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PYGM recombinant protein :: Glycogen Phosphorylase, Muscle (PYGM) Recombinant Protein

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Catalog # MBS2011599 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
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  0.01 mg  /  $150 +1 FREE 8GB USB
  0.05 mg  /  $295 +1 FREE 8GB USB
  0.1 mg  /  $465 +1 FREE 8GB USB
  0.2 mg  /  $580 +1 FREE 8GB USB
  0.5 mg  /  $1,120 +1 FREE 8GB USB
  1 mg  /  $1,665 +2 FREE 8GB USB
Product Name

Glycogen Phosphorylase, Muscle (PYGM), Recombinant Protein

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Full Product Name

Recombinant Glycogen Phosphorylase, Muscle (PYGM)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF-RKQISV RGLA GVENVSELKK NFNRHLHFTL VKDRNVATPR DYYFALAHTV RDHLVGRWIR TQQHYYEKDP KRIYYLSLEF YMGRTLQNTM VNLALENACD EATYQLGLDM EELEEIEEDA GLGNGGLGRL AACFLDSMAT LGLAAYGYGI RYEFGIFNQK ICGGWQMEEA DDWLRYG
Chromosome Location
Chromosome: 19; NC_000085.6 (6384429..6398459). Location: 19 A; 19 4.53 cM
3D Structure
ModBase 3D Structure for Q9WUB3
Host
E Coli
Purity/Purification
>98%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
Organism
Mus musculus (Mouse)
Expression System
Prokaryotic expression
Residues
Arg11~Gly187 with N-terminal His-Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Predicted isoelectric point
6.0
Predicted Molecular Mass
21.7kDa
Accurate Molecular Mass
26kDa as determined by SDS-PAGE reducing conditions.
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
Tissue Specificity
Skeletal Muscle, Brain, Heart, Kidney.
Traits
Freeze-dried powder
Note
The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affect the charge of the protein.
3. Post-translational modification:Phosphorylation, glycosylation, methylation etc.
4. Post-translation clevage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of PYGM recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for PYGM recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), CoIP, ReporterAssays, Purification, Amine Reactive Labeling.

Sequence Information of PYGM recombinant protein
PYGM recombinant protein Sequence Information image
Testing Data of PYGM recombinant protein
PYGM recombinant protein Testing Data image
NCBI/Uniprot data below describe general gene information for PYGM. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
Predicted Molecular Weight: 22.0kDa
Accurate Molecular Mass: 26kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
glycogen phosphorylase, muscle form
NCBI Official Synonym Full Names
muscle glycogen phosphorylase
NCBI Official Symbol
NCBI Official Synonym Symbols
PG; AI115133
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NCBI Protein Information
glycogen phosphorylase, muscle form; myophosphorylase
UniProt Protein Name
Glycogen phosphorylase, muscle form
UniProt Synonym Protein Names
Myophosphorylase
Protein Family
UniProt Gene Name
UniProt Entry Name
PYGM_MOUSE
UniProt Comments for PYGM
PYGM: an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Dimers associate into a tetramer to form the enzymatically active phosphorylase A. Phosphorylation of Ser-14 converts phosphorylase B (unphosphorylated) to phosphorylase A.

Protein type: Phosphorylase; Transferase; EC 2.4.1.1; Carbohydrate Metabolism - starch and sucrose; Endoplasmic reticulum

Cellular Component: sarcoplasmic reticulum; cytoplasm; Z disc

Molecular Function: transferase activity; glycogen phosphorylase activity; transferase activity, transferring glycosyl groups; phosphorylase activity; nucleotide binding; drug binding; carbohydrate binding; catalytic activity; pyridoxal phosphate binding; AMP binding

Biological Process: cellular calcium ion homeostasis; glycogen metabolic process; response to organic substance; glycogen catabolic process; response to cAMP; metabolic process; carbohydrate metabolic process; response to hypoxia
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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