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Sporulation killing factor maturation protein

Catalyzes the formation of the thioether bond required for production of the sporulation killing factor (SKF) from SkfA .

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Sporulation killing factor maturation protein SkfB

Catalyzes the formation of the thioether bond required for production of the sporulation killing factor (SKF) from SkfA (PubMed:12817086). Forms the cysteine-methionine thioether bond found in SKF; the acceptor amino acid can be hydrophobic, aromatic or a small hydrophilic amino acid but not a larger hydrophilic amino acid, i.e. Met=Ala, Phe, Leu, Tyr>Asn, Ser>>Gln, Glu, Lys (PubMed:23282011). The relative position of Cys and Met in the substrate cannot be inverted, in vitro the thioether bond cannot be made in the absence of the SkfA propeptide, suggesting this is the first reaction in SKF maturation (PubMed:23282011). In vitro, in the absence of a second substrate, cleaves S-adenosyl-L-methionine into Met and 5'-dA (PubMed:23282011).

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