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MyBioSource PCR Kit

The powerful polymerase chain reaction (PCR) molecular biology technique enables scientists to hone in on a segment of DNA, and copy it thousands, millions or even billions of times over. This can be accomplished relatively easily and inexpensively. It is incredible that a single copy or just a few copies of a DNA molecular sequence can can be amplified into thousands or more copies of the same DNA molecule.
Since it was first developed in 1983 by Kary Mullis who won the nobel prize in Chemistry in 1993 for his invention, PCR has become an invaluable molecular biology technique. Today PCR tools, reagents, and kits are used worldwide for a variety of applications. This includes identification of pathogens and infectious agents, disease diagnosis, analysis of genetic and hereditary conditions, and cloning DNA fragments for insertion into vectors, to name a few.
In conventional PCR, the amount of accumulated PCR product is measured at the end of PCR cycles after the reaction is complete. In this traditional approach, it is not possible to determine the starting concentration of the target DNA since results are only obtained after the reaction is finished. However, the PCR amplified product can be run on an agarose gel and the intensity of the band can be compared to standards of known concentrations to obtain a semi-quantitative result. Conventional PCR amplification of DNA is widely used for cloning, sequencing and genotyping.

More on PCR Kit >>>

In contrast to the traditional technology, real time PCR in relatively quantitative.

Real time PCR, also known as the quantitative polymerase chain reaction (qPCR), measures amplification of the target DNA molecule during the reaction as the amplification is occurring. Data is continuously collected during the log/exponential phase of the PCR reaction when products are doubling at every PCR cycle. During this phase, the quantity of the PCR product is directly proportional to the amount of starting template DNA. qPCR is among the most sensitive genomic analysis technique available. Applications are broad, including pathogen detection, genotyping, viral quantitation, and quantitative gene expression analysis.
Another variation of traditional PCR is the reverse transcription polymerase chain reaction (RT-PCR). RT-PCR is used to detect gene expression through generating complimentary DNA (cDNA) transcripts from RNA. In RT-PCR, reverse transcriptase is used to reverse transcribe the RNA of interest into its complement DNA. The newly synthesized cDNA is then amplified by PCR. qPCR can be incorporated into the technique and the combined technique is described as quantitative real time PCR, real time RT-PCR or qRT-PCR.

MyBioSource is a leading PCR solution Kit provider.

Real time PCR (qPCR) and real time RT-PCR (qRT-PCR) ready to use Kits are popular among scientists because the design, including primer design for PCR amplification, positive controls and other kit components have already been optimized for detection.
Detailed methodology is explained in the Kit manuals. In brief, for many of the qPCR kits detection is based on the fluorogenic 5'nuclease assay. In this assay, DNA polymerase cleaves the primer-probe at the 5' end and separates the reporter dye from the quencher dye only when the probe binds or hybridizes to the target DNA. The cleaved reporter dye generates a fluorescent signal which is monitored in real time by the PCR detection system and the increase in fluorescence signal is proportional to the amount of PCR product. For RT-PCR, the mRNA is first transcribed into cDNA, and then a thermostable DNA polymerase is used to amplify the specific, target gene fragments by PCR.

Kits are available for infectious diseases, animal pathogens, tumor detection, genetic diseases as well as for monitoring pathogens associated with organ transplantation.

Many of the kits are compliant with ISO13485 and CE marked. The kits are compatible with most brands of real time PCR systems including Roche, BioRad, and Applied Biosystems.
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Displaying 1 to 50 (of 60 products) order by Products Name. Result Pages:  1  2  [Next >>] 

Acanthamoeba species, PCR Kit

(#MBS486259)
• 
Real-time PCR detection kit for all Acanthamoeba species

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Acinetobacter baumannii, PCR Kit

(#MBS486009)
• 
Real-time PCR detection kit for Acinetobacter baumannii

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Acinetobacter Baumannii (Ab), PCR Kit

(#MBS598177)
• 
Acinetobacter Baumannii (Ab) Real Time PCR Kit

(PCR Kit)
• 25
Tests/Kit
(Instr. I,
II
Recommended)
 
 $480
• 25
Tests/Kit
(Instr.
III, IV
Recommended)
 
 $480

Acute bee paralysis virus, PCR Kit

(#MBS486112)
• 
Real-time PCR detection kit for Acute bee paralysis virus

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

AdenoVirus Type 4, PCR Kit

(#MBS598181)
• 
AdenoVirus Type 4 Real Time PCR Kit

(PCR Kit)
• 25
Tests/Kit
(Instr. I,
II
Recommended)
 
 $505
• 25
Tests/Kit
(Instr.
III, IV
Recommended)
 
 $505

Adenovirus type B, PCR Kit

(#MBS486247)
• 
Real-time PCR detection kit for Adenovirus type B

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Adenovirus type C, PCR Kit

(#MBS486340)
• 
Real-time PCR detection kit for Adenovirus type C

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Adenovirus type D, PCR Kit

(#MBS486118)
• 
Real-time PCR detection kit for Adenovirus type D

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Adenovirus type F&G, PCR Kit

(#MBS486148)
• 
Real-time PCR detection kit for Adenovirus type F&G

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Aeromonas Hydrophila, PCR Kit

(#MBS598131)
• 
Aeromonas Hydrophila Real Time PCR Kit
Samples: Stool or water samples

(PCR Kit)
• 25
Tests/Kit
(Instr. I,
II
Recommended)
 
 $560
• 25
Tests/Kit
(Instr.
III, IV
Recommended)
 
 $560

Aeromonas hydrophila, PCR Kit

(#MBS486388)
• 
Real-time PCR detection kit for Aeromonas hydrophila

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Aeromonas salmonicida, PCR Kit

(#MBS486049)
• 
Real-time PCR detection kit for Aeromonas salmonicida

(PCR Kit)
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

AFP mRNA Expression in Peripheral Blood, PCR Kit

(#MBS598266)
• 
AFP mRNA Expression in Peripheral Blood Real Time RT-PCR Kit

(PCR Kit)
• 25
Tests/Kit
(Instr. I,
II
Recommended)
 
 $795
• 25
Tests/Kit
(Instr.
III, IV
Recommended)
 
 $795

African Horse Sickness Virus, PCR Kit

(#MBS486158)
• 
Real-time PCR detection kit for African Horse Sickness Virus

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

African Trypanosomiasis, PCR Kit

(#MBS486120)
• 
Real-time PCR detection kit for African Trypanosomiasis

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Agapornis species, PCR Kit

(#MBS486389)
• 
Real-time PCR sexing kit for Agapornis species

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Aggregatibacter actinomycetemcomitans, PCR Kit

(#MBS486012)
• 
Real-time PCR detection kit for Aggregatibacter actinomycetemcomitans

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Ajellomyces capsulata , PCR Kit

(#MBS486147)
• 
Real-time PCR detection kit for Ajellomyces capsulata

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Aleutian Disease Virus, PCR Kit

(#MBS486505)
• 
Real-time PCR detection kit for Aleutian Disease Virus

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

alpha toxin producing Clostridium perfringens , PCR Kit

(#MBS486314)
• 
Real-time PCR detection kit for alpha toxin producing Clostridium perfringens

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Anaplasma centrale, PCR Kit

(#MBS486271)
• 
Real-time PCR detection kit for Anaplasma centrale

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Anaplasma marginale, PCR Kit

(#MBS486346)
• 
Real-time PCR detection kit for Anaplasma marginale

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Anaplasma phagocytophilum, PCR Kit

(#MBS486136)
• 
Real-time PCR detection kit for Anaplasma phagocytophilum

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Anas platyrhynchos (duck), PCR Kit

(#MBS486387)
• 
Real-time PCR Advanced Species specific quantification Kit for Anas platyrhynchos (duck)

(PCR Kit)
• 100
Reactions
(Advanced
Version)
 
 $995

Anas platyrhynchos(duck), PCR Kit

(#MBS486451)
• 
Real-time PCR Species specific Detection Kit for Anas platyrhynchos(duck)

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 100
Reactions
(Advanced
Version)
 
 $705

ancylostoma duodenale, PCR Kit

(#MBS486208)
• 
Real-time PCR detection kit for ancylostoma duodenale

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Anguilla anguilla (European Eel), PCR Kit

(#MBS486017)
• 
Real-time PCR Species specific Detection Kit for Anguilla anguilla (European Eel)

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 100
Reactions
(Advanced
Version)
 
 $705

Antibiotic resistance marker blaCTX-M, PCR Kit

(#MBS486476)
• 
Real-time PCR detection kit for Antibiotic resistance marker blaCTX-M

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Antibiotic resistance marker blaCTX-M2/M9, PCR Kit

(#MBS486495)
• 
Real-time PCR detection kit for Antibiotic resistance marker blaCTX-M2/M9

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Antibiotic resistance marker blaGES, PCR Kit

(#MBS486213)
• 
Real-time PCR detection kit for Antibiotic resistance marker blaGES

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Antibiotic resistance marker blaIMP, PCR Kit

(#MBS486310)
• 
Real-time PCR detection kit for Antibiotic resistance marker blaIMP

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Antibiotic resistance marker blaKPC, PCR Kit

(#MBS486141)
• 
Real-time PCR detection kit for Antibiotic resistance marker blaKPC

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Antibiotic resistance marker blaNDM-1 , PCR Kit

(#MBS486018)
• 
Real-time PCR detection kit for Antibiotic resistance marker blaNDM-1

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Antibiotic resistance marker blaSHV, PCR Kit

(#MBS486165)
• 
Real-time PCR detection kit for Antibiotic resistance marker blaSHV

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Antibiotic resistance marker blaTEM, PCR Kit

(#MBS486421)
• 
Real-time PCR detection kit for Antibiotic resistance marker blaTEM

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Antibiotic resistance marker blaVIM, PCR Kit

(#MBS486488)
• 
Real-time PCR detection kit for Antibiotic resistance marker blaVIM

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Antibiotic resistance marker MecA, PCR Kit

(#MBS486164)
• 
Real-time PCR detection kit for Antibiotic resistance marker MecA

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Antibiotic resistance marker OXA-48, PCR Kit

(#MBS486203)
• 
Real-time PCR detection kit for Antibiotic resistance marker OXA-48

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Antibiotic resistance marker VanA, PCR Kit

(#MBS486324)
• 
Real-time PCR detection kit for Antibiotic resistance marker VanA

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Antibiotic resistance marker VanB, PCR Kit

(#MBS486262)
• 
Real-time PCR detection kit for Antibiotic resistance marker VanB

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Apium graveolens var. dulce (Celery) and Apium graveolens var. rapaceum (Celeriac), PCR Kit

(#MBS486078)
• 
Real-time PCR Species specific Detection Kit for Apium graveolens var. dulce (Celery) and Apium graveolens var. rapaceum (Celeriac)

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 100
Reactions
(Advanced
Version)
 
 $705

Ascaris lumbricoides and Ascaris suum, PCR Kit

(#MBS486116)
• 
Real-time PCR detection kit for Ascaris lumbricoides and Ascaris suum

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Aspergillus, PCR Kit

(#MBS486431)
• 
Real-time PCR detection kit for Aspergillus

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Aspergillus fumigatus, PCR Kit

(#MBS486111)
• 
Real-time PCR detection kit for Aspergillus fumigatus

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Aureobasidium pullulans, PCR Kit

(#MBS486306)
• 
Real-time PCR detection kit for Aureobasidium pullulans

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Avian adenovirus EDS76 Egg Drop Syndrome , PCR Kit

(#MBS486482)
• 
Real-time PCR detection kit for Avian adenovirus EDS76 Egg Drop Syndrome

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Avian Bornavirus, PCR Kit

(#MBS486444)
• 
Real-time PCR detection kit for Avian Bornavirus

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Avian Infectious Bronchitis Virus (IBV), PCR Kit

(#MBS486517)
• 
Real-time PCR detection kit for Avian Infectious Bronchitis Virus (IBV)

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Avian Influenza A Virus Subtype H5, PCR Kit

(#MBS486288)
• 
Real-time PCR detection kit for Avian Influenza A Virus Subtype H5

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905

Avian Influenza A Virus Subtype H6, PCR Kit

(#MBS486106)
• 
Real-time PCR detection kit for Avian Influenza A Virus Subtype H6

(PCR Kit)
• 50
Reactions
(Easy
Version-Require
PCR
Machine)
 
 $520
• 150
Reactions
(Standard
Version)
 
 $705
• 150
Reactions
(Advanced
Version)
 
 $905
Displaying 1 to 50 (of 60 products) Result Pages:  1  2  [Next >>] 
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