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Bacterial outer membrane protein A Recombinant Protein

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Catalog # MBS400001
Unit / Price
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  0.05 mg  /  $190 +1 FREE 8GB USB
  0.1 mg  /  $255 +1 FREE 8GB USB
  0.5 mg  /  $785 +1 FREE 8GB USB
  1 mg  /  $1,230 +1 FREE 8GB USB
  5 mg  /  $4,070 +5 FREE 8GB USB
  10 mg  /  $6,645 +8 FREE 8GB USB
Bacterial outer membrane protein A recombinant protein
Product Name

Bacterial outer membrane protein A, Recombinant Protein

Popular Item
Full Product Name

Bacterial outer membrane protein A

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
The N-terminal amino sequence is Met-Asp-Va l-Val-Ile- Ser
Purity/Purification
Greater than 98.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
(c) Gel-filtration chromatography under non denaturing conditions.
Form/Format
White lyophilized (freeze-dried) powder.
Source
E.coli
Solubility
It is recommended to reconstitute the lyophilized A-protein in sterile 0.4% NaHCO3 adjusted, not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Protein Content
Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.58 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Dimers and Aggregates
Less than 3%
Endotoxin
Less than 0.1 ng/ug (IEU/ug) of A-protein
Formulation
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02% NaHCO3.
Biological Activity
The interaction of bacterial and recombinant A-layer protein with murine macrophages was directed at determining the effect of A-protein on intracellular events that occur in primed macrophages. This was accomplished by measuring the cytotoxic product produced by peritoneal macrophages when exposed to A-protein coated latex beads. Thioglycolate elicited macrophages exhibited a low level of activation (18% cytotoxicity) that was significantly increased (48% cytotoxicity) in the presence of latex beads. Coating of the latex beads with each of the three A-protein products resulted in an increase of cytoxicity (mean +/- SEM) from 48% to 91%.
Preparation and Storage
Lyophilized A-protein although stable at room temperature for several weeks, should be stored desiccated below -18 degree C. Upon reconstitution at > 0.1 A-protein mg/ml and up to 2 mg and filter sterilization A-protein can be stored at +4 degree C
Other Notes
Small volumes of Bacterial outer membrane protein A recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Achromogenic atypical Aeromonas salmonicida is the causative agent of goldfish ulcer disease. Virulence of this bacterium is associated with the production of a paracrystalline outer membrane A-layer protein. The species specific structural gene for the monomeric form of A-protein was cloned into a pET-3d plasmid in order to express and produce a recombinant form of the protein in E. coli BL21(DE3). The induced protein was isolated from inclusion bodies by a simple solubilization-renaturation procedure and purified by ion exchange chromatography on Q-Sepharose to over 95% pure monomeric protein. Recombinant A-protein was compared by biochemical, immunological and molecular methods with the A-protein isolated from atypical A. salmonicida bacterial cells by the glycine and the membrane extraction methods. The recombinant form was found to be undistinguishable from the wild type when examined by SDS-PAGE and gel filtration chromatography yielding a 50.5 kDa monomeric protein with isoelectric point of 5.35. The immunological similarity of the protein samples was demonstrated by employing polyclonal and monoclonal antibodies in ELISA and Western Blot techniques. All forms of A-protein were found to activate the secretion of tumour necrosis factor alpha from murine macrophage. For reference see Maurice et al. (1999) Protein Expression and Purification 16, 396-404.  
NCBI/Uniprot data below describe general gene information for Bacterial outer membrane protein A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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