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RC3H1 sirna

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Catalog # MBS8240255
Unit / Price
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  15 nmol  /  $365 +1 FREE 8GB USB
  30 nmol  /  $515 +1 FREE 8GB USB
RC3H1 sirna
Product Name

RC3H1, siRNA

Popular Item
Full Product Name

RC3H1 siRNA (Human)

Product Synonym Names
KIAA2025; RNF198; Roquin-1; Roquin; RING finger and C3H zinc finger protein 1; RING finger and CCCH-type zinc finger domain-containing protein 1; RING finger protein 198
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
609424
3D Structure
ModBase 3D Structure for Q5TC82
Host
Synthetic
Species Reactivity
Human
Specificity
RC3H1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RC3H1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of RC3H1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
RC3H1 sirna
siRNA to inhibit RC3H1 expression using RNA interference
Applications Tested/Suitable for RC3H1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for RC3H1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
124,842 Da
NCBI Official Full Name
roquin-1 isoform a
NCBI Official Synonym Full Names
ring finger and CCCH-type domains 1
NCBI Official Symbol
RC3H1  [Similar Products]
NCBI Official Synonym Symbols
RNF198; ROQUIN
  [Similar Products]
NCBI Protein Information
roquin-1
UniProt Protein Name
Roquin-1
UniProt Synonym Protein Names
RING finger and C3H zinc finger protein 1; RING finger and CCCH-type zinc finger domain-containing protein 1; RING finger protein 198
Protein Family
UniProt Gene Name
RC3H1  [Similar Products]
UniProt Synonym Gene Names
KIAA2025; RNF198; Roquin  [Similar Products]
UniProt Entry Name
RC3H1_HUMAN
NCBI Summary for RC3H1
This gene encodes a protein containing RING-type and C3H1-type zinc finger motifs. The encoded protein recognizes and binds to a constitutive decay element (CDE) in the 3' UTR of mRNAs, leading to mRNA deadenylation and degradation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
UniProt Comments for RC3H1
RC3H1: Represses ICOS function, an essential costimulatory receptor for follicular T-cells. May act as an ubiquitin ligase that regulates ubiquitin-dependent control of AU-rich mRNAs by AUF1/HNRPD and HUR/ELAVL1. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; RNA-binding

Chromosomal Location of Human Ortholog: 1q25.1

Cellular Component: stress granule

Molecular Function: mRNA 3'-UTR binding; zinc ion binding

Biological Process: T cell proliferation; spleen development; negative regulation of germinal center formation; negative regulation of T-helper cell differentiation; B cell homeostasis; negative regulation of activated T cell proliferation; regulation of T cell receptor signaling pathway; protein ubiquitination; T cell homeostasis; cytoplasmic mRNA processing body assembly; negative regulation of B cell proliferation; regulation of mRNA stability; lymph node development; regulation of germinal center formation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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