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PTGDS elisa kit :: Rat prostaglandin D2 synthase 21kDa (brain) ELISA Kit

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Catalog # MBS917167
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
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Typical Testing Data/Standard Curve (for reference only)
Product Name

prostaglandin D2 synthase 21kDa (brain) (PTGDS), ELISA Kit

Popular Item
Also Known As

Rat Prostaglandin-H2 D-isomerase, PTGDS ELISA Kit

Product Synonym Names
Rat Prostaglandin-H2 D-isomerase (PTGDS) ELISA kit; RP11-229P13.6; L-PGDS; LPGDS; PDS; PGD2; PGDS; PGDS2; PGD2 synthase; beta-trace protein; glutathione-independent PGD synthase; lipocalin-type prostaglandin D synthase; prostaglandin D synthase; prostagla; prostaglandin D2 synthase 21kDa (brain)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS917167 Testing Data
Sequence Length
189
3D Structure
ModBase 3D Structure for P22057
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of rat PTGDS. No significant cross-reactivity or interference between rat PTGDS and analogues was observed.
Samples
Serum, plasma, cerebrospinal fluid (CSF), tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
9.37 ng/ml -600 ng/ml
Sensitivity
Typically less than 2.34 ng/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PTGDS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPTGDSpurchase
MBS917167 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the prostaglandin D2 synthase 21kDa (brain) (PTGDS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PTGDS. The ELISA analytical biochemical technique of the MBS917167 kit is based on PTGDS antibody-PTGDS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PTGDS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PTGDS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PTGDS elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PTGDS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PTGDS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTGDS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTGDS bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of PTGDS elisa kit
PTGDS elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS917167. Click to request current manual
NCBI/Uniprot data below describe general gene information for PTGDS. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
21,301 Da
NCBI Official Full Name
prostaglandin-H2 D-isomerase
NCBI Official Synonym Full Names
prostaglandin D2 synthase (brain)
NCBI Official Symbol
Ptgds  [Similar Products]
NCBI Official Synonym Symbols
PH2DISO
  [Similar Products]
NCBI Protein Information
prostaglandin-H2 D-isomerase; PGDS; PGDS2; PGD2 synthase; Prostaglandin D synthase; prostaglandin-D2 synthase; glutathione-independent PGD synthase; glutathione-independent PGD synthetase; lipocalin-type prostaglandin-D synthase
UniProt Protein Name
Prostaglandin-H2 D-isomerase
UniProt Synonym Protein Names
Glutathione-independent PGD synthase; Lipocalin-type prostaglandin-D synthase; Prostaglandin-D2 synthase; PGD2 synthase; PGDS; PGDS2
UniProt Gene Name
Ptgds  [Similar Products]
UniProt Synonym Gene Names
PGD2 synthase; PGDS; PGDS2  [Similar Products]
UniProt Entry Name
PTGDS_RAT
NCBI Summary for PTGDS
catalyzes conversion of prostaglandin H2 to prostaglandin D2, a major prostaglandin that mediates sleep, body temperature, hormone release, and odor responses [RGD, Feb 2006]
UniProt Comments for PTGDS
Function: Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system.4 PublicationsManual assertion based on experiment in:Ref.8

Catalytic activity: (5Z,13E,15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E,15S)-9-alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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