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PTPN1 recombinant protein :: Protein Tyrosine Phosphatase 1B Recombinant Protein

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Catalog # MBS636074
Unit / Price
  0.25 mg  /  $445 +1 FREE 8GB USB
SDS-PAGE
Product Name

Protein Tyrosine Phosphatase 1B (PTPN1), Recombinant Protein

Popular Item
Also Known As

Protein Tyrosine Phosphatase 1B, Recombinant, Human (PTP1B)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
MEMEKEFEQ I/ DKSGSWAAIY / QDIRHEASDF / PCRVAKLPKN / KNRNRYRDVS / PFDHSRIKLH / QEDNDYIN AS/ LIKMEEAQRS / YILTQGPLPN / TCGHFWEMVW / EQKSRGVVML / NRVMEKGSLK / CAQYWPQK EE/ KEMIFEDTNL / KLTLISEDIK / SYYTVRQLEL / ENLTTQETRE / ILHFHYTTWP / DFGVPESP AS/ FLNFLFKVRE / SGSLSPEHGP / VVVHCSAGIG / RSGTFCLADT / CLLLMDKRKD / PSSVDIKK VL/ LEMRKFRMGL / IQTADQLRFS / YLAVIEGAKF / IMGDSSVQDQ / WKELSHEDLE / PPPEHIPP PP/ RPPKRILEPH / N
Chromosome Location
Chromosome: 20; NC_000020.10 (49126858..49201299). Location: 20q13.1-q13.2
OMIM
176885
3D Structure
ModBase 3D Structure for P18031
Host
E Coli
Purity/Purification
Highly Purified
>95% by SDS PAGE; Purified by using conventional chromatography techniques.
Form/Format
Supplied as a liquid in 25mM Tris-HCl, pH 7.5, 2mM beta-mercaptoethanol, 1mM EDTA, 1mM DTT, 20% glycerol.
Concentration
1mg/ml (lot specific)
Specific Activity
>10,000units/mg
Unit Definition
One unit will hydrolyze 1nanomole of p-nitrophenylphosphatate (p-NPP) per minute at pH 7.4, 37 degree C using 10mM of substrate.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C . Aliquots are stable for 6 months at -20 degree C . For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of PTPN1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PTPN1 recombinant protein
The protein coding region of the catalytic domain of PTP-1B (amino acids 1-321) was cloned into an E. coli expression vector.

SDS-PAGE analysis of PTP 1 B (3ug protein; 12% gel)
PTPN1 recombinant protein SDS-PAGE image
NCBI/Uniprot data below describe general gene information for PTPN1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
NCBI Official Full Name
protein tyrosine phosphatase 1B
NCBI Official Synonym Full Names
protein tyrosine phosphatase, non-receptor type 1
NCBI Official Symbol
PTPN1  [Similar Products]
NCBI Official Synonym Symbols
PTP1B
  [Similar Products]
NCBI Protein Information
tyrosine-protein phosphatase non-receptor type 1; protein-tyrosine phosphatase 1B; protein tyrosine phosphatase, placental
UniProt Protein Name
Tyrosine-protein phosphatase non-receptor type 1
UniProt Synonym Protein Names
Protein-tyrosine phosphatase 1B
UniProt Gene Name
PTPN1  [Similar Products]
UniProt Synonym Gene Names
PTP1B; PTP-1B  [Similar Products]
UniProt Entry Name
PTN1_HUMAN
NCBI Summary for PTPN1
The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013]
UniProt Comments for PTPN1
PTP1B: a non-receptor phospho-tyrosine protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation and inactivation of PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. Negatively regulates insulin signaling by dephosphorylating the phosphotyrosine residues of insulin receptor. Also reported to dephosphorylate integrin, epidermal growth factor receptor, JAK2 and TYK2, regulating cell growth control and the cellular response to interferon. May regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET. PTP1B knockout mice show resistance to dietary weight gain and enhanced insulin sensitivity, suggesting a possible role in treatment of obesity as well as type 2 diabetes.

Protein type: Motility/polarity/chemotaxis; Phosphatase; EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor)

Chromosomal Location of Human Ortholog: 20q13.1-q13.2

Cellular Component: endoplasmic reticulum; early endosome; plasma membrane; cytoplasmic vesicle; cytosol

Molecular Function: protein binding; enzyme binding; ephrin receptor binding; zinc ion binding; protein tyrosine phosphatase activity; receptor tyrosine kinase binding; protein kinase binding; insulin receptor binding

Biological Process: platelet activation; unfolded protein response, activation of signaling protein activity; negative regulation of vascular endothelial growth factor receptor signaling pathway; unfolded protein response; cytokine and chemokine mediated signaling pathway; regulation of endocytosis; actin cytoskeleton reorganization; insulin receptor signaling pathway; negative regulation of insulin receptor signaling pathway; regulation of signal transduction; blood coagulation

Disease: Diabetes Mellitus, Noninsulin-dependent
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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