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RBP elisa kit :: Canine Retinol Binding Protein ELISA Kit

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Catalog # MBS740925
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Retinol Binding Protein (RBP), ELISA Kit

Popular Item
Also Known As

Canine Retinol Binding Protein ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene 615233
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
25-500mg/L
Sensitivity
1.0 mg/L
Intended Uses
This RBP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine RBP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of RBP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forRBPpurchase
MBS740925 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Retinol Binding Protein (RBP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RBP. The ELISA analytical biochemical technique of the MBS740925 kit is based on RBP antibody-RBP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RBP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RBP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
RBP elisa kit
Principle of the Assay: RBP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RBP antibody and an RBP-HRP conjugate. The assay sample and buffer are incubated together with RBP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RBP concentration since RBP from samples and RBP-HRP conjugate compete for the anti-RBP antibody binding site. Since the number of sites is limited, as more sites are occupied by RBP from the sample, fewer sites are left to bind RBP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RBP concentration in each sample is interpolated from this standard curve.
Product Categories/Family for RBP elisa kit

Typical Testing Data/Standard Curve (for reference only) of RBP elisa kit
RBP elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS740925. Click to request current manual
NCBI/Uniprot data below describe general gene information for RBP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
135,363 Da
NCBI Official Full Name
retinol-binding protein
NCBI Official Synonym Full Names
retinol binding protein 3, interstitial
NCBI Official Symbol
NCBI Official Synonym Symbols
IRBP; RBPI; RP66; D10S64; D10S65; D10S66
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NCBI Protein Information
retinol-binding protein 3; interphotoreceptor retinoid-binding protein
UniProt Protein Name
Retinol-binding protein 3
UniProt Synonym Protein Names
Interphotoreceptor retinoid-binding protein; IRBP; Interstitial retinol-binding protein
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
RET3_HUMAN
NCBI Summary for RBP
Interphotoreceptor retinol-binding protein is a large glycoprotein known to bind retinoids and found primarily in the interphotoreceptor matrix of the retina between the retinal pigment epithelium and the photoreceptor cells. It is thought to transport retinoids between the retinal pigment epithelium and the photoreceptors, a critical role in the visual process.The human IRBP gene is approximately 9.5 kbp in length and consists of four exons separated by three introns. The introns are 1.6-1.9 kbp long. The gene is transcribed by photoreceptor and retinoblastoma cells into an approximately 4.3-kilobase mRNA that is translated and processed into a glycosylated protein of 135,000 Da. The amino acid sequence of human IRBP can be divided into four contiguous homology domains with 33-38% identity, suggesting a series of gene duplication events. In the gene, the boundaries of these domains are not defined by exon-intron junctions, as might have been expected. The first three homology domains and part of the fourth are all encoded by the first large exon, which is 3,180 base pairs long. The remainder of the fourth domain is encoded in the last three exons, which are 191, 143, and approximately 740 base pairs long, respectively. [provided by RefSeq, Jul 2008]
UniProt Comments for RBP
RBP3: IRBP shuttles 11-cis and all trans retinoids between the retinol isomerase in the pigment epithelium and the visual pigments in the photoreceptor cells of the retina. Belongs to the peptidase S41A family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: extracellular space; extracellular region; vesicle

Molecular Function: retinoid binding; serine-type peptidase activity; retinol binding; retinal binding

Biological Process: phototransduction, visible light; visual perception; transport; lipid metabolic process; retinoid metabolic process; proteolysis

Disease: Retinitis Pigmentosa 66; Retinitis Pigmentosa
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with RBP elisa kit
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