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HRP Conjugated Streptavidin Protein

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Catalog # MBS176633
Unit / Price
  0.5 mL  /  $160 +1 FREE 8GB USB
  1 mL  /  $230 +1 FREE 8GB USB
HRP Conjugated Streptavidin protein
Product Name

HRP Conjugated Streptavidin, Protein

Popular Item
Also Known As

HRP Conjugated Streptavidin

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Form/Format
Concentrated, Liquid
Concentration
1mg/ml (lot specific)
Contents
1 mg of peroxidase conjugated streptavidin; 0.01M PBS (pH 7.4), 50% glycerol
Labeling Method
Streptavidin is conjugated to peroxidase by means of a method described by Wilson MB and Nakane PK
Preparation of Dilutent Buffer
Use 0.01 M TBS or 0.01 M PBS to dilute:

Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450?l of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g Na2HPO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation and Storage
At 4 degree C for one year.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of HRP Conjugated Streptavidin protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
HRP Conjugated Streptavidin protein
Description: Streptavidin is a 47KD protein that extracted from streptomyces. Just like avidin, streptavidin has very high affinity to biotin molecule, one million times than the common affinity between antigen and antibody. Avidin is a alkalic protein (IP=10.0-10.5), and it can transfer to be a neutral protein through reconstruction. Isoelectric point of the streptavidin is near to neutrality, IP=6.0~6.5. Thus, streptavidin has very low non-specific absorption to tissue and cell. On the basis of streptavidin, the background of immunohistochemistry analysis is extremely low. With the method of polymeric labeling, streptavidin-HRP can form a complex which composed by about one hundred HRP and fifty streptavidin. And lots of enzymes can ensure the streptavidin-HRP with high sensitivity.
Applications Tested/Suitable for HRP Conjugated Streptavidin protein
Dot Blot (DB), Western Blot (WB), ELISA (EIA)
Dot blot(ECM): 0.25-0.5ug/ml
Western blot(DAB): 0.3-2ug/ml
Western blot(ECM): 0.1-0.3ug/ml
ELISA: 0.025-0.05ug/ml

Optimal working dilutions should be determined be end users.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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