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PROK biochemical enzyme :: Proteinase K (PROK) Biochemical Enzyme

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Catalog # MBS7053447
Unit / Price
  0.1 mg  /  $85 +1 FREE 8GB USB
  0.2 mg  /  $110 +1 FREE 8GB USB
  0.5 mg  /  $180 +1 FREE 8GB USB
  1 mg  /  $275 +1 FREE 8GB USB
  2 mg  /  $525 +1 FREE 8GB USB
  5 mg  /  $1,265 +1 FREE 8GB USB
Product Name

Proteinase K (PROK), Biochemical Enzyme

Popular Item
Also Known As

Proteinase K (PROK) Recombinant Protein

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
Full length expression
Sequence
MRLSVLLSLL PLALGAPAVE QRSEAAPLIE ARGEMVANKY IVKFKEGSAL SALDAAMEKI SGKPDHVYKN VFSGFAATLD ENMVRVLRAH PDVEYIEQDA VVTINAAQTN APWGLARISS TSPGTSTYYY DESAGQGSCV YVIDTGIEAS HPEFEGRAQM VKTYYYSSRD GNGHGTHCAG TVGSRTYGVA KKTQLFGVKV LDDNGSGQYS TIIAGMDFVA SDKNNRNCPK GVVASLSLGG GYSSSVNSAA ARLQSSGVMV AVAAGNNNAD ARNYSPASEP SVCTVGASDR YDRRSSFSNY GSVLDIFGPG TSILSTWIGG STRSISGTSM ATPHVAGLAA YLMTLGKTTA ASACRYIADT ANKGDLSNIP FGTVNLLAYN NYQA
3D Structure
ModBase 3D Structure for P06873
Host
Yeast
Purity/Purification
>=95% SDS-PAGE
Form/Format
Lyophilized powder
Specific Activity
>=30U/mg
Gene
Tritirachium album Liber
Product Note
Applications are user defined. Product is developed and quality control tested in house, data is not provided. The researcher needs to establish and confirm the suitability of the product for their application.
Tag type
His Tag
Tag Position
C-terminal
Preparation and Storage
Store at -20 or -80 degree C.
Avoid repeated freeze/thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of PROK biochemical enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PROK biochemical enzyme
Proteinase K is an extracellular serine endoproteinase produced by Tritirachium album Limber. Proteinase K belongs to the peptidase family S8. Its predominant cleavage site is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked ?-amino groups. Proteinase K is a broad-spectrum protease that functions at wide pH and temperature ranges. The optimum pH and temperature for the activity of this protease were approximately pH 7.5-8.0 and 55°C, respectively. In addition, 0.2-1% sodium dodecyl sulfate (SDS) and 10mM carbamide can activate its activity.

The combination of these unique features makes Proteinase K as an important tool in scientific research. Proteinase K can be used in the preparation of biological macromolecules by degrading the constitutive proteins of cells and removing nucleases such as DNases and RNases for RT-PCR. In nucleic acid vaccine and molecular diagnosis, it is also widely used for degrading proteinaceous impurities during the isolation of nucleic acids from biological samples.

The latest launch of high quality of Proteinase K shows its high purity and high enzyme activity by biochemical platform testing. Through repeated inspection, all the data show our company produced Proteinase K without RNA, DNA, RNAse and DNAse residues.
Applications Tested/Suitable for PROK biochemical enzyme
Raw Materials for Genomic DNA, Raw Materials for RNA Purification, RNA purification kits, IHC, ISH and other

Calibration Curve of PROK biochemical enzyme
According to the absorbance A275 for the vertical axis, the different concentrations of tyrosine as the horizontal axis, the standard curve was drawn. Then, the enzyme activity was 30U/mg by calculating (Fig.1).
Unit definition: One unit is defined as the amount of enzyme that catalyzes the formation of 1 umol of tyrosine per minute at pH 7.5 at 37°C
PROK biochemical enzyme Calibration Curve image
Protein Purity of PROK biochemical enzyme
The purity and the molecular weight of MBS7053447 proteinase K were over 95% and 28.9 kDa by SDS-PAGE, respectively (Fig.2).
PROK biochemical enzyme  Protein Purity image
Detection of Nucleic Acid Residue of PROK biochemical enzyme
By agarose gel electrophoresis to detect nucleic acid, figure 3 shows MBS7053447produced proteinase K without RNA, DNA residues (Fig.3).
PROK biochemical enzyme  Detection of Nucleic Acid Residue image
Detection of DNAse Residue of PROK biochemical enzyme
DNAses remained in lyophilized powder were detected by agarose gel electrophoresis , figure 4 shows MBS7053447 produced proteinase K without DNAse residue after 6h of incubation at 37°C (Fig.4).
PROK biochemical enzyme  Detection of DNAse Residue image
Detection of RNAse Residue of PROK biochemical enzyme
Agarose gel electrophoresis was performed to check whether there was residual RNase. Figure 5 shows MBS7053447 produced proteinase K without RNAse residue after 16h of incubated at 25°C (Fig.5).
PROK biochemical enzyme  Detection of RNAse Residue image
NCBI/Uniprot data below describe general gene information for PROK. It may not necessarily be applicable to this product.
UniProt Primary Accession #
Molecular Weight
65,531 Da
NCBI Official Full Name
proteinase K
UniProt Protein Name
Proteinase K
UniProt Synonym Protein Names
Proteinase K
Protein Family
UniProt Gene Name
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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