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PTGES elisa kit :: Mouse Prostaglandin E Synthase, Microsomal ELISA Kit

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Catalog # MBS726011
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Prostaglandin E Synthase, Microsomal (PTGES), ELISA Kit

Popular Item
Full Product Name

Mouse Prostaglandin E Synthase, Microsomal ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
605172
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of PGEsm. No significant cross-reactivity or interference between PGEsm and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PGEsm and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
0.5-10ng/mL
Sensitivity
0.1ng/mL
Intended Uses
This PGEsm ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PGEsm. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PTGES elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPTGESpurchase
MBS726011 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Prostaglandin E Synthase, Microsomal (PTGES) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PTGES. The ELISA analytical biochemical technique of the MBS726011 kit is based on PTGES antibody-PTGES antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PTGES antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PTGES. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PTGES elisa kit
Principle of the Assay: PGEsm ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PGEsm antibody and an PGEsm-HRP conjugate. The assay sample and buffer are incubated together with PGEsm-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PGEsm concentration since PGEsm from samples and PGEsm-HRP conjugate compete for the anti-PGEsm antibody binding site. Since the number of sites is limited, as more sites are occupied by PGEsm from the sample, fewer sites are left to bind PGEsm-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGEsm concentration in each sample is interpolated from this standard curve.

Typical Testing Data/Standard Curve (for reference only) of PTGES elisa kit
PTGES elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS726011. Click to request current manual
NCBI/Uniprot data below describe general gene information for PTGES. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
17,102 Da
NCBI Official Full Name
prostaglandin E synthase
NCBI Official Synonym Full Names
prostaglandin E synthase
NCBI Official Symbol
PTGES  [Similar Products]
NCBI Official Synonym Symbols
PGES; MPGES; PIG12; PP102; PP1294; MGST-IV; MGST1L1; TP53I12; mPGES-1; MGST1-L1
  [Similar Products]
NCBI Protein Information
prostaglandin E synthase; MGST1-like 1; p53-induced gene 12 protein; p53-induced apoptosis protein 12; glutathione S-transferase 1-like 1; microsomal prostaglandin E synthase 1; microsomal prostaglandin E synthase-1; tumor protein p53 inducible protein 12; microsomal glutathione S-transferase 1-like 1
UniProt Protein Name
Prostaglandin E synthase
UniProt Synonym Protein Names
Microsomal glutathione S-transferase 1-like 1; MGST1-L1; Microsomal prostaglandin E synthase 1; MPGES-1; p53-induced gene 12 protein
Protein Family
UniProt Gene Name
PTGES  [Similar Products]
UniProt Synonym Gene Names
MGST1L1; MPGES1; PGES; PIG12; MGST1-L1; MPGES-1  [Similar Products]
UniProt Entry Name
PTGES_HUMAN
NCBI Summary for PTGES
The protein encoded by this gene is a glutathione-dependent prostaglandin E synthase. The expression of this gene has been shown to be induced by proinflammatory cytokine interleukin 1 beta (IL1B). Its expression can also be induced by tumor suppressor protein TP53, and may be involved in TP53 induced apoptosis. Knockout studies in mice suggest that this gene may contribute to the pathogenesis of collagen-induced arthritis and mediate acute pain during inflammatory responses. [provided by RefSeq, Jul 2008]
UniProt Comments for PTGES
PTGES: Catalyzes the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). Belongs to the MAPEG family.

Protein type: Lipid Metabolism - arachidonic acid; Nuclear envelope; Membrane protein, integral; Isomerase; EC 5.3.99.3; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: endoplasmic reticulum membrane; membrane; perinuclear region of cytoplasm; nuclear envelope lumen; integral to membrane

Molecular Function: glutathione binding; prostaglandin-E synthase activity

Biological Process: negative regulation of cell proliferation; chronic inflammatory response; response to retinoic acid; response to cytokine stimulus; cyclooxygenase pathway; arachidonic acid metabolic process; response to lipopolysaccharide; signal transduction; prostaglandin biosynthetic process; response to calcium ion; response to organic cyclic substance; acute inflammatory response; prostaglandin metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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