype/Clone: Isotype: Mouse (C3H/KBR) IgG2a kappa / Clone 695H1-9-9
Applications: Flow Cytometry

(Antibody)
• 0.5 mg   $345

Qa-2, Monoclonal Antibody

(#MBS673678)
• 
Mouse Anti-Mouse Qa-2-PE
Other Names: Mouse Anti-Mouse Qa-2 - Phycoerythrin-Labeled; Mouse Anti-Mouse Qa-2
Host/Reactivities: Host: Mouse / Reactivity: Mouse
Isotype/Clone: Isotype: Mouse (C3H/KBR) IgG2a kappa / Clone 695H1-9-9
Applications: Flow Cytometry

(Antibody)
• 0.1 mg   $250

Qa-2, Monoclonal Antibody

(#MBS601024)
• 
Qa-2 (Biotin)
Gene Names: H2-Q8; Qa8; Qa-2; Qa-8; H-2Q8; Ms10t; MMS10-T
Other Names: Anti -Qa-2 (Biotin); Qa-2 cell surface antigen; histocompatibility 2; Q region locus 8
Host/Reactivities: Host: Mouse / Reactivity: Mouse
Purification: PurifiedPurified
Isotype/Clone: Isotype: IgG2a,k / Clone 5K44
Applications: Flow Cytometry (FC/FACS)

(Antibody)
• 0.5 mg   $490

Qa2, Monoclonal Antibody

(#MBS834087)
• 
Qa2 antibody (FITC)
Gene Names: Qa2; H2-Q7; Q9; Ped; Qa7; Qa-2; Qa-7; H-2Q7
Other Names: Monoclonal Qa2; Anti-Qa2; Qa 2; Qa2 antigen; histocompatibility 2; Q region locus 7
Host/Reactivities: Host: Mouse
Isotype/Clone: Isotype: IgG2a kappa / Clone 69H1-9-9
Applications: Flow Cytometry (FC/FACS)

Flow Cytometry (FC/FACS)
(Antibody)
• 0.05 mg   $210
• 0.1 mg   $275

QARS, Monoclonal Antibody

(#MBS6012851)
• 
QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS)
Gene Names: QARS; GLNRS; PRO2195
Other Names: Anti -QARS (Glutamine--tRNA Ligase; Glutaminyl-tRNA Synthetase; GlnRS); QARS protein; partial
Host/Reactivities: Host: Mouse / Reactivity: Human
Purification: Affinity PurifiedPurified by Protein A affinity chromatography.
Isotype/Clone: Isotype: IgG2b,k / Clone 5F5
Applications: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

(Antibody)
• 0.1 mg   $525

QPCT, Monoclonal Antibody

(#MBS6003746)
• 
QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase)
Gene Names: Qpct; 5730422A13Rik
Other Names: Anti -QPCT (Glutaminyl-peptide Cyclotransferase; Glutaminyl Cyclase; Glutaminyl-tRNA Cyclotransferase; Glutamyl Cyclase); Qpct protein; glutaminyl-peptide cyclotransferase (glutaminyl cyclase)
Host/Reactivities: Host: Mouse / Reactivity: Human
Purification: Affinity PurifiedPurified by Protein A affinity chromatography.
Isotype/Clone: Isotype: IgG2a,k / Clone 4E11
Applications: ELISA (EL/EIA), Western Blot (WB)

(Antibody)
• 0.1 mg   $525

QPRT, Monoclonal Antibody

(#MBS834632)
• 
QPRT antibody
Gene Names: QPRT; QPRTase; HEL-S-90n
Other Names: Monoclonal QPRT; Anti-QPRT; Quinolinate phosphoribosyltransferase; ; nicotinate-nucleotide pyrophosphorylase
Host/Reactivities: Host: Mouse / Reactivity: Human
Purification: QPRT antibody was purified by affinity chromatography.
Isotype/Clone: Isotype: IgG2a / Clone 3B3
Applications: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)

(Antibody)
• 0.1 mL   $515

QPRT, Monoclonal Antibody

(#MBS832964)
• 
QPRT antibody
Gene Names: QPRT; QPRTase; HEL-S-90n
Other Names: Monoclonal QPRT; Anti-QPRT; Quinolinate phosphoribosyltransferase; ; nicotinate-nucleotide pyrophosphorylase
Host/Reactivities: Host: Mouse / Reactivity: Human
Purification: QPRT antibody was purified by affinity chromatography.
Isotype/Clone: Isotype: IgG1 / Clone 7A8
Applications: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB)

(Antibody)
• 0.1 mL   $515

QPRT, Monoclonal Antibody

(#MBS832973)
• 
QPRT antibody
Gene Names: QPRT; QPRTase; HEL-S-90n
Other Names: Monoclonal QPRT; Anti-QPRT; Quinolinate phosphoribosyltransferase; ; nicotinate-nucleotide pyrophosphorylase
Host/Reactivities: Host: Mouse / Reactivity: Human
Purification: QPRT antibody was purified by affinity chromatography.
Isotype/Clone: Isotype: IgG1 / Clone 4B4
Applications: Flow Cytometry (FC/FACS), Western Blot (WB)

(Antibody)
• 0.1 mL   $515

QPRT, Monoclonal Antibody

(#MBS833751)
• 
QPRT antibody
Gene Names: QPRT; QPRTase; HEL-S-90n
Other Names: Monoclonal QPRT; Anti-QPRT; Quinolinate phosphoribosyltransferase; ; nicotinate-nucleotide pyrophosphorylase
Host/Reactivities: Host: Mouse / Reactivity: Human
Purification: QPRT antibody was purified by affinity chromatography.
Isotype/Clone: Isotype: IgG1 / Clone 1C10
Applications: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

(Antibody)
• 0.1 mL   $585

QPRT, Monoclonal Antibody

(#MBS200411)
• 
QPRT antibody (clone AT24C4)
Gene Names: QPRT; QPRTase; HEL-S-90n
Other Names: Nicotinate nucleotide pyrophosphorylase (carboxylating); Nicotinate nucleotide pyrophosphorylase carboxylating; Quinolinate phosphoribosyltransferase; QAPRTase; nicotinate-nucleotide pyrophosphorylase
Host/Reactivities: Host: Mouse / Reactivity: Human
Purification: By protein-A affinity chromatography
Isotype/Clone: Isotype: IgG2a, k / Clone AT24C4
Applications: ELISA (ELISA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

(Antibody)
• 0.05 mL   $240
• 0.1 mL   $315

QPRT, Monoclonal Antibody

(#MBS834280)
• 
QPRT antibody
Gene Names: QPRT; QPRTase; HEL-S-90n
Other Names: Monoclonal QPRT; Anti-QPRT; Quinolinate phosphoribosyltransferase; ; nicotinate-nucleotide pyrophosphorylase
Host/Reactivities: Host: Mouse / Reactivity: Human
Purification: QPRT antibody was purified by affinity chromatography.
Isotype/Clone: Isotype: IgG2b / Clone 4 E5
Applications: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)

(Antibody)
• 0.1 mL   $515

QPRT, Monoclonal Antibody

(#MBS832510)
• 
QPRT antibody
Gene Names: QPRT; QPRTase; HEL-S-90n
Other Names: Monoclonal QPRT; Anti-QPRT; Quinolinate phosphoribosyltransferase; ; nicotinate-nucleotide pyrophosphorylase
Host/Reactivities: Host: Mouse / Reactivity: Human
Purification: QPRT antibody was purified by affinity chromatography.
Isotype/Clone: Isotype: IgG1 / Clone 4B3
Applications: Flow Cytometry (FC/FACS), Western Blot (WB)

(Antibody)
• 0.1 mL   $515

Quinaldic Acid, Monoclonal Antibody

(#MBS358331)
• 
Monoclonal Anti-Conjugated Quinaldic Acid antibody
Host/Reactivities: Host: Mouse
Purification: IgG purified
Isotype/Clone: Isotype: IgG / Clone 2A1

Structure
(Antibody)
• 0.1 mL   $1020

Quinidine, Monoclonal Antibody

(#MBS190389)
• 
Quinidine Monoclonal Antibody
Other Names: Anti-Quinidine; Quinidine
Host/Reactivities: Host: Mouse
Isotype/Clone: Isotype: IgG2b
Applications: Immunoassay

(Antibody)
• 0.1 mg   $195
• 0.5 mg   $330

Quinidine, Monoclonal Antibody

(#MBS190390)
• 
Quinidine Monoclonal Antibody
Other Names: Anti-Quinidine; Quinidine
Host/Reactivities: Host: Mouse
Isotype/Clone: Isotype: IgG1
Applications: Immunoassay

(Antibody)
• 0.1 mg   $195
• 0.5 mg   $330

Quinidine, Monoclonal Antibody

(#MBS190391)
• 
Quinidine Monoclonal Antibody
Other Names: Anti-Quinidine; Quinidine
Host/Reactivities: Host: Mouse
Isotype/Clone: Isotype: IgG1
Applications: Immunoassay

(Antibody)
• 0.1 mg   $195
• 0.5 mg   $330

Quinidine, Monoclonal Antibody

(#MBS190392)
• 
Quinidine Monoclonal Antibody
Other Names: Anti-Quinidine; Quinidine
Host/Reactivities: Host: Mouse
Isotype/Clone: Isotype: IgG1
Applications: Immunoassay

(Antibody)
• 0.1 mg   $195
• 0.5 mg   $330

Quinidine, Monoclonal Antibody

(#MBS190393)
• 
Quinidine Monoclonal Antibody
Other Names: Anti-Quinidine; Quinidine
Host/Reactivities: Host: Mouse
Isotype/Clone: Isotype: IgG1
Applications: Immunoassay

(Antibody)
• 0.1 mg   $195
• 0.5 mg   $330

Quinidine, Monoclonal Antibody

(#MBS531136)
• 
Quinidine antibody
Other Names: Monoclonal Quinidine; Anti-Quinidine
Host/Reactivities: Host: Mouse / Reactivity: To be determined by end-user
Isotype/Clone: Isotype: IgG1 / Clone M310119
Applications: ELISA (EIA)

(Antibody)
• 0.5 mg   $550

quinolinic acid, Monoclonal Antibody

(#MBS358005)
• 
Monoclonal anti-conjugated quinolinic acid antibody
Other Names: Conjugated Quinolinic Acid
Host/Reactivities: Host: Mouse
Purification: IgG purified
Isotype/Clone: Isotype: IgG1, Kappa / Clone QUINO6H2

Specificity Data
(Antibody)
• 0.1 mL   $1020
Displaying 1 to 27 (of 27 products) Result Pages:  1 
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