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RBP4 elisa kit :: Guinea Pig RBP4 ELISA Kit

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Catalog # MBS2504534
Unit / Price
  48-Strip-Wells  /  $450 +1 FREE 8GB USB
  96-Strip-Wells  /  $535 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,255 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,975 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

RBP4, ELISA Kit

Popular Item
Also Known As

Guinea pig RBP4 (Retinol Binding Protein 4, Plasma) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
201
OMIM
180250
3D Structure
ModBase 3D Structure for P02753
Species Reactivity
Specificity
This kit recognizes natural and recombinant Guinea pig RBP4. No significant cross-reactivity or interference between Guinea pig RBP4 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
3.125-200ng/mL
Sensitivity
Min: 1.875ng/mL; Max: 200ng/mL
Intended Uses
This ELISA kit applies to the in vitro quantitative determination of Guinea pig RBP4 concentrations in serum, plasma and other biological fluids.
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of RBP4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forRBP4purchase
MBS2504534 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the RBP4, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RBP4. The ELISA analytical biochemical technique of the MBS2504534 kit is based on RBP4 antibody-RBP4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RBP4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RBP4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
RBP4 elisa kit
Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to RBP4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for RBP4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain RBP4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of RBP4. You can calculate the concentration of RBP4 in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of RBP4 elisa kit
RBP4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2504534. Click to request current manual
NCBI/Uniprot data below describe general gene information for RBP4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
23,010 Da
NCBI Official Full Name
retinol-binding protein 4
NCBI Official Synonym Full Names
retinol binding protein 4, plasma
NCBI Official Symbol
NCBI Official Synonym Symbols
RDCCAS
  [Similar Products]
NCBI Protein Information
retinol-binding protein 4; PRBP; plasma retinol-binding protein; retinol-binding protein 4, interstitial
UniProt Protein Name
Retinol-binding protein 4
UniProt Synonym Protein Names
Plasma retinol-binding protein; PRBP; RBP
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
PRBP; RBP  [Similar Products]
UniProt Entry Name
RET4_HUMAN
NCBI Summary for RBP4
This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. [provided by RefSeq, Jul 2008]
UniProt Comments for RBP4
RBP4: Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli. Defects in RBP4 are a cause of retinol-binding protein deficiency (RBP deficiency). This condition causes night vision problems. It produces a typical 'fundus xerophthalmicus', featuring a progressed atrophy of the retinal pigment epithelium. Belongs to the calycin superfamily. Lipocalin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q23.33

Cellular Component: extracellular space; protein complex; extracellular region; cytosol

Molecular Function: protein binding; protein heterodimerization activity; retinal binding; retinol binding

Biological Process: cardiac muscle development; phototransduction, visible light; response to retinoic acid; retinal metabolic process; urinary bladder development; maintenance of gastrointestinal epithelium; embryonic skeletal development; heart development; male gonad development; positive regulation of insulin secretion; vagina development; positive regulation of immunoglobulin secretion; uterus development; glucose homeostasis; gluconeogenesis; response to insulin stimulus; embryonic organ morphogenesis; response to ethanol; eye development; negative regulation of cardiac muscle cell proliferation; retinol metabolic process; embryonic retina morphogenesis in camera-type eye; detection of light stimulus involved in visual perception; spermatogenesis; retinoid metabolic process; female genitalia morphogenesis; lung development

Disease: Retinal Dystrophy, Iris Coloboma, And Comedogenic Acne Syndrome; Microphthalmia, Isolated, With Coloboma 10
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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