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PYCARD protein :: Apoptosis-associated speck-like protein containing a CARD Protein

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Catalog # MBS2889532
Unit / Price
  0.1 mg  /  $525 +1 FREE 8GB USB
  0.2 mg  /  $795 +1 FREE 8GB USB
  0.5 mg  /  $1,365 +1 FREE 8GB USB
  1 mg  /  $1,455 +1 FREE 8GB USB
Testing Data(TD)
Product Name

Apoptosis-associated speck-like protein containing a CARD (PYCARD), Protein

Popular Item
Also Known As

Apoptosis-associated speck-like protein containing a CARD

Product Synonym Names
ASC; CARD5; TMS1; Caspase recruitment domain-containing protein 5; PYD and CARD domain-containing protein; Target of methylation-induced silencing 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
1-195aa
Sequence Length
195
Sequence
MGRARDAILD ALENLTAEEL KKFKLKLLSV PLREGYGRIP RGALLSMDAL DLTDKLVSFY LETYGAELTA NVLRDMGLQE MAGQLQAATH QGSGAAPAGI QAPPQSAAKP GLHFIDQHRA ALIARVTNVE WLLDALYYGK VLTDEQYQAV RAEPTNPSKM RKLFSFTPAW NWTTCKDLLL QALRESQSYL VEDLERS
OMIM
606838
3D Structure
ModBase 3D Structure for Q9ULZ3
Host
E Coli
Purity/Purification
>90%
Form/Format
Liquid
Concentration
1 mg/mL (lot specific)
Organism
Homo sapiens(human)
Buffer
50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10%glycerol(PH8.0)
Preparation and Storage
Store at -20°C for 6 months.
Avoid repeated freezing and thawing.
Other Notes
Small volumes of PYCARD protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PYCARD protein
Recombinant protein with His-tag.
Applications Tested/Suitable for PYCARD protein
ELISA (EIA), Western Blot (WB), Affinity Purification (AP)

Testing Data(TD) of PYCARD protein
PYCARD protein Testing Data(TD) image
NCBI/Uniprot data below describe general gene information for PYCARD. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
21.45 kD
NCBI Official Full Name
apoptosis-associated speck-like protein containing a CARD isoform a
NCBI Official Synonym Full Names
PYD and CARD domain containing
NCBI Official Symbol
PYCARD  [Similar Products]
NCBI Official Synonym Symbols
ASC; TMS; TMS1; CARD5; TMS-1
  [Similar Products]
NCBI Protein Information
apoptosis-associated speck-like protein containing a CARD
UniProt Protein Name
Apoptosis-associated speck-like protein containing a CARD
UniProt Synonym Protein Names
Caspase recruitment domain-containing protein 5; PYD and CARD domain-containing protein; Target of methylation-induced silencing 1
UniProt Gene Name
PYCARD  [Similar Products]
UniProt Synonym Gene Names
ASC; CARD5; TMS1; hASC  [Similar Products]
NCBI Summary for PYCARD
This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for PYCARD
Functions as key mediator in apoptosis and inflammation. Promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a probable cell type-specific manner. Involved in activation of the mitochondrial apoptotic pathway, promotes caspase-8-dependent proteolytic maturation of BID independently of FADD in certain cell types and also mediates mitochondrial translocation of BAX and activates BAX-dependent apoptosis coupled to activation of caspase-9, -2 and -3. Involved in macrophage pyroptosis, a caspase-1-dependent inflammatory form of cell death and is the major constituent of the ASC pyroptosome which forms upon potassium depletion and rapidly recruits and activates caspase-1. In innate immune response believed to act as an integral adapter in the assembly of the inflammasome which activates caspase-1 leading to processing and secretion of proinflammatory cytokines. The function as activating adapter in different types of inflammasomes is mediated by the pyrin and CARD domains and their homotypic interactions. Required for recruitment of caspase-1 to inflammasomes containing certain pattern recognition receptors, such as NLRP2, NLRP3, AIM2 and probably IFI16. In the NLRP1 and NLRC4 inflammasomes seems not be required but facilitates the processing of procaspase-1. In cooperation with NOD2 involved in an inflammasome activated by bacterial muramyl dipeptide leading to caspase-1 activation. May be involved in DDX58-triggered proinflammatory responses and inflammasome activation. Isoform 2 may have a regulating effect on the function as inflammasome adapter. Isoform 3 seems to inhibit inflammasome-mediated maturation of interleukin-1 beta. In collaboration with AIM2 which detects cytosolic double-stranded DNA may also be involved in a caspase-1-independent cell death that involves caspase-8. In adaptive immunity may be involved in maturation of dendritic cells to stimulate T-cell immunity and in cytoskeletal rearrangements coupled to chemotaxis and antigen uptake may be involved in post-transcriptional regulation of the guanine nucleotide exchange factor DOCK2; the latter function is proposed to involve the nuclear form. Also involved in transcriptional activation of cytokines and chemokines independent of the inflammasome; this function may involve AP-1, NF-kappa-B, MAPK and caspase-8 signaling pathways. For regulation of NF-kappa-B activating and inhibiting functions have been reported. Modulates NF-kappa-B induction at the level of the IKK complex by inhibiting kinase activity of CHUK and IKBK. Proposed to compete with RIPK2 for association with CASP1 thereby down-regulating CASP1-mediated RIPK2-dependent NF-kappa-B activation and activating interleukin-1 beta processing. Modulates host resistance to DNA virus infection, probably by inducing the cleavage of and inactivating CGAS in presence of cytoplasmic double-stranded DNA (PubMed:28314590).
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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