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PROK2 elisa kit :: Human prokineticin 2 ELISA Kit

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Catalog # MBS940962
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

prokineticin 2 (PROK2), ELISA Kit

Popular Item
Also Known As

Human Prokineticin-2, PROK2 ELISA Kit

Product Synonym Names
Human Prokineticin-2 (PROK2) ELISA kit; BV8; KAL4; MIT1; PK2; protein Bv8 homolog; prokineticin 2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS940962 Testing Data
Sequence Length
129
OMIM
gene 610628
3D Structure
ModBase 3D Structure for Q9HC23
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human PROK2. No significant cross-reactivity or interference between human PROK2 and analogues was observed.
Samples
Serum, plasma, cell culture supernates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
6.25 pg/ml-400 pg/ml
Sensitivity
<1.56 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PROK2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPROK2purchase
MBS940962 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the prokineticin 2 (PROK2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PROK2. The ELISA analytical biochemical technique of the MBS940962 kit is based on PROK2 antibody-PROK2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PROK2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PROK2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PROK2 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PROK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PROK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PROK2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PROK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of PROK2 elisa kit
PROK2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS940962. Click to request current manual
NCBI/Uniprot data below describe general gene information for PROK2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
14,314 Da
NCBI Official Full Name
prokineticin-2 isoform a
NCBI Official Synonym Full Names
prokineticin 2
NCBI Official Symbol
PROK2  [Similar Products]
NCBI Official Synonym Symbols
BV8; HH4; PK2; KAL4; MIT1
  [Similar Products]
NCBI Protein Information
prokineticin-2; protein Bv8 homolog
UniProt Protein Name
Prokineticin-2
UniProt Synonym Protein Names
Protein Bv8 homolog
Protein Family
UniProt Gene Name
PROK2  [Similar Products]
UniProt Synonym Gene Names
BV8; PK2  [Similar Products]
UniProt Entry Name
PROK2_HUMAN
NCBI Summary for PROK2
This gene encodes a protein expressed in the suprachiasmatic nucleus (SCN) circadian clock that may function as the output component of the circadian clock. The secreted form of the encoded protein may also serve as a chemoattractant for neuronal precursor cells in the olfactory bulb. Proteins from other vertebrates which are similar to this gene product were isolated based on homology to snake venom and secretions from frog skin, and have been shown to have diverse functions. Mutations in this gene are associated with Kallmann syndrome 4. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for PROK2
PROK2: May function as an output molecule from the suprachiasmatic nucleus (SCN) that transmits behavioral circadian rhythm. May also function locally within the SCN to synchronize output. Potently contracts gastrointestinal (GI) smooth muscle. Defects in PROK2 are the cause of Kallmann syndrome type 4 (KAL4); also known as hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin- releasing hormone-synthesizing neurons. KAL4 patients have variable degrees of olfactory and reproductive dysfunction, but do not show any of the occasional clinical anomalies reported in Kallmann syndrome such as renal agenesis, cleft lip/palate, selective tooth agenesis, and bimanual synkinesis. Belongs to the AVIT (prokineticin) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3p13

Cellular Component: extracellular region

Molecular Function: G-protein-coupled receptor binding

Biological Process: circadian rhythm; activation of MAPK activity; sensory perception of pain; chemotaxis; cell proliferation; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; neuropeptide signaling pathway; spermatogenesis; angiogenesis; positive regulation of smooth muscle contraction; inflammatory response; negative regulation of apoptosis

Disease: Hypogonadotropic Hypogonadism 4 With Or Without Anosmia
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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