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PSA elisa kit :: Human PSA ELISA Kit

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Catalog # MBS494521
Unit / Price DISCONTINUED
PSA elisa kit
Product Name

PSA, ELISA Kit

Popular Item
Also Known As

PSA (Total) ELISA

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
261
OMIM
176820
3D Structure
ModBase 3D Structure for P07288
Species Reactivity
Samples
Serum and plasma
Preparation and Storage
2 - 8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PSA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPSApurchase
MBS494521 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PSA, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PSA. The ELISA analytical biochemical technique of the MBS494521 kit is based on PSA antibody-PSA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PSA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PSA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PSA elisa kit
Intended Uses||The Measles IgG Antibody ELISA Test Kit has been designed for the detection and the quantitative determination of specific IgG antibodies against Measles in serum and plasma. For in-vitro diagnostic use

Principle of the Assay: The Measles IgG antibody test kit is based on the principle of the enzyme immunoassay (EIA). Measles antigen is bound on the surface of the microtiter strips. Diluted patient serum or ready-to-use standards are pipetted into the wells of the microtiter plate. A binding between the IgG antibodies of the serum and the immobilized Measles antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti-human-lgG peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the evelopment of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of the IgG antibodies is directly proportional to the intensity of the color.
Sample Manual Insert of MBS494521. Click to request current manual
NCBI/Uniprot data below describe general gene information for PSA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
25,116 Da
NCBI Official Full Name
prostate-specific antigen isoform 1 preproprotein
NCBI Official Synonym Full Names
kallikrein-related peptidase 3
NCBI Official Symbol
NCBI Official Synonym Symbols
APS; PSA; hK3; KLK2A1
  [Similar Products]
NCBI Protein Information
prostate-specific antigen; P-30 antigen; gamma-seminoprotein; kallikrein-3; prostate specific antigen; semenogelase; seminin
UniProt Protein Name
Prostate-specific antigen
UniProt Synonym Protein Names
Gamma-seminoprotein; Seminin; Kallikrein-3; P-30 antigen; Semenogelase
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
APS; PSA; Seminin  [Similar Products]
UniProt Entry Name
KLK3_HUMAN
NCBI Summary for PSA
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Comments for PSA
PSA: Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. Forms heterodimer with SERPINA5. Inhibited by SERPINA5. Activity is strongly inhibited by Zn2+, 100 times more abundant in semen than in serum. This inhibition is relieved by exposure to semenogelins, which are avid zinc binders. Belongs to the peptidase S1 family. Kallikrein subfamily.

Protein type: Secreted; Secreted, signal peptide; Protease; EC 3.4.21.77

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: extracellular region; nucleus

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: negative regulation of angiogenesis; cellular protein metabolic process; proteolysis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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