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PTPRN elisa kit :: Human Protein Tyrosine Phosphatase Receptor Type N ELISA Kit

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Catalog # MBS731293
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve
Product Name

Protein Tyrosine Phosphatase Receptor Type N (PTPRN), ELISA Kit

Popular Item
Also Known As

Human Protein Tyrosine Phosphatase Receptor Type N ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
601773
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of PTPRN. No significant cross-reactivity or interference between PTPRN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PTPRN and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
2.5-50ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PTPRN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPTPRNpurchase
MBS731293 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Protein Tyrosine Phosphatase Receptor Type N (PTPRN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PTPRN. The ELISA analytical biochemical technique of the MBS731293 kit is based on PTPRN antibody-PTPRN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PTPRN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PTPRN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PTPRN elisa kit
Intended Uses: This PTPRN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PTPRN. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||PTPRN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PTPRN antibody and an PTPRN-HRP conjugate. The assay sample and buffer are incubated together with PTPRN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PTPRN concentration since PTPRN from samples and PTPRN-HRP conjugate compete for the anti-PTPRN antibody binding site. Since the number of sites is limited, as more sites are occupied by PTPRN from the sample, fewer sites are left to bind PTPRN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTPRN concentration in each sample is interpolated from this standard curve.
Product Categories/Family for PTPRN elisa kit

Typical Testing Data/Standard Curve of PTPRN elisa kit
PTPRN elisa kit Typical Testing Data/Standard Curve image
Sample Manual Insert of MBS731293. Click to request current manual
NCBI/Uniprot data below describe general gene information for PTPRN. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
96,261 Da
NCBI Official Full Name
PTPRN protein, partial
NCBI Official Synonym Full Names
protein tyrosine phosphatase, receptor type, N
NCBI Official Symbol
PTPRN  [Similar Products]
NCBI Official Synonym Symbols
IA2; IA-2; ICA512; R-PTP-N; IA-2/PTP
  [Similar Products]
NCBI Protein Information
receptor-type tyrosine-protein phosphatase-like N; ICA 512; PTP IA-2; islet cell antigen 2; islet cell antigen 512; islet cell autoantigen 3; protein tyrosine phosphatase-like N
UniProt Protein Name
Receptor-type tyrosine-protein phosphatase-like N
UniProt Synonym Protein Names
Islet cell antigen 512; ICA 512; Islet cell autoantigen 3; PTP IA-2
UniProt Gene Name
PTPRN  [Similar Products]
UniProt Synonym Gene Names
ICA3; ICA512; R-PTP-N; ICA 512  [Similar Products]
UniProt Entry Name
PTPRN_HUMAN
NCBI Summary for PTPRN
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single catalytic domain, and thus represents a receptor-type PTP. This PTP was found to be an autoantigen that is reactive with insulin-dependent diabetes mellitus (IDDM) patient sera, and thus may be a potential target of autoimmunity in diabetes mellitus. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Dec 2010]
UniProt Comments for PTPRN
PTPRN: Implicated in neuroendocrine secretory processes. May be involved in processes specific for neurosecretory granules, such as their biogenesis, trafficking or regulated exocytosis or may have a general role in neuroendocrine functions. Seems to lack intrinsic enzyme activity. May play a role in the regulation of secretory granules via its interaction with SNTB2. Belongs to the protein-tyrosine phosphatase family. Receptor class 8 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Receptor protein phosphatase, tyrosine

Chromosomal Location of Human Ortholog: 2q35-q36.1

Cellular Component: integral to membrane

Molecular Function: protein binding; GTPase binding; spectrin binding; protein tyrosine phosphatase activity

Biological Process: response to reactive oxygen species; response to cAMP; response to estrogen stimulus; insulin secretion; cytokine and chemokine mediated signaling pathway; response to glucose stimulus; protein amino acid dephosphorylation; response to insulin stimulus
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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