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Whole Leukocyte Esterase Reagent

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Catalog # MBS173116
Unit / Price
  1 mL  /  $180 +1 FREE 8GB USB
  5 mL  /  $380 +1 FREE 8GB USB
  25 mL  /  $1,140 +1 FREE 8GB USB
Whole Leukocyte Esterase reagent
Product Name

Whole Leukocyte Esterase, Reagent

Popular Item
Also Known As

Leukocyte Esterase, Human Leukocytes

Product Synonym Names
White Blood Cells; WBC; Whole Leukocytes
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS173116 Technical Datasheet
Form/Format
Liquid suspension in 154mM sodium chloride
Source
Human Leukocytes
Unit Definition
One unit will catalyze the hydrolysis of one micromole of methoxy succinyl-alanine-proline-valine-p-nitroanilide per minute at 37°C and pH 7.5 (In the presence of 500 mM sodium chloride and 0.2% Triton X-100)
Note
On lysing the leukocytes, 0.45 mL per liter of saline will give a positive reaction for leukocytes with Roche Chemstrip 10 SG or Siemens Multistix 10 SG strips.
Activity
5.2 U/mL
Grade
Control grade
Donors
Donor material originates from TSE, CJD, and vCJD-free countries.
Appearance
Milky white to off-white suspension
Odor
None
Handling
Handle as a potentially hazardous susbstance.
Recovery
Centrifuge briefly to ensure complete recovery
Preparation and Storage
Store at -20°C.
Avoid freeze/thaw cycles.
Shipping: Ships with dry ice.
Other Notes
Small volumes of Whole Leukocyte Esterase reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
Whole Leukocyte Esterase reagent
MyBioSource is the leading supplier of Human Leukocyte Esterase for use in urine test kits and manufacturing of urine controls by major IVD manufacturers around the world.

On lysing the leukocytes, 0.45 mL per liter of saline will give a positive reaction for leukocytes with Roche Chemstrip 10 with SG or Siemens Multistix 10 SG strips.

Technical support, bulk quantities and aliquoting available. Single lot sizes from 5 to 50 L.

Leukocyte Esterase is used in a urine test for presence of white blood cells abnormalities associated with infection. Urine tests reveal the presence of granulocyte esterases. The esterases cleave a derivatized pyrazole amino acid ester to liberate derivatized hydroxy pyrazole. This pyrazole then reacts with a diazonium salt to produce a purple color.

White blood cells in the urine usually indicate a urinary tract infection. Human leukocytes esterase test detects human esterase, an enzyme released by white blood cells. Human White blood cells are produced in the bone marrow and help to defend the body against infectious disease and foreign materials as part of the immune system. Human Leukocytosis is very common in acutely ill patients. It occurs in response to a wide variety of conditions, including viral, bacterial, fungal, or parasitic infection, cancer, and exposure to certain medications or chemicals.

Human leukocytes are used in routine dipstick tests and urine controls. If this Human leukocyte test is positive, the urine should be examined microscopically for the presence of white blood cells and other abnormalities associated with infection.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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