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Secondary Antibody

Secondary Antibody Definition

The binding of antibodies to target antigens can be visualized by either colorimetric or fluorescent methods. The methods for visualization involve either direct or indirect binding. Primary antibodies can be directly conjugated to fluorophores, enzymes or other substances that will aid in the visualization, such as GFP, biotin or horseradish peroxidase. However, when primary antibodies are directly conjugated, this limits the number of applications that they will be suitable for, as well as the sensitivity. For this reason, most researchers prefer to use conjugated secondary antibodies that will bind to the primary antibody of interest.

Showing 1 to 50 of 6677 found results

anti-Mouse IgG+HRP
Gene Names:
IgG+HRP
Host/Reactivities:
Host: Goat / Reactivity: Mouse
Applications:
Western Blot (WB)
Goat anti Rabbit IgG, Fc Fragment Specific Antibody, Peroxidase Conjugated
Host/Reactivities:
Host: Goat
Purification:
Immunoaffinity chromatography
Applications:
ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry
Goat Anti-Mouse IgG Heavy and Light Chain Antibody, Peroxidase Conjugated
Host/Reactivities:
Host: Goat
Purification:
Immunoaffinity chromatography
Applications:
ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry
Goat Anti-Mouse IgG Heavy and Light Chain Antibody, Alkaline Phosphatase Conjugated
Host/Reactivities:
Host: Goat
Purification:
Immunoaffinity chromatography
Applications:
ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry
Biotin Conjugated Goat Anti-human IgM secondary antibody
Host/Reactivities:
Host: Goat
Isotype/Clone:
Isotype: IgM
Applications:
Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB), Direct ELISA (EIA)
Rabbit anti-Human IgG Fc fragment polyclonal Antibody, HRP conjugated
Gene Names:
IgG
Other Names:
fragment crystallizable region
Host/Reactivities:
Host: Rabbit / Reactivity: Human
Purification:
Caprylic Acid Ammonium Sulfate Precipitation purified
Isotype/Clone:
Isotype: IgG
Applications:
ELISA (EIA), Western Blot (WB)
Western Blot (WB)
Mouse secondary antibody to human IgG (Fc)-HRP
Other Names:
Mouse anti-human IgG (Fc)-HRP conjugated; Mouse anti-human IgG
Host/Reactivities:
Host: Mouse / Reactivity: Human
Purification:
This product was purified from antiserum by immunoaffinity chromatography (monoclonal).
Isotype/Clone:
Isotype: IgG
Applications:
Western Blot (WB), ELISA (EIA)
Donkey anti-Rabbit IgG (H&L) - Affinity Pure, DyLight350 Conjugate, min x w/bovine, chicken, goat, guinea pig, hamster, horse, human,mouse, rat or sheep IgG
Other Names:
Donkey anti-Rabbit IgG (H&L); Affinity Pure
Host/Reactivities:
Host: Donkey / Reactivity: Rabbit.Cross Reactivity: Based on IEP, no reactivity is observed to:non-immunoglobulin rabbi...
Purification:
Affinity purified antibody is > 95% based on SDS-PAGEAffinity purified using solid phase Rabbit IgG.
Applications:
Flow Cytometry (FC/FACS), Immunofluorescence (IF), ELISA (EIA), Immunomicroscopy (IM)
  • Test Data
  • SDS-PAGE
  • Immunoelectrophoresis
    Sheep anti Human IgG3 (subclass specific)
    Host/Reactivities:
    Host: Sheep / Reactivity: Human
    Applications:
    Precipitation assays
    Mouse secondary antibody to human IgG (Fc)-Unconjugated
    Other Names:
    Mouse anti-human IgG (Fc)-Unconjugated; Mouse anti-human IgG
    Host/Reactivities:
    Host: Mouse / Reactivity: Human
    Purification:
    This product was purified from antiserum by immunoaffinity chromatography (monoclonal).
    Isotype/Clone:
    Isotype: IgG
    Applications:
    Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), ELISA (EIA)
    Goat anti Human IgM (mu chain) (Alk Phos)
    Gene Names:
    IgM
    Other Names:
    Goat anti Human Immunoglobulin M (mu chain) secondary (Alk Phos)
    Host/Reactivities:
    Host: Goat / Reactivity: No reactivity is observed to non-immunoglobulin Human serum proteins or light chains on all Human im...
    Purification:
    Affinity purified using solid phase Human IgM
    Applications:
    User optimized
    HRP Rabbit Anti-Camel IgG (H+L) Antibody
    Other Names:
    HRP Rabbit Anti-Camel IgG (H+L): N/A
    Host/Reactivities:
    Host: Rabbit
    Purification:
    Affinity Purification
    Isotype/Clone:
    Isotype: Horsedaish peroxidase conjugated IgG
    Applications:
    Western Blot (WB)
    Western Blot (WB)
    Biotin Conjugated Goat Anti-rabbit IgG secondary antibody
    Host/Reactivities:
    Host: Goat
    Isotype/Clone:
    Isotype: IgG
    Applications:
    Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB), ELISA (EIA)
    Rabbit anti-Sheep IgG polyclonal Antibody, HRP conjugated
    Gene Names:
    IgG
    Host/Reactivities:
    Host: Rabbit / Reactivity: Sheep
    Purification:
    Caprylic Acid Ammonium Sulfate Precipitation Purified
    Isotype/Clone:
    Isotype: IgG
    Applications:
    ELISA (EIA)
    Goat anti Rabbit IgG
    Gene Names:
    IgG
    Other Names:
    Goat anti Rabbit Immunoglobulin G secondary
    Host/Reactivities:
    Host: Goat / Reactivity: Rabbit
    Purification:
    Goat anti Rabbit IgG was purified by affinity chromatography.
    Applications:
    User optimized
    Goat Anti-Rabbit IgM-HRP
    Other Names:
    Goat Anti-Rabbit IgM - HRP; Goat Anti-Rabbit IgM (u heavy chain specific)
    Host/Reactivities:
    Host: Goat / Reactivity: Rabbit
    Purification:
    Affinity chromatography on rabbit IgM covalently linked to agarose
    Isotype/Clone:
    Isotype: IgG
    Applications:
    ELISA; Immunoblot; Immunofluorescence; Immunohistochemistry
    Rabbit anti-Mouse IgG Fc fragment polyclonal Antibody, HRP conjugated
    Host/Reactivities:
    Host: Rabbit / Reactivity: Mouse
    Purification:
    Caprylic Acid Ammonium Sulfate Precipitation Purified
    Isotype/Clone:
    Isotype: IgG
    Applications:
    ELISA (EIA)
    Mouse Anti-Human IgG (Fc)-HRP
    Other Names:
    Mouse Anti-Human IgG (Fc) - HRP; Mouse Anti-Human IgG (Fc) (gamma chain specific)
    Isotype/Clone:
    Isotype: Mouse IgG2bK / Clone H2
    Applications:
    ELISA (EIA)
    Testing Data
    Mouse secondary antibody to human IgG (Fc)-Biotin
    Other Names:
    Mouse anti-human IgG (Fc)-Biotin conjugated; Mouse anti-human IgG
    Host/Reactivities:
    Host: Mouse / Reactivity: Human
    Purification:
    This product was purified from antiserum by immunoaffinity chromatography (monoclonal).
    Isotype/Clone:
    Isotype: IgG
    Applications:
    Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA (EIA)
    anti-Goat IgG+HRP
    Gene Names:
    IgG+HRP
    Host/Reactivities:
    Host: Rabbit / Reactivity: Goat
    Applications:
    Western Blot (WB)
    Anti-Human IgM Monoclonal Antibody (PE/Cyanine5 Conjugated) [MHM-88]
    Gene Names:
    IgM
    Other Names:
    Human IgM; Immunoglobulin heavy constant mu; IGHM; Immunoglobulin M; Ig mu chain C region
    Host/Reactivities:
    Host: Mouse / Reactivity: Human
    Isotype/Clone:
    Isotype: IgG1, kappa / Clone MHM-88
    Applications:
    Flow Cytometry (FC/FACS)
    Flow Cytometry (FC/FACS)
    HRP Goat Anti-Mouse IgG (H+L) Antibody
    Other Names:
    HRP Goat Anti-Mouse IgG (H+L): N/A
    Host/Reactivities:
    Host: Goat
    Purification:
    Affinity Purification
    Isotype/Clone:
    Isotype: Horseradish peroxidase conjugated IgG
    Applications:
    Western Blot (WB), ELISA (EIA)
    Western Blot (WB)
    HRP Goat Anti-Rabbit IgG (H+L) Antibody
    Other Names:
    HRP Goat Anti-Rabbit IgG (H+L): N/A
    Host/Reactivities:
    Host: Goat / Reactivity: Rabbit
    Purification:
    Affinity Purification
    Isotype/Clone:
    Isotype: Horseradish peroxidase conjugated IgG
    Applications:
    ELISA, Western Blot (WB), Immunohistochemistry (IHC)
    Western Blot (WB)
    Rabbit anti Human IgG (Alk Phos)
    Gene Names:
    IgG
    Other Names:
    Rabbit anti Human Immunoglobulin G secondary (Alk Phos)
    Host/Reactivities:
    Host: Rabbit / Reactivity: Human IgG
    Purification:
    Rabbit anti Human IgG (Alk Phos) was purified by affinity chromatography.
    Applications:
    ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
    Dot Blot
    Mouse Anti-Human IgG2 (Fc)-HRP
    Other Names:
    Mouse Anti-Human IgG2 (Fc) - HRP; Mouse Anti-Human IgG2 (gamma 2 chain specific)
    Host/Reactivities:
    Host: Mouse / Reactivity: Human
    Isotype/Clone:
    Isotype: Mouse (BALB/c) IgG1 kappa / Clone 31-7-4
    Applications:
    ELISA; FLISA
    ELISA
    Mouse Anti-Human IgG4 (Fc)-BIOT
    Other Names:
    Mouse Anti-Human IgG4 (Fc) - Biotin; Mouse Anti-Human IgG4 (gamma 4 chain specific)
    Host/Reactivities:
    Host: Mouse / Reactivity: Human
    Isotype/Clone:
    Isotype: Mouse (BALB/c) IgG1 kappa / Clone HP6025
    Testing Data
    Anti-Human IgM Monoclonal Antibody (PE/Cyanine7 Conjugated) [MHM-88]
    Gene Names:
    IgM
    Other Names:
    Human IgM; Immunoglobulin heavy constant mu; IGHM; Immunoglobulin M; Ig mu chain C region
    Host/Reactivities:
    Host: Mouse / Reactivity: Human
    Isotype/Clone:
    Isotype: Mouse IgG1, κ / Clone MHM-88
    Applications:
    Flow Cytometry (FC/FACS)
    • Fluorophore
    • Flow Cytometry (FC/FACS)
      AP-Goat Anti-Rabbit IgG (H+L) Antibody
      Other Names:
      AP Goat Anti-Rabbit IgG (H+L) : N/A
      Host/Reactivities:
      Host: Goat
      Purification:
      Affinity Purification
      Applications:
      Western Blot (WB)
      HRP Goat Anti-Rat IgG (H+L) Antibody
      Other Names:
      HRP Goat Anti-Rat IgG (H+L): N/A
      Host/Reactivities:
      Host: Goat
      Purification:
      Affinity Purification
      Applications:
      Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
      FITC Goat Anti-Rabbit IgG (H+L) Antibody
      Other Names:
      FITC Goat Anti-Rabbit IgG (H+L): N/A
      Host/Reactivities:
      Host: Goat
      Purification:
      Affinity Purification
      Isotype/Clone:
      Isotype: Fluorescein conjugated IgG
      Applications:
      Immunofluorescence (IF), Flow Cytometry (FC) , Immunocytochemistry (ICC)
      Immunofluorescence (IF)
      FITC Goat Anti-Rat IgG (H+L) Antibody
      Other Names:
      FITC Goat Anti-Rat IgG (H+L): N/A
      Host/Reactivities:
      Host: Goat
      Purification:
      Affinity Purification
      Applications:
      Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
      Rabbit anti-Mouse IgG Fc fragment polyclonal Antibody, FITC conjugated
      Gene Names:
      IgG
      Host/Reactivities:
      Host: Rabbit / Reactivity: Mouse
      Purification:
      Caprylic Acid Ammonium Sulfate Precipitation Purified
      Isotype/Clone:
      Isotype: IgG
      Applications:
      ELISA (EIA)
      Western Blot (WB)
      Anti-Human IgD Monoclonal Antibody (FITC Conjugated)
      Gene Names:
      IgD
      Other Names:
      Immunoglobulin heavy constant delta; IGHD; Ig delta chain C region
      Host/Reactivities:
      Host: Mouse / Reactivity: Human
      Isotype/Clone:
      Isotype: IgG2a, kappa / Clone IA6-2
      Applications:
      Flow Cytometry (FC/FACS)
      Anti-Human IgD Monoclonal Antibody (AF488 Conjugated)
      Gene Names:
      IgD
      Other Names:
      Human IgD; Immunoglobulin heavy constant delta; IGHD; Ig delta chain C region
      Host/Reactivities:
      Host: Mouse / Reactivity: Human
      Isotype/Clone:
      Isotype: IgG2a, kappa / Clone IA6-2
      Applications:
      Flow Cytometry (FC/FACS)
      Flow Cytometry (FC/FACS)
      Mouse Anti-Human IgG2 (Fc)-PE
      Other Names:
      Mouse Anti-Human IgG2 (Fc) - Phycoerythrin-Labeled; Mouse Anti-Human IgG2 (gamma 2 chain specific)
      Host/Reactivities:
      Host: Mouse / Reactivity: Human
      Isotype/Clone:
      Isotype: Mouse (BALB/c) IgG1 Kappa / Clone HP6002
      Testing Data
      HRP Donkey Anti-Mouse IgG (H+L) Antibody
      Other Names:
      HRP Donkey Anti-Mouse IgG (H+L): N/A
      Host/Reactivities:
      Host: Mouse
      Purification:
      Affinity Purification
      Applications:
      Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
      Goat anti Rabbit IgG (Alk Phos)
      Gene Names:
      IgG
      Other Names:
      Goat anti Rabbit Immunoglobulin G secondary (Alk Phos)
      Host/Reactivities:
      Host: Goat / Reactivity: Rabbit IgG
      Purification:
      Goat anti Rabbit IgG (Alk Phos) was purified by affinity chromatography.
      Applications:
      ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
      Dot Blot
      Goat anti-Llama IgG (H&L) - Affinity Pure, Biotin Conjugate
      Other Names:
      Goat anti-Llama IgG (H&L) - Affinity Pure; immunoglobulin heavy chain variable region; partial
      Host/Reactivities:
      Host: Goat / Reactivity: Llama.Cross Reactivity: Based on IEP, no reactivity is observed to non-immunoglobulin llama seru...
      Purification:
      Affinity purified antibody is > 95% based on SDS-PAGEAffinity purified using solid phase Llama IgG.
      Applications:
      ELISA (EIA), Immunohistochemistry (IHC)
      • Goat anti-Llama IgG (H&L), Biotin Conjugate Test Data
      • SDS-PAGE
      • Immunoelectrophoresis
        Rabbit anti-Mouse IgG Fc fragment polyclonal Antibody, Biotin conjugated
        Gene Names:
        IgG
        Host/Reactivities:
        Host: Rabbit / Reactivity: Mouse
        Purification:
        Caprylic Acid Ammonium Sulfate Precipitation Purified
        Isotype/Clone:
        Isotype: IgG
        Applications:
        ELISA (EIA)
        Western Blot (WB)
        HRP Donkey Anti- Rabbit IgG (H+L) Antibody
        Other Names:
        HRP Donkey Anti-Rabbit IgG (H+L): N/A
        Host/Reactivities:
        Host: Donkey
        Purification:
        Affinity Purification
        Applications:
        Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
        HRP, Mouse Anti-Rabbit IgG HCS
        Gene Names:
        IgG
        Other Names:
        Mouse Anti-Rabbit IgG
        Host/Reactivities:
        Host: Mouse / Reactivity: Rabbit
        Purification:
        Affinity purified using solid phase Rabbit IgG(H+L) light chain with finally >95% purity based on SDS-PAGE
        Isotype/Clone:
        Isotype: Mouse IgG
        Applications:
        Western Blot (WB), Immunoprecipitation (IP)
        Western Blot (WB)
        Rhodamine (TRITC) -Goat Anti-Rabbit IgG (H+L) Antibody
        Other Names:
        Rhodamine (TRITC) -Goat Anti-Rabbit IgG (H+L): N/A
        Host/Reactivities:
        Host: Goat / Reactivity: Rabbit
        Purification:
        Affinity Purification
        Isotype/Clone:
        Isotype: TRITC conjugated IgG
        Applications:
        Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
        HRP Rabbit Anti-Chicken IgY (IgG)(H+L) Antibody
        Other Names:
        HRP Rabbit Anti-Chicken IgY (IgG)(H+L): N/A
        Host/Reactivities:
        Host: Rabbit
        Purification:
        Affinity Purification
        Applications:
        Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
        Anti-Mouse IgM Monoclonal Antibody (PE/Cyanine5 Conjugated) [RMM-1]
        Gene Names:
        IgM; Ighm; muH; Igh6; Igh-6; Igh-M; AI326478
        Other Names:
        Mouse IgM; Immunoglobulin heavy constant mu; Immunoglobulin M
        Host/Reactivities:
        Host: Rat / Reactivity: Mouse
        Isotype/Clone:
        Isotype: IgG2a, kappa / Clone RMM-1
        Applications:
        Flow Cytometry (FC/FACS)
        Anti-Mouse IgM Monoclonal Antibody (PE/Cyanine5 Conjugated) [RMM-1]
        Gene Names:
        IgM; Ighm; muH; Igh6; Igh-6; Igh-M; AI326478
        Other Names:
        Mouse IgM; Immunoglobulin heavy constant mu; Immunoglobulin M
        Host/Reactivities:
        Host: Rat / Reactivity: Mouse
        Isotype/Clone:
        Isotype: IgG2a, kappa / Clone RMM-1
        Applications:
        Flow Cytometry (FC/FACS)
        FITC-Donkey Anti Rabbit IgG (H+L)
        Host/Reactivities:
        Host: Donkey
        Applications:
        Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC)
        HRP Rabbit Anti-Goat IgG (H+L) Antibody
        Other Names:
        HRP Rabbit Anti-Goat IgG (H+L): N/A
        Host/Reactivities:
        Host: Rabbit
        Purification:
        Affinity Purification
        Applications:
        Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
        Cy3-Rabbit Anti-Goat IgG (H+L) Antibody
        Other Names:
        Cy3 Rabbit Anti-Goat IgG (H+L): N/A
        Host/Reactivities:
        Host: Rabbit
        Purification:
        Affinity Purification
        Isotype/Clone:
        Isotype: Cy3
        Applications:
        Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
        Anti-Human IgM Monoclonal Antibody (AF488 Conjugated) [MHM-88]
        Gene Names:
        IgM
        Other Names:
        Human IgM; Immunoglobulin heavy constant mu; IGHM; Immunoglobulin M; Ig mu chain C region
        Host/Reactivities:
        Host: Mouse / Reactivity: Human
        Isotype/Clone:
        Isotype: IgG1, kappa / Clone MHM-88
        Applications:
        Flow Cytometry (FC/FACS)
        FITC Goat Anti-Mouse IgG (H+L) Antibody
        Other Names:
        FITC Goat Anti-Mouse IgG (H+L): N/A
        Host/Reactivities:
        Host: Mouse
        Purification:
        Affinity Purification
        Applications:
        Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
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