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Protein C elisa kit :: Rabbit Protein C ELISA Kit

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Catalog # MBS723552
Unit / Price
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  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 Typical Testing Data/Standard Curve (for reference only)
Product Name

Protein C, ELISA Kit

Popular Item
Full Product Name

Rabbit Protein C ELISA Kit

Product Synonym Names
Rabbit Protein C (Protein C) ELISA Kit; Rabbit Protein C ELISA Kit; Protein C; Protein C (Rabbit)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Chromosome Location
Chromosome: 2; NC_000002.11 (128175996..128186822). Location: 2q13-q14
OMIM
176860
3D Structure
ModBase 3D Structure for P04070
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of PROC. No significant cross-reactivity or interference between PROC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PROC and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Sandwich
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Protein C elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for Protein Cpurchase
MBS723552 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Protein C, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Protein C. The ELISA analytical biochemical technique of the MBS723552 kit is based on Protein C antibody-Protein C antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Protein C antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Protein C. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
Protein C elisa kit
Intended Uses: This PROC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit PROC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: PROC ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PROC. Standards or samples are then added to the microtiter plate wells and PROC if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PROC present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PROC are added to each well to "sandwich" the PROC immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PROC and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PROC concentration in each sample is interpolated from this standard curve.
Product Categories/Family for Protein C elisa kit

Typical Testing Data/Standard Curve (for reference only) of Protein C elisa kit
Protein C elisa kit  Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS723552. Click to request current manual
NCBI/Uniprot data below describe general gene information for Protein C. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
52,071 Da
NCBI Official Full Name
protein C
NCBI Official Synonym Full Names
protein C (inactivator of coagulation factors Va and VIIIa)
NCBI Official Symbol
NCBI Official Synonym Symbols
PC; APC; PROC1
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NCBI Protein Information
vitamin K-dependent protein C; OTTHUMP00000162263; OTTHUMP00000203820; OTTHUMP00000203827; autoprothrombin IIA; anticoagulant protein C; blood coagulation factor XIV
UniProt Protein Name
Vitamin K-dependent protein C
UniProt Synonym Protein Names
Anticoagulant protein C; Autoprothrombin IIA; Blood coagulation factor XIV
Protein Family
UniProt Gene Name
UniProt Entry Name
PROC_HUMAN
NCBI Summary for Protein C
This gene encodes a vitamin K-dependent plasma glycoprotein. The encoded protein is cleaved to its activated form by the thrombin-thrombomodulin complex. This activated form contains a serine protease domain and functions in degradation of the activated forms of coagulation factors V and VIII. Mutations in this gene have been associated with thrombophilia due to protein C deficiency, neonatal purpura fulminans, and recurrent venous thrombosis.
UniProt Comments for Protein C
Function: Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids.

Catalytic activity: Degradation of blood coagulation factors Va and VIIIa.

Subunit structure: Synthesized as a single chain precursor, which is cleaved into a light chain and a heavy chain held together by a disulfide bond. The enzyme is then activated by thrombin, which cleaves a tetradecapeptide from the amino end of the heavy chain; this reaction, which occurs at the surface of endothelial cells, is strongly promoted by thrombomodulin.

Tissue specificity: Plasma; synthesized in the liver.

Post-translational modification: The vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium.Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not glycosylated at Asn-371, is beta. Ref.12 Ref.14The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.

Involvement in disease: Defects in PROC are the cause of protein C deficiency autosomal dominant (ADPROCD) [

MIM:176860]. ADPROCD is a cause of hereditary thrombophilia, a hemostatic disorder characterized by impaired regulation of blood coagulation and a tendency to recurrent venous thrombosis. However, many adults with heterozygous disease may be asymptomatic. Individuals with decreased amounts of protein C are classically referred to as having type I protein C deficiency and those with normal amounts of a functionally defective protein as having type II deficiency. Ref.18 Ref.19 Ref.20 Ref.21 Ref.24 Ref.25 Ref.29 Ref.30 Ref.31 Ref.35 Ref.38 Ref.39 Ref.40Defects in PROC are the cause of protein C deficiency autosomal recessive (ARPROCD) [

MIM:612304]. ARPROCD results in a thrombotic condition that can manifest as a severe neonatal disorder or as a milder disorder with late-onset thrombophilia. The severe form leads to neonatal death through massive neonatal venous thrombosis. Often associated with ecchymotic skin lesions which can turn necrotic called purpura fulminans, this disorder is very rare.

Miscellaneous: Calcium also binds, with stronger affinity to another site, beyond the GLA domain. This GLA-independent binding site is necessary for the recognition of the thrombin-thrombomodulin complex.

Sequence similarities: Belongs to the peptidase S1 family.Contains 2 EGF-like domains.Contains 1 Gla (gamma-carboxy-glutamate) domain.Contains 1 peptidase S1 domain.

Sequence caution: The sequence S76088 differs from that shown. Reason: Erroneous termination at position 151. Translated as Cys.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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