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RANKL recombinant protein :: RANKL, soluble Recombinant Protein

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Catalog # MBS691818
Unit / Price
  0.002 mg  /  $200 +1 FREE 8GB USB
  0.01 mg  /  $340 +1 FREE 8GB USB
RANKL recombinant protein
Product Name

RANKL, soluble, Recombinant Protein

Popular Item
Also Known As

Rat RANKL, soluble

Product Synonym Names
Soluble Receptor Activator of NFkB Ligand; TNFSF11; TRANCE (TNF-related activation-induced cytokine); OPGL; ODF (Osteoclast differentiation factor)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
174
Sequence
PAMMEGSWLD VARRGKPEAQ PFAHLTINAA DIPSGSHKVS LSSWYH DRGW AKISNMTLSN GKLRVNQDGF YYLYANICFR HHETSGSVPA D YLQLMVYVV KTSIKIPSSH NLMKGGSTKN WSGNSEFHFY SINVGGF FKL RAGEEISVQV SNPSLLDPDQ DATYFGAFKV QDID
Chromosome Location
Chromosome: 15; NC_005114.3 (64155680..64186116, complement). Location: 15q11
3D Structure
ModBase 3D Structure for Q9ESE2
Host
E Coli
Species Reactivity
Rat
Purity/Purification
> 98% by SDS-PAGE & HPLC analyses
Form/Format
Lyophilized
Endotoxin level
<0.1 ng /ug of protein (<1EU/ug)
Reconsitution
Centrifuge the vial prior to opening.Reconstitute in water to a concentration of 0.1-1.0 mg/m. do not vertex. This solution can be stored at 2-8 degree C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 degree C to -80 degree C.
Biological Activity
Determined by its ability to induce NFkappaB in RAW264.7 cells in the absence of any cross-linking. The expected ED50 for this effect is 10.0-25.0 ng/ml.
Preparation and Storage
Stability: The lyophilized protein is stable at room temperature for 1 month and at 4 degree C for 6 months. Reconstituted working aliquots are stable for 1 week at 2 degree C to 8 degree C and for 3 months at -20 degree C to -80 degree C.
Avoid freeze/thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of RANKL recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
RANKL recombinant protein
RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic-cell-membrane protein, which by interacting with RANKL augments the ability of dendritic cells to stimulate naive T-cell proliferation in a mixed lymphocyte reaction, to promote the survival of RANK + T cells, and to regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. Recombinant rat sRANKL is a 19.4 kDa polypeptide comprising the TNF homologous region of RANKL (174 amino acid residues).
NCBI/Uniprot data below describe general gene information for RANKL. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
19.4kDa
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 11
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 11
NCBI Official Symbol
Tnfsf11  [Similar Products]
NCBI Official Synonym Symbols
RANKL
  [Similar Products]
NCBI Protein Information
tumor necrosis factor ligand superfamily member 11; ODF; OPGL; TRANCE; osteoprotegerin ligand; osteoclast differentiation factor; TNF-related activation-induced cytokine; receptor activator of nuclear factor kappa B ligand; receptor activator of nuclear factor kappa-B ligand
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 11
UniProt Synonym Protein Names
Osteoclast differentiation factor; ODF; Osteoprotegerin ligand; OPGL; Receptor activator of nuclear factor kappa-B ligand; RANKL; TNF-related activation-induced cytokine
UniProt Gene Name
Tnfsf11  [Similar Products]
UniProt Synonym Gene Names
Opgl; Rankl; Trance; ODF; OPGL; RANKL; TRANCE  [Similar Products]
UniProt Entry Name
TNF11_RAT
NCBI Summary for RANKL
induces osteoclast formation and bone resoprtion; plays a role in regulation of calcium homeostasis and osteoclast function [RGD, Feb 2006]
UniProt Comments for RANKL
Function: Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.

Subunit structure: Homotrimer. Interacts with TNFRSF11A and TNFRSF11B

By similarity.

Subcellular location: Cell membrane; Single-pass type II membrane protein

By similarity. Tumor necrosis factor ligand superfamily member 11, soluble form: Secreted

By similarity.

Tissue specificity: Highly expressed in thymus and bone tissues.

Post-translational modification: The soluble form derives from the membrane form by proteolytic processing

By similarity.

Sequence similarities: Belongs to the tumor necrosis factor family.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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