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RABGEF1 elisa kit :: Human RAB guanine nucleotide exchange factor (GEF) 1 ELISA Kit

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Catalog # MBS9325962
Unit / Price
  48-Strip-Wells  /  $470 +1 FREE 8GB USB
  96-Strip-Wells  /  $680 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
RABGEF1 elisa kit
Product Name

RAB guanine nucleotide exchange factor (GEF) 1 (RABGEF1), ELISA Kit

Popular Item
Full Product Name

Human Rab5 GDP/GTP exchange factor, RABGEF1 ELISA Kit

Product Synonym Names
Human Rab5 GDP/GTP exchange factor (RABGEF1) ELISA kit; FLJ32302; RABEX5; rabex-5; OTTHUMP00000210543; Rab5 GDP/GTP exchange factor; RAB guanine nucleotide exchange factor (GEF) 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
609700
3D Structure
ModBase 3D Structure for Q9UJ41
Species Reactivity
Specificity
No significant cross-reactivity or interference between this analyte and analogues is observed.
Assay Type
Sandwich
Detection Range
0.25 ng/ml - 8 ng/ml.
Sensitivity
0.1 ng/ml.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
Preparation and Storage
Store all reagents at 2-8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of RABGEF1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forRABGEF1purchase
MBS9325962 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the RAB guanine nucleotide exchange factor (GEF) 1 (RABGEF1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RABGEF1. The ELISA analytical biochemical technique of the MBS9325962 kit is based on RABGEF1 antibody-RABGEF1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RABGEF1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RABGEF1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
RABGEF1 elisa kit
Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of RABGEF1 (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
Sample Manual Insert of MBS9325962. Click to request current manual
NCBI/Uniprot data below describe general gene information for RABGEF1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
79,371 Da
NCBI Official Full Name
rab5 GDP/GTP exchange factor isoform a
NCBI Official Synonym Full Names
RAB guanine nucleotide exchange factor (GEF) 1
NCBI Official Symbol
RABGEF1  [Similar Products]
NCBI Official Synonym Symbols
RAP1; RABEX5; rabex-5
  [Similar Products]
NCBI Protein Information
rab5 GDP/GTP exchange factor; rabaptin-5-associated exchange factor for Rab5
UniProt Protein Name
Rab5 GDP/GTP exchange factor
UniProt Synonym Protein Names
RAP1; Rabaptin-5-associated exchange factor for Rab5; Rabex-5
UniProt Gene Name
RABGEF1  [Similar Products]
UniProt Synonym Gene Names
RABEX5  [Similar Products]
UniProt Entry Name
RABX5_HUMAN
NCBI Summary for RABGEF1
RABGEF1 forms a complex with rabaptin-5 (RABPT5; MIM 603616) that is required for endocytic membrane fusion, and it serves as a specific guanine nucleotide exchange factor (GEF) for RAB5 (RAB5A; MIM 179512) (Horiuchi et al., 1997 [PubMed 9323142]).[supplied by OMIM, Mar 2010]
UniProt Comments for RABGEF1
RABGEF1: Rab effector protein acting as linker between gamma- adaptin, RAB4A or RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates nucleotide exchange on RAB5A. Can act as a ubiquitin ligase. Interacts with RGS14; the interaction is GTP-dependent. Heterodimer with RABEP1. The heterodimer binds RAB4A and RAB5A that have been activated by GTP-binding. Interacts with RAB21, and with 100-fold lower affinity also with RAB22. Binds TSC2, GGA1, GGA2, GGA3, AP1G1 and AP1G2. Interacts with ubiquitinated EGFR. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; GAPs, Rab

Chromosomal Location of Human Ortholog: 7q11.21

Cellular Component: recycling endosome; early endosome; vesicle

Molecular Function: protein binding; DNA binding; zinc ion binding; ubiquitin-protein ligase activity; Rab guanyl-nucleotide exchange factor activity; Rab GTPase binding

Biological Process: negative regulation of protein amino acid phosphorylation; negative regulation of mast cell degranulation; negative regulation of inflammatory response; protein ubiquitination; negative regulation of Ras protein signal transduction; negative regulation of receptor-mediated endocytosis; endocytosis; protein targeting to membrane; negative regulation of leukocyte migration
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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