• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

PTPLA elisa kit :: Human protein tyrosine phosphatase-like (proline instead of catalytic arginine) , member A ELISA Kit

Scan QR to view Datasheet
Catalog # MBS914022
Unit / Price DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Typical Testing Data/Standard Curve (for reference only)
Product Name

protein tyrosine phosphatase-like (proline instead of catalytic arginine) , member A (PTPLA), ELISA Kit

Popular Item
Also Known As

Human 3-hydroxyacyl-CoA dehydratase 1, PTPLA ELISA Kit

Product Synonym Names
Human 3-hydroxyacyl-CoA dehydratase 1 (PTPLA) ELISA kit; CAP; cementum attachment protein; protein tyrosine phosphatase-like; member A; protein-tyrosine phosphatase-like member A; protein tyrosine phosphatase-like (proline instead of catalytic arginine); member A
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS914022 Testing Data
Sequence Length
288
3D Structure
ModBase 3D Structure for B0YJ81
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human PTPLA. No significant cross-reactivity or interference between human PTPLA and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
23.44 pg/ml-1500 pg/ml.
Sensitivity
The minimum detectable dose of human PTPLA is typically less than 5.86 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PTPLA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPTPLApurchase
MBS914022 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the protein tyrosine phosphatase-like (proline instead of catalytic arginine), member A (PTPLA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PTPLA. The ELISA analytical biochemical technique of the MBS914022 kit is based on PTPLA antibody-PTPLA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PTPLA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PTPLA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PTPLA elisa kit
Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PTPLA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PTPLA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTPLA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTPLA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of PTPLA elisa kit
PTPLA elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS914022. Click to request current manual
NCBI/Uniprot data below describe general gene information for PTPLA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
32,388 Da
NCBI Official Full Name
very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 1
NCBI Official Synonym Full Names
protein tyrosine phosphatase-like (proline instead of catalytic arginine), member A
NCBI Official Symbol
PTPLA  [Similar Products]
NCBI Official Synonym Symbols
CAP; HACD1
  [Similar Products]
NCBI Protein Information
very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 1; very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 1; cementum attachment protein; protein-tyrosine phosphatase-like member A; very-long-chain (3R)-3-hydroxyacyl-[acyl-carrier protein] dehydratase 1
UniProt Protein Name
Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 1
UniProt Synonym Protein Names
3-hydroxyacyl-CoA dehydratase 1; HACD1; Cementum attachment protein; Protein-tyrosine phosphatase-like member A
UniProt Gene Name
PTPLA  [Similar Products]
UniProt Synonym Gene Names
HACD1; HACD1  [Similar Products]
UniProt Entry Name
HACD1_HUMAN
NCBI Summary for PTPLA
The protein encoded by this gene contains a characteristic catalytic motif of the protein tyrosine phosphatases (PTPs) family. The PTP motif of this protein has the highly conserved arginine residue replaced by a proline residue; thus it may represent a distinct class of PTPs. Members of the PTP family are known to be signaling molecules that regulate a variety of cellular processes. This gene was preferentially expressed in both adult and fetal heart. A much lower expression level was detected in skeletal and smooth muscle tissues, and no expression was observed in other tissues. The tissue specific expression in the developing and adult heart suggests a role in regulating cardiac development and differentiation. [provided by RefSeq, Jul 2008]
UniProt Comments for PTPLA
PTPLA: Responsible for the dehydration step in very long-chain fatty acids (VLCFAs) synthesis. Belongs to the very long-chain fatty acids dehydratase HACD family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; EC 4.2.1.134; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10p12.33

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane

Molecular Function: enzyme binding; hydroxyapatite binding; lyase activity; 3-hydroxyacyl-CoA dehydratase activity

Biological Process: sphingolipid biosynthetic process; multicellular organismal development; regulation of G2/M transition of mitotic cell cycle; very-long-chain fatty acid biosynthetic process; myotube differentiation; fatty acid elongation; fatty acid biosynthetic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000