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PTCH1 recombinant protein :: Patched 1 (PTCH1) Recombinant Protein

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Catalog # MBS2011579 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $140 +1 FREE 8GB USB
  0.05 mg  /  $265 +1 FREE 8GB USB
  0.1 mg  /  $405 +1 FREE 8GB USB
  0.2 mg  /  $500 +1 FREE 8GB USB
  0.5 mg  /  $970 +1 FREE 8GB USB
  1 mg  /  $1,435 +1 FREE 8GB USB
SDS-Page
Product Name

Patched 1 (PTCH1), Recombinant Protein

Popular Item
Also Known As

Recombinant Patched 1 (PTCH1)

Matching Pairs
Matching Pairs
APC-CY7 Conjugated Antibody: Patched 1 (PTCH1) (MBS2082228)
Immunogen: Patched 1 (PTCH1) (MBS2011579)
Matching Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
1434
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- LIITPLDC FWEGAKLQSG TAYLLGKPPL RWTNFDPLEF LEELKKINYQ VDSWEEMLNK AEVGHGYMDR PCLNPADPDC PATAPNKNST KPLDVALVLN GGCQGLSRKY MHWQEELIVG GTVKNATGKL VSAHALQTMF QLMTPKQMYE HFRGYDYVSH INWNEDRAAA ILEAWQRTYV EVVHQSVAPN STQKVLPFTT TTLDDILKSF SD
Chromosome Location
Chromosome: 13; NC_000079.6 (63508328..63573460, complement). Location: 13 B3; 13 32.8 cM
3D Structure
ModBase 3D Structure for Q61115
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in 20mM Tris, 500mM NaCI, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
Organism
Mus musculus (Mouse)
Expression System
Prokaryotic expression
Residues
Leu213~Asp422 (Accession # Q61115) with two N-terminal Tags, His-tag and T7-tag
Usage
Reconstitute in ddH2O
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Predicted Isoelectric Point
6.3
Subcellular Location
Membrane; Multi-pass membrane protein.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Other Notes
Small volumes of PTCH1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for PTCH1 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

NCBI/Uniprot data below describe general gene information for PTCH1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
27.3kDa
NCBI Official Full Name
protein patched homolog 1
NCBI Official Synonym Full Names
patched homolog 1
NCBI Official Symbol
Ptch1  [Similar Products]
NCBI Official Synonym Symbols
Ptc; mes; Ptc1; Ptch; A230106A15Rik
  [Similar Products]
NCBI Protein Information
protein patched homolog 1; Patched 1; Ptch1 protein +12b; mesenchymal dysplasia
UniProt Protein Name
Protein patched homolog 1
UniProt Gene Name
Ptch1  [Similar Products]
UniProt Synonym Gene Names
Ptch; PTC; PTC1  [Similar Products]
UniProt Entry Name
PTC1_MOUSE
UniProt Comments for PTCH1
PTCH1: a multi-pass membrane protein member of the ?patched? family that acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog protein?s signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. Interacts with SNX17. Expressed in tumor cells but not in normal skin. In the embryo, found in all major target tissues of sonic hedgehog, such as the ventral neural tube, somites, and tissues surrounding the zone of polarizing activity of the limb bud. Defects in PTCH1 are the cause of basal cell nevus syndrome (BCNS), also known as Gorlin syndrome. BCNS is an autosomal dominant disease characterized by nevoid basal cell carcinomas and developmental abnormalities such as rib and craniofacial alterations, polydactyly, syndactyly, and spina bifida. In addition, the patients suffer from a multitude of tumors like basal cell carcinomas, fibromas of the ovaries and heart, cysts of the skin, jaws and mesentery, as well as medulloblastomas and meningiomas. PTCH1 defects is also the cause of holoprosencephaly, the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres.

Protein type: Tumor suppressor; Membrane protein, multi-pass; Membrane protein, integral; Cell cycle regulation

Cellular Component: Golgi apparatus; membrane; intracellular membrane-bound organelle; integral to plasma membrane; perinuclear region of cytoplasm; postsynaptic density; extracellular region; integral to membrane; plasma membrane; caveola; midbody

Molecular Function: heparin binding; protein binding; cyclin binding; zinc ion binding; hedgehog receptor activity; cholesterol binding; protein complex binding; patched binding; smoothened binding

Biological Process: heart morphogenesis; hindlimb morphogenesis; epidermis development; regulation of mitotic cell cycle; negative regulation of transcription from RNA polymerase II promoter; cell fate determination; glucose homeostasis; signal transduction; response to chlorate; negative regulation of cell proliferation; regulation of protein localization; mammary gland development; regulation of growth; negative regulation of osteoblast differentiation; dorsoventral neural tube patterning; negative regulation of epithelial cell proliferation; embryonic limb morphogenesis; smoothened signaling pathway; epidermal cell fate specification; negative regulation of multicellular organism growth; pharyngeal system development; in utero embryonic development; negative regulation of transcription factor activity; neural tube patterning; negative regulation of cell division; keratinocyte proliferation; spinal cord motor neuron differentiation; limb morphogenesis; pattern specification process; regulation of cell proliferation; organ morphogenesis; dorsal/ventral pattern formation; ureteric bud branching; neural plate pattern formation; negative regulation of smoothened signaling pathway; positive regulation of epidermal cell differentiation; neural tube closure; embryonic organ development; protein processing; brain development; neural tube formation; regulation of smoothened signaling pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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