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Pycard elisa kit :: Mouse Apoptosis-associated speck-like protein containing a CARD ELISA Kit

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Catalog # MBS2885479
Unit / Price
  48-Strip-Wells  /  $355 +1 FREE 8GB USB
  96-Strip-Wells  /  $475 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,030 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,700 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Apoptosis-associated speck-like protein containing a CARD (Pycard), ELISA Kit

Popular Item
Also Known As

Mouse Apoptosis-associated speck-like protein containing a CARD ELISA Kit

Product Synonym Names
Apoptosis-associated speck-like protein containing a CARD; mASC; PYD and CARD domain-containing protein; Pycard; Asc
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS2885479 Testing Data
Sequence Length
3D Structure
ModBase 3D Structure for Q9EPB4
Species Reactivity
Recombinant and natural Mouse Apoptosis-associated speck-like protein containing a CARD,mASC
Assay Type
Detection Range
78-5000 pg/mL
40.2 pg/mL
Intra-Assay CV
Inter-Assay CV
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Pycard elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPycardpurchase
MBS2885479 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Apoptosis-associated speck-like protein containing a CARD (Pycard) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Pycard. The ELISA analytical biochemical technique of the MBS2885479 kit is based on Pycard antibody-Pycard antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Pycard antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Pycard. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
Pycard elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of Pycard elisa kit
Pycard elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2885479. Click to request current manual
NCBI/Uniprot data below describe general gene information for Pycard. It may not necessarily be applicable to this product.
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
21,459 Da
NCBI Official Full Name
apoptosis-associated speck-like protein containing a CARD
NCBI Official Synonym Full Names
PYD and CARD domain containing
NCBI Official Symbol
Pycard  [Similar Products]
NCBI Official Synonym Symbols
Asc; TNS1; masc; CARD5; TMS-1; 9130417A21Rik
  [Similar Products]
NCBI Protein Information
apoptosis-associated speck-like protein containing a CARD
UniProt Protein Name
Apoptosis-associated speck-like protein containing a CARD
UniProt Synonym Protein Names
PYD and CARD domain-containing protein
UniProt Gene Name
Pycard  [Similar Products]
UniProt Synonym Gene Names
Asc; mASC  [Similar Products]
UniProt Entry Name
UniProt Comments for Pycard
PYCARD: Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. Forms complexes with other DAPIN domain-containing proteins. Interacts with CIAS1/PYPAF1 and PYDC1. Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U-937, but not in Jurkat T- cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and MOLT-4 lymphocytic leukemia cells. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; Adaptor/scaffold

Cellular Component: cell soma; cytoplasm; cytosol; endoplasmic reticulum; extracellular region; IkappaB kinase complex; mitochondrion; nucleolus; nucleus; protein complex

Molecular Function: apoptotic protease activator activity; enzyme binding; identical protein binding; interleukin-6 receptor binding; myosin I binding; protease binding; protein binding; protein dimerization activity; protein homodimerization activity; Pyrin domain binding; tropomyosin binding

Biological Process: activation of innate immune response; activation of NF-kappaB transcription factor; apoptosis; caspase activation; defense response to Gram-negative bacterium; defense response to virus; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; immune system process; inflammatory response; inhibition of NF-kappaB transcription factor; innate immune response; interleukin-1 beta production; myeloid dendritic cell activation; myeloid dendritic cell activation during immune response; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of interferon-beta production; positive regulation of actin filament polymerization; positive regulation of activated T cell proliferation; positive regulation of adaptive immune response; positive regulation of antigen processing and presentation of peptide antigen via MHC class II; positive regulation of apoptosis; positive regulation of caspase activity; positive regulation of defense response to virus by host; positive regulation of interferon-gamma production; positive regulation of interleukin-1 beta secretion; positive regulation of interleukin-6 production; positive regulation of JNK cascade; positive regulation of phagocytosis; positive regulation of T cell activation; positive regulation of transcription factor activity; positive regulation of tumor necrosis factor production; regulation of apoptosis; regulation of autophagy; regulation of caspase activity; regulation of GTPase activity; regulation of inflammatory response; regulation of protein stability; response to bacterium; tumor necrosis factor-mediated signaling pathway
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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