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RANK active protein :: RANK-Ligand Active Protein

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Catalog # MBS506102
Unit / Price
Scan QR to view Datasheet
  0.002 mg  /  $145 +1 FREE 8GB USB
  0.01 mg  /  $235 +1 FREE 8GB USB
  0.1 mg  /  $715 +1 FREE 8GB USB
  0.5 mg  /  $3,310 +4 FREE 8GB USB
  1 mg  /  $5,440 +6 FREE 8GB USB
Product Name

RANK-Ligand, Active Protein

Popular Item
Full Product Name

Animal-free Human RANK-Ligand

Product Synonym Names
Animal Free Recombinant Human soluble RANK Ligand; soluble RANK Ligand; TNFSF11; TRANCE; OPGL; ODF
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
EKAMVDGSW LDLAKRSKLE AQPFAHLTIN ATDIPSGSHK VSLSSWYHDR GWAKISNMTF SNGKLIVNQD GFYYLYANIC FRHHETSGDL ATEYLQLMVY VTKTSIKIPS SHTLMKGGST KYWSGNSEFH FYSINVGGFF KLRSGEEISI EVSNPSLLDP DQDATYFGAF KVRDID
OMIM
AF019047 mRNA
3D Structure
ModBase 3D Structure for O14788
Host
E Coli
Purity/Purification
Purity is typically 95% as determined by reducing and non-reducing SDS-PAGE
Form/Format
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing
10 mM sodium phosphate, pH 7.5
Species
Human
Bioactivity Assay
bioactive protein but no acceptaince criteria limits established
Reconstitution
Sterile water at 0.1 mg/mL
Acceptance Criteria LAL (EUs/ug)
<=1.00
Monomer/Dimer
Monomer
Endotoxin Level (kinetic LAL analysis)
Typically less than 0.05 EUs/mug protein (50% confidence range)
Biological Activity
The activity is determined by a dose-dependent stimulation of IL-8 production in human PBMC and is typically less than 100 ng/mL. This corresponds to an expected specific activity of 1 x 104 units/mg.
Animal Components
This product is produced with no animal-derived raw products. All processing and handling employs animal-free equipment and animal-free protocols.
Media Components
Luria Broth
Preparation and Storage
12 months from date of receipt when stored at -20 degree C to-80 degree C as supplied.
1 month when stored at 4 degree C after reconstituting as directed.
3 months when stored at -20 degree C to -80 degree C after reconstituting as directed.
Active Protein Note
Refer to the datasheet for activity details. For other activity applications, researchers should evaluate this protein for suitability in their model system.
Other Notes
Small volumes of RANK active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
RANK active protein
Receptor activator of nuclear factor kappa-B Ligand (RANKL) is a cell-bound marker related to the tumor necrosis factor (TNF) family of proteins. RANKL plays a critical role in bone metabolism and osteoclast differentiation. T cell expression of RANKL promotes dendritic cell maturation.

RANK active protein Gel Data image
Biological Activity of RANK active protein
RANK active protein Biological Activity image
Testing Data of RANK active protein
RANK active protein Testing Data image
NCBI/Uniprot data below describe general gene information for RANK. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Non-glycosylated protein, containing 176 amino acids, with a molecular weight of 20 kDa.
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 11 isoform 1
NCBI Official Synonym Full Names
tumor necrosis factor superfamily member 11
NCBI Official Symbol
TNFSF11  [Similar Products]
NCBI Official Synonym Symbols
ODF; OPGL; sOdf; CD254; OPTB2; RANKL; TNLG6B; TRANCE; hRANKL2
  [Similar Products]
NCBI Protein Information
tumor necrosis factor ligand superfamily member 11
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 11
UniProt Synonym Protein Names
Osteoclast differentiation factor; ODF
UniProt Gene Name
TNFSF11  [Similar Products]
UniProt Synonym Gene Names
OPGL; RANKL; TRANCE; ODF; OPGL; RANKL; TRANCE  [Similar Products]
NCBI Summary for RANK
This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq, Jul 2008]
UniProt Comments for RANK
TNFSF11: Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Homotrimer. Up-regulated by T-cell receptor stimulation. Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid. Belongs to the tumor necrosis factor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q14.11

Cellular Component: extracellular region; plasma membrane

Molecular Function: cytokine activity; tumor necrosis factor receptor superfamily binding

Biological Process: activation of NF-kappaB transcription factor; calcium-mediated signaling; cytokine and chemokine mediated signaling pathway; monocyte chemotaxis; osteoclast differentiation; positive regulation of bone resorption; positive regulation of corticotropin-releasing hormone secretion; positive regulation of homotypic cell-cell adhesion; positive regulation of MAP kinase activity; positive regulation of osteoclast differentiation; positive regulation of T cell activation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; tumor necrosis factor-mediated signaling pathway

Disease: Osteopetrosis, Autosomal Recessive 2
Research Articles on RANK
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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