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Secondary Antibody
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Secondary Antibody Definition

The binding of antibodies to target antigens can be visualized by either colorimetric or fluorescent methods. The methods for visualization involve either direct or indirect binding. Primary antibodies can be directly conjugated to fluorophores, enzymes or other substances that will aid in the visualization, such as GFP, biotin or horseradish peroxidase. However, when primary antibodies are directly conjugated, this limits the number of applications that they will be suitable for, as well as the sensitivity. For this reason, most researchers prefer to use conjugated secondary antibodies that will bind to the primary antibody of interest.

More on Secondary Antibody >>>

How to choose Secondary Antibodies

Secondary antibodies are raised against the same host species that was used to generate the primary antibody. For example, if the primary antibody was raised in rabbit, then an anti-rabbit secondary antibody that was raised in a species other than rabbit should be used. Secondary antibodies that are designed for use in Western blots and ELISA are generally conjugated to enzymes, such horseradish peroxidase or alkaline phosphatase. Biotin-conjugated secondary antibodies are also commonly used. The biotin label is small and stable, so it rarely interferes with the function of the labeled antibody. Biotin binds very strongly to avidin, and the biotin-avidin interaction can be used to further amplify the signal.
Streptavidin is purified from the bacterium Streptomyces avidinii, and although this protein does not bind quite as strongly to biotin, it is used in various detection systems because it is unglycosylated and less likely to aggregate. Streptavidin also exhibits lower non-specific binding than avidin. Each avidin or streptavidin will bind four biotins per molecule. When a biotinylated secondary antibody is used for detection, the signal can be significantly amplified by incubation with an avidin-biotin complex or a labeled streptavidin-biotin. Streptavidin can also be conjugated to detection enzymes, such as horseradish peroxidase or alkaline phosphatase, as well as fluorophores, such as GFP and R-Phycoerythrin.
While secondary antibodies can be valuable tools for amplifying signals in immunohistochemistry, additional steps are often required to prevent non-specific binding, which can make it difficult to visualize the exact location of the protein of interest. The causes of non-specific binding include interactions between the primary and/or secondary antibody and serum proteins, ionic interactions between antibodies and tissues, as well as interactions with endogenous molecules that may interfere with the detection system used.
Non-specific hydrophobic interactions can be at least partially prevented by incubating tissue sections with heat-inactivated normal serum or bovine serum albumin. However, it is important to choose a normal serum that will not interact with the primary or secondary antibodies. The best choice is generally a serum from the same host animal as the secondary antibody or a serum from a species that is unrelated to either host species. Ionic interactions can be reduced by increasing the ionic strength of the fixative and diluents. Endogenous enzyme interference can be reduced through the use of blocking reagents, such as hydrogen peroxide (for horseradish peroxidase) and levamisole (for alkaline phosphatase). When avidin-biotin based detection systems are used, interference from endogenous biotin can be reduced by first incubating the sample with avidin to block the biotin binding sites and then incubating the sample with biotin to block any additional biotin binding sites on the avidin molecule.
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Displaying 1 to 50 (of 152 products) order by Products Name. Result Pages:  1  2  3  4  [Next >>] 

secretory IgA, Secondary Antibody

(#MBS534541)
• 
Goat anti Human secretory IgA antibody
Other Names: Polyclonal Goat anti Human secretory IgA; Anti-Human secretory IgA; Secretory Immunoglobulin A
Host/Reactivities: Host: Goat / Reactivity: Specific for Secretory IgA (Human). No reaction to human IgG, IgM or Non-immunoglobulin serum protei...
Applications: ELISA (EIA), Western Blot (WB)

(Secondary Antibody)
• 1 mL   $215

Serum, Secondary Antibody

(#MBS539256)
• 
Rabbit anti Pig Serum
Other Names: Polyclonal Pig Serum; Anti-Pig Serum; Whole Porcine Serum; Swine Serum
Host/Reactivities: Host: Rabbit / Reactivity: Pig
Applications: User optimized

(Secondary Antibody)
• 2 mL   $280

Serum, Polyclonal Secondary Antibody

(#MBS539287)
• 
Rabbit anti Whole Pig Serum (IgG fraction)
Other Names: Polyclonal Pig Serum (IgG fraction); Anti-Pig Serum (IgG fraction); Whole Porcine Serum; Swine Serum
Host/Reactivities: Host: Rabbit / Reactivity: Pig
Purification: Whole Pig Serum antibody (IgG fraction) was purified by Salt fractionation and DEAE ion exchange chromatography.
Isotype/Clone: Isotype: IgG
Applications: User optimized

(Secondary Antibody)
• 20 mg   $370

Serum proteins, Polyclonal Secondary Antibody

(#MBS571848)
• 
Sheep anti Rabbit Serum proteins
Host/Reactivities: Host: Sheep / Reactivity: Rabbit
Applications: Immunoprecipitation (IP)

(Secondary Antibody)
• 1 mL   $240

Sheep anti Bovine IgA, Secondary Antibody

(#MBS539290)
• 
Sheep anti Bovine IgA
Gene Names: IgA
Other Names: Bovine Immunoglobulin A
Host/Reactivities: Host: Sheep / Reactivity: Bovine
Purification: Sheep anti Bovine IgA was purified by Immunoelectrophoresis.
Applications: ELISA (EIA), Western Blot (WB)

(Secondary Antibody)
• 1 mg   $255

Sheep anti Bovine IgG (H chain) (HRP), Secondary Antibody

(#MBS539257)
• 
Sheep anti Bovine IgG (H chain) (HRP)
Gene Names: IgG
Other Names: Bovine Immuonglobulin G (H chain) (HRP); Cow IgG (H chain) (HRP)
Host/Reactivities: Host: Sheep / Reactivity: Bovine
Purification: Sheep anti Bovine IgG (H chain) (HRP) was purified by affinity chromatography.
Isotype/Clone: Isotype: IgG
Applications: ELISA (EIA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB)

(Secondary Antibody)
• 1 mg   $395

Sheep anti Bovine IgG1, Secondary Antibody

(#MBS534748)
• 
Sheep anti Bovine IgG1
Gene Names: IgG1
Other Names: Polyclonal Sheep anti Bovine IgG1; Anti-Sheep anti Bovine IgG1; Bovine Immuonglobulin G1; Cow IgG1
Host/Reactivities: Host: Sheep / Reactivity: Bovine
Purification: Sheep anti Bovine IgG1 antibody was purified by affinity chromatography.
Isotype/Clone: Isotype: IgG
Applications: ELISA (EIA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB)

(Secondary Antibody)
• 1 mg   $280

Sheep anti Bovine IgG1 (HRP), Secondary Antibody

(#MBS539222)
• 
Sheep anti Bovine IgG1 (HRP)
Gene Names: IgG1
Other Names: Bovine Immuonglobulin G2 (HRP); Cow IgG2 (HRP)
Host/Reactivities: Host: Sheep / Reactivity: Bovine
Purification: Sheep anti Bovine IgG1 (HRP) was purified by affinity chromatography.
Isotype/Clone: Isotype: IgG
Applications: ELISA (EIA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB)

(Secondary Antibody)
• 1 mg   $310

Sheep anti Bovine IgG1 (HRP), Secondary Antibody

(#MBS539099)
• 
Sheep anti Bovine IgG1 (HRP)
Gene Names: IgG1
Other Names: Bovine Immuonglobulin G1 (HRP); Cow IgG1 (HRP)
Host/Reactivities: Host: Sheep / Reactivity: Bovine
Purification: Sheep anti Bovine IgG1 (HRP) was purified by affinity chromatography.
Isotype/Clone: Isotype: IgG
Applications: ELISA (EIA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB)

(Secondary Antibody)
• 1 mg   $335

Sheep anti Bovine IgG1 (subclass specific), Polyclonal Secondary Antibody

(#MBS571238)
• 
Sheep anti Bovine IgG1 (subclass specific)
Host/Reactivities: Host: Sheep / Reactivity: Bovine
Applications: Immunoprecipitation (IP)

(Secondary Antibody)
• 1 mL   $300

Sheep anti Bovine IgG2 (subclass specific), Polyclonal Secondary Antibody

(#MBS571723)
• 
Sheep anti Bovine IgG2 (subclass specific)
Host/Reactivities: Host: Sheep / Reactivity: Bovine
Applications: Immunoprecipitation (IP)

(Secondary Antibody)
• 1 mL   $300

Sheep anti Bovine IgM, Secondary Antibody

(#MBS538391)
• 
Sheep anti Bovine IgM
Gene Names: IgM
Host/Reactivities: Host: Sheep / Reactivity: Bovine
Purification: Sheep anti Bovine IgM was purified by affinity chromatography.
Isotype/Clone: Isotype: IgG
Applications: ELISA (EIA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB)

(Secondary Antibody)
• 1 mg   $245

Sheep anti Bovine IgM (HRP), Secondary Antibody

(#MBS539291)
• 
Sheep anti Bovine IgM (HRP)
Gene Names: IgM
Host/Reactivities: Host: Sheep / Reactivity: Bovine
Purification: Sheep anti Bovine IgM (HRP) was purified by affinity chromatography.
Isotype/Clone: Isotype: IgG
Applications: ELISA (EIA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB)

(Secondary Antibody)
• 1 mg   $315

Sheep anti Bovine IgM (HRP), Secondary Antibody

(#MBS539081)
• 
Sheep anti Bovine IgM (HRP)
Gene Names: IgM
Other Names: Bovine Immuonglobulin M (HRP); Cow IgM (HRP)
Host/Reactivities: Host: Sheep / Reactivity: Bovine
Purification: Sheep anti Bovine IgM (HRP) was purified by affinity chromatography.
Isotype/Clone: Isotype: IgG
Applications: ELISA (EIA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB)

(Secondary Antibody)
• 1 mg   $335

Sheep anti Dog IgG (Fc specific), Polyclonal Secondary Antibody

(#MBS571823)
• 
Sheep anti Dog IgG (Fc specific)
Host/Reactivities: Host: Sheep / Reactivity: Canine
Applications: Immunoprecipitation (IP)

(Secondary Antibody)
• 1 mL   $300

Sheep anti Guinea Pig IgA (Fc specific), Polyclonal Secondary Antibody

(#MBS571156)
• 
Sheep anti Guinea Pig IgA (Fc specific)
Host/Reactivities: Host: Sheep / Reactivity: Guinea Pig
Applications: Immunoprecipitation (IP)

(Secondary Antibody)
• 1 mL   $300

Sheep anti Guinea Pig IgG (Fc specific), Polyclonal Secondary Antibody

(#MBS571441)
• 
Sheep anti Guinea Pig IgG (Fc specific), conjugated with FITC
Host/Reactivities: Host: Sheep / Reactivity: Guinea Pig
Applications: ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), (In)direct Immunofluorescence (IF)

(Secondary Antibody)
• 1 mL   $400

Sheep anti Guinea Pig IgG (Fc specific), Polyclonal Secondary Antibody

(#MBS571368)
• 
Sheep anti Guinea Pig IgG (Fc specific), conjugated with Horseradish peroxidase
Host/Reactivities: Host: Sheep / Reactivity: Guinea Pig
Applications: ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), Dot Blot (DB), Immunoblotting (IB)

(Secondary Antibody)
• 1 mL   $510

Sheep anti Guinea Pig IgG (Fc specific), Polyclonal Secondary Antibody

(#MBS571382)
• 
Sheep anti Guinea Pig IgG (Fc specific), conjugated with Biotin
Host/Reactivities: Host: Sheep / Reactivity: Guinea Pig
Applications: ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), Dot Blot (DB), Immunoblotting (IB)

(Secondary Antibody)
• 1 mL   $510

Sheep anti Guinea Pig IgG1 (subclass specific), Polyclonal Secondary Antibody

(#MBS571604)
• 
Sheep anti Guinea Pig IgG1 (subclass specific)
Host/Reactivities: Host: Sheep / Reactivity: Guinea Pig
Applications: Immunoprecipitation (IP)

(Secondary Antibody)
• 1 mL   $300

Sheep anti Guinea Pig IgG1 IgG2 IgA IgM (heavy and light chains), Polyclonal Secondary Antibody

(#MBS571828)
• 
Sheep anti Guinea Pig IgG1 IgG2 IgA IgM (heavy and light chains)
Host/Reactivities: Host: Sheep / Reactivity: Guinea Pig
Applications: Immunoprecipitation (IP)

(Secondary Antibody)
• 1 mL   $300

Sheep anti Guinea Pig IgM (Fc specific), Polyclonal Secondary Antibody

(#MBS571459)
• 
Sheep anti Guinea Pig IgM (Fc specific)
Host/Reactivities: Host: Sheep / Reactivity: Guinea Pig
Applications: Immunoprecipitation (IP)

(Secondary Antibody)
• 1 mL   $300

Sheep anti Guinea Pig Serum proteins, Polyclonal Secondary Antibody

(#MBS571720)
• 
Sheep anti Guinea Pig Serum proteins
Host/Reactivities: Host: Sheep / Reactivity: Guinea Pig
Applications: Immunoprecipitation (IP)

(Secondary Antibody)
• 1 mL   $240

Sheep anti Horse IgG (heavy and light chains), Polyclonal Secondary Antibody

(#MBS571744)
• 
Sheep anti Horse IgG (heavy and light chains)
Host/Reactivities: Host: Sheep / Reactivity: Equine
Applications: Immunoprecipitation (IP)

(Secondary Antibody)
• 1 mL   $240

Sheep anti Human Ig kappa light chains (free and bound), Polyclonal Secondary Antibody

(#MBS571542)
• 
Sheep anti Human Ig kappa light chains (free and bound)
Host/Reactivities: Host: Sheep / Reactivity: Human
Applications: Immunoprecipitation (IP)

(Secondary Antibody)
• 1 mL   $345

Sheep anti Human Ig kappa light chains (free), Polyclonal Secondary Antibody

(#MBS571683)
• 
Sheep anti Human Ig kappa light chains (free)
Host/Reactivities: Host: Sheep / Reactivity: Human
Applications: Immunoprecipitation (IP)

(Secondary Antibody)
• 1 mL   $430

Sheep anti Human Ig lambda light chains (free), Polyclonal Secondary Antibody

(#MBS571165)
• 
Sheep anti Human Ig lambda light chains (free)
Host/Reactivities: Host: Sheep / Reactivity: Human
Applications: Immunoprecipitation (IP)

(Secondary Antibody)
• 1 mL   $430

Sheep anti Human IgA, Secondary Antibody

(#MBS535860)
• 
Sheep anti Human IgA
Gene Names: IgA
Other Names: Polyclonal Human IgA; Anti-Human IgA; Immunoglobulin A; partial
Host/Reactivities: Host: Sheep / Reactivity: Human
Applications: ODD, Immunodiffusion, Western Blot (WB)

(Secondary Antibody)
• 1 mL   $265

Sheep anti Human IgA (Fc specific), Polyclonal Secondary Antibody

(#MBS571902)
• 
Sheep anti Human IgA (Fc specific), conjugated with TRITC
Host/Reactivities: Host: Sheep / Reactivity: Human
Applications: ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), (In)direct Immunofluorescence (IF)

(Secondary Antibody)
• 1 mL   $400

Sheep anti Human IgA1 IgA2 (sublass specific), Polyclonal Secondary Antibody

(#MBS571511)
• 
Sheep anti Human IgA1 IgA2 (sublass specific)

(Secondary Antibody)
• 1 mL   $610

Sheep anti Human IgD (Fc specific), Polyclonal Secondary Antibody

(#MBS571395)
• 
Sheep anti Human IgD (Fc specific)
Host/Reactivities: Host: Sheep / Reactivity: Human
Applications: Immunoprecipitation (IP)

(Secondary Antibody)
• 1 mL   $280

Sheep anti Human IgD (Fc specific), Polyclonal Secondary Antibody

(#MBS571592)
• 
Sheep anti Human IgD (Fc specific), conjugated with TRITC
Host/Reactivities: Host: Sheep / Reactivity: Human
Applications: ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), (In)direct Immunofluorescence (IF)

(Secondary Antibody)
• 1 mL   $400

Sheep anti Human IgD (Fc specific), Polyclonal Secondary Antibody

(#MBS571630)
• 
Sheep anti Human IgD (Fc specific)
Host/Reactivities: Host: Sheep / Reactivity: Human
Applications: Indirect Immunofluorescence (IF), ELISA (EIA), Dot Blot (DB), Immunoblotting (IB)

(Secondary Antibody)
• 10 mg   $330

Sheep anti Human IgD (Fc specific), Polyclonal Secondary Antibody

(#MBS571887)
• 
Sheep anti Human IgD (Fc specific), conjugated with FITC
Host/Reactivities: Host: Sheep / Reactivity: Human
Applications: ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), (In)direct Immunofluorescence (IF)

(Secondary Antibody)
• 1 mL   $400

Sheep anti Human IgE (Fc specific), Polyclonal Secondary Antibody

(#MBS571212)
• 
Sheep anti Human IgE (Fc specific)
Host/Reactivities: Host: Sheep / Reactivity: Human
Applications: Immunoprecipitation (IP)

(Secondary Antibody)
• 1 mL   $280

Sheep anti Human IgG (Fc specific), Polyclonal Secondary Antibody

(#MBS571344)
• 
Sheep anti Human IgG (Fc specific), conjugated with TRITC
Host/Reactivities: Host: Sheep / Reactivity: Human
Applications: ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), (In)direct Immunofluorescence (IF)

(Secondary Antibody)
• 1 mL   $400

Sheep anti Human IgG gamma, Secondary Antibody

(#MBS535924)
• 
Sheep anti Human IgG gamma
Gene Names: IgG gamma
Other Names: Polyclonal Human IgG gamma; Anti-Human IgG gamma; IgG
Host/Reactivities: Host: Sheep / Reactivity: Human
Isotype/Clone: Isotype: IgG
Applications: ODD, Immunodiffusion, Western Blot (WB)

(Secondary Antibody)
• 1 mL   $220

Sheep anti Human IgG1 IgG2 IgG3 IgG4 (subclass specific), Polyclonal Secondary Antibody

(#MBS571433)
• 
Sheep anti Human IgG1 IgG2 IgG3 IgG4 (subclass specific)

(Secondary Antibody)
• 2 mL   $825

Sheep anti Human IgG3 (subclass specific), Polyclonal Secondary Antibody

(#MBS571562)
• 
Sheep anti Human IgG3 (subclass specific)
Host/Reactivities: Host: Sheep / Reactivity: Human
Applications: Precipitation assays

(Secondary Antibody)
• 1 mL   $430

Sheep anti Human IgG4 (subclass specific), Polyclonal Secondary Antibody

(#MBS571304)
• 
Sheep anti Human IgG4 (subclass specific)
Host/Reactivities: Host: Sheep / Reactivity: Human
Applications: Immunoprecipitation (IP)

(Secondary Antibody)
• 1 mL   $430

Sheep anti Human IgM, Secondary Antibody

(#MBS536137)
• 
Sheep anti Human IgM
Gene Names: IgM
Other Names: Polyclonal Human IgM; Anti-Human IgM
Host/Reactivities: Host: Sheep / Reactivity: Human
Isotype/Clone: Isotype: IgM
Applications: ODD, Immunodiffusion, Western Blot (WB)

(Secondary Antibody)
• 1 mL   $280

Sheep anti Human secretory component (free and bound), Polyclonal Secondary Antibody

(#MBS571288)
• 
Sheep anti Human secretory component (free and bound)
Host/Reactivities: Host: Sheep / Reactivity: Human
Applications: Immunoprecipitation (IP)

(Secondary Antibody)
• 1 mL   $300

Sheep anti Human secretory component (free and bound), Polyclonal Secondary Antibody

(#MBS571863)
• 
Sheep anti Human secretory component (free and bound)
Host/Reactivities: Host: Sheep / Reactivity: Human
Applications: Indirect Immunofluorescence (IF), ELISA (EIA), Dot Blot (DB), Immunoblotting (IB)

(Secondary Antibody)
• 10 mg   $330

Sheep anti Human secretory IgA, Polyclonal Secondary Antibody

(#MBS571115)
• 
Sheep anti Human secretory IgA
Host/Reactivities: Host: Sheep / Reactivity: Human
Applications: Immunoprecipitation (IP)

(Secondary Antibody)
• 1 mL   $280

Sheep anti Mouse Ig kappa light chains (free and bound), Polyclonal Secondary Antibody

(#MBS571785)
• 
Sheep anti Mouse Ig kappa light chains (free and bound), conjugated with FITC
Host/Reactivities: Host: Sheep / Reactivity: Mouse
Applications: ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), (In)direct Immunofluorescence (IF)

(Secondary Antibody)
• 1 mL   $510

Sheep anti Mouse Ig kappa light chains (free and bound), Polyclonal Secondary Antibody

(#MBS571574)
• 
Sheep anti Mouse Ig kappa light chains (free and bound)
Host/Reactivities: Host: Sheep / Reactivity: Mouse
Applications: Indirect Immunofluorescence (IF), ELISA (EIA), Dot Blot (DB), Immunoblotting (IB)

(Secondary Antibody)
• 10 mg   $510

Sheep anti Mouse Ig kappa light chains (free and bound), Polyclonal Secondary Antibody

(#MBS571381)
• 
Sheep anti Mouse Ig kappa light chains (free and bound), conjugated with TRITC
Host/Reactivities: Host: Sheep / Reactivity: Mouse
Applications: ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), (In)direct Immunofluorescence (IF)

(Secondary Antibody)
• 1 mL   $510

Sheep anti Mouse Ig lambda light chains (free and bound), Polyclonal Secondary Antibody

(#MBS571674)
• 
Sheep anti Mouse Ig lambda light chains (free and bound), conjugated with Horseradish peroxidase
Host/Reactivities: Host: Sheep / Reactivity: Mouse
Applications: ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), Dot Blot (DB), Immunoblotting (IB)

(Secondary Antibody)
• 1 mL   $510

Sheep anti Mouse Ig lambda light chains (free and bound), Polyclonal Secondary Antibody

(#MBS571182)
• 
Sheep anti Mouse Ig lambda light chains (free and bound), conjugated with TRITC
Host/Reactivities: Host: Sheep / Reactivity: Mouse
Applications: ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), (In)direct Immunofluorescence (IF)

(Secondary Antibody)
• 1 mL   $510

Sheep anti Mouse Ig lambda light chains (free and bound), Polyclonal Secondary Antibody

(#MBS571523)
• 
Sheep anti Mouse Ig lambda light chains (free and bound)
Host/Reactivities: Host: Sheep / Reactivity: Mouse
Applications: Immunoprecipitation (IP)

(Secondary Antibody)
• 1 mL   $345
Displaying 1 to 50 (of 152 products) Result Pages:  1  2  3  4  [Next >>] 
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