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PROK1 elisa kit :: Human prokineticin 1 ELISA Kit

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Catalog # MBS703707
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $435 +1 FREE 8GB USB
  96-Strip-Wells  /  $625 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,595 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,905 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

prokineticin 1 (PROK1), ELISA Kit

Full Product Name

Human prokineticin 1, PROK1/EG-VEGF ELISA Kit

Product Synonym Names
Human prokineticin 1 (PROK1/EG-VEGF) ELISA kit; EGVEGF; PK1; PRK1; black mamba toxin-related protein; mambakine; Endocrine gland vascular endothelial growth factor; prokineticin 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS703707 Testing Data
OMIM
606233
3D Structure
ModBase 3D Structure for P58294
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human PROK1. No significant cross-reactivity or interference between Human PROK1 and analogues was observed.
Samples
Serum
Detection Range
0.312 ng/ml-20 ng/ml
Sensitivity
0.078 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Angiogenesis
Protein Biological Process 3
Angiogenesis
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PROK1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPROK1purchase
MBS703707 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the prokineticin 1 (PROK1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PROK1. The ELISA analytical biochemical technique of the MBS703707 kit is based on PROK1 antibody-PROK1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PROK1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PROK1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PROK1 elisa kit
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to EG-VEGF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for EG-VEGF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain EG-VEGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of EG-VEGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of PROK1 elisa kit
PROK1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS703707. Click to request current manual
NCBI/Uniprot data below describe general gene information for PROK1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
11,715 Da
NCBI Official Full Name
prokineticin-1
NCBI Official Synonym Full Names
prokineticin 1
NCBI Official Symbol
PROK1  [Similar Products]
NCBI Official Synonym Symbols
PK1; PRK1; EGVEGF
  [Similar Products]
NCBI Protein Information
prokineticin-1; EG-VEGF; mambakine; black mamba toxin-related protein; endocrine-gland-derived vascular endothelial growth factor
UniProt Protein Name
Prokineticin-1
UniProt Synonym Protein Names
Endocrine-gland-derived vascular endothelial growth factor; EG-VEGF; Mambakine
Protein Family
UniProt Gene Name
PROK1  [Similar Products]
UniProt Synonym Gene Names
UNQ600/PRO1186; EG-VEGF  [Similar Products]
UniProt Entry Name
PROK1_HUMAN
NCBI Summary for PROK1
The protein encoded by this gene induces proliferation, migration, and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It has little or no effect on a variety of other endothelial and non-endothelial cell types. Its expression is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta), is induced by hypoxia, and often complementary to the expression of vascular endothelial growth factor (VEGF), suggesting that these molecules function in a coordinated manner. [provided by RefSeq, Sep 2011]
UniProt Comments for PROK1
Function: Potently contracts gastrointestinal (GI) smooth muscle. Induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. Has little or no effect on a variety of other endothelial and non-endothelial cell types. Induces proliferation and differentiation, but not migration, of enteric neural crest cells. Directly influences neuroblastoma progression by promoting the proliferation and migration of neuroblastoma cells. Positively regulates PTGS2 expression and prostaglandin synthesis. May play a role in placentation. May play a role in normal and pathological testis angiogenesis.5 PublicationsManual assertion based on experiment in:Ref.1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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