• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Rag2 recombinant protein :: V (D)J recombination-activating protein 2 Recombinant Protein

Scan QR to view Datasheet
Catalog # MBS1001277
Unit / Price
Scan QR to view Datasheet
  0.05 mg (E-Coli)  /  $950 +1 FREE 8GB USB
  0.05 mg (Yeast)  /  $1,000 +1 FREE 8GB USB
  0.05 mg (Baculovirus)  /  $1,235 +1 FREE 8GB USB
  0.2 mg (E-Coli)  /  $1,275 +1 FREE 8GB USB
  0.2 mg (Yeast)  /  $1,360 +1 FREE 8GB USB
  0.5 mg (E-Coli)  /  $1,405 +1 FREE 8GB USB
  0.05 mg (Mammalian-Cell)  /  $1,475 +1 FREE 8GB USB
  0.5 mg (Yeast)  /  $1,545 +1 FREE 8GB USB
  0.1 mg (Baculovirus)  /  $1,790 +2 FREE 8GB USB
  1 mg (E-Coli)  /  $2,130 +2 FREE 8GB USB
  0.5 mg (Baculovirus)  /  $2,315 +2 FREE 8GB USB
  0.1 mg (Mammalian-Cell)  /  $2,385 +2 FREE 8GB USB
  1 mg (Yeast)  /  $2,450 +3 FREE 8GB USB
  1 mg (Baculovirus)  /  $3,570 +4 FREE 8GB USB
Rag2 recombinant protein
Product Name

V (D)J recombination-activating protein 2 (Rag2), Recombinant Protein

Full Product Name

Recombinant Mouse V (D)J recombination-activating protein 2

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
1-527aa, full length protein
Sequence
MSLQMVTVGH NIALIQPGFS LMNFDGQVFF FGQKGWPKRS CPTGVFHFDI KQNHLKLKPA IFSKDSCYLP PLRYPATCSY KGSIDSDKHQ YIIHGGKTPN NELSDKIYIM SVACKNNKKV TFRCTEKDLV GDVPEPRYGH SIDVVYSRGK SMGVLFGGRS YMPSTQRTTE KWNSVADCLP HVFLIDFEFG CATSYILPEL QDGLSFHVSI ARNDTVYILG GHSLASNIRP ANLYRIRVDL PLGTPAVNCT VLPGGISVSS AILTQTNNDE FVIVGGYQLE NQKRMVCSLV SLGDNTIEIS EMETPDWTSD IKHSKIWFGS NMGNGTIFLG IPGDNKQAMS EAFYFYTLRC SEEDLSEDQK IVSNSQTSTE DPGDSTPFED SEEFCFSAEA TSFDGDDEFD TYNEDDEDDE SVTGYWITCC PTCDVDINTW VPFYSTELNK PAMIYCSHGD GHWVHAQCMD LEERTLIHLS EGSNKYYCNE HVQIARALQT PKRNPPLQKP PMKSLHKKGS GKVLTPAKKS FLRRLFD
3D Structure
ModBase 3D Structure for P21784
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>85% (SDS-PAGE) (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Species
Mouse
Storage Buffer
Tris-based buffer, 50% glycerol
Tag Information
Tag type will be determined during the manufacturing process
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of Rag2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
Rag2 recombinant protein
This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms.
NCBI/Uniprot data below describe general gene information for Rag2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
59,074 Da
NCBI Official Full Name
V(D)J recombination-activating protein 2
NCBI Official Synonym Full Names
recombination activating gene 2
NCBI Official Symbol
NCBI Official Synonym Symbols
Rag-2
  [Similar Products]
NCBI Protein Information
V(D)J recombination-activating protein 2
UniProt Protein Name
V(D)J recombination-activating protein 2
UniProt Gene Name
UniProt Synonym Gene Names
Rag-2; RAG-2  [Similar Products]
UniProt Comments for Rag2
Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with Rag2 recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Thymus Antibodies  >285 publications with Rag2 and Thymus
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000