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Hepatitis A Virus, P3C Recombinant Protein

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Catalog # MBS637723
Unit / Price
  0.1 mg  /  $410 +1 FREE 8GB USB
  0.5 mg  /  $835 +1 FREE 8GB USB
Hepatitis A Virus, P3C recombinant protein
Product Name

Hepatitis A Virus, P3C, Recombinant Protein

Popular Item
Full Product Name

Hepatitis A Virus, P3C, Recombinant (HAV)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Host
E Coli
Specificity
Immunoreactive with sera of HAV-infected individuals
Purity/Purification
>90% by 10 PAGE with coomassie staining. Chromatographically purifies.
Form/Format
Supplied as a liquid in 10mM CBB, pH 9.6, 0.1% SDS, 50% glycerol.
Concentration
1mg/ml (lot specific)
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of Hepatitis A Virus, P3C recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
Hepatitis A Virus, P3C recombinant protein
Forty-two antigenic domains were identified across the hepatitis A virus (HAV) polyprotein by using a set of 237 overlapping 20-mer synthetic peptides spanning the entire HAV polyprotein and a panel of serum samples from acutely HAV-infected patients. The term "antigenic domain" is used in this study to define a protein region spanned with consecutive overlapping immunoreactive peptides. Nineteen antigenic domains were found within the structural proteins, and 22 were found within the nonstructural proteins, with 1 domain spanning the junction of VP1 and P2A proteins. Five of these domains were considered immunodominant, as judged by both the breadth and the strength of their immunoreactivity. One domain is located within the VP2 protein at position 57-90 aa. A second domain, located at position 767-842 aa, contains the C-terminal part of the VP1 protein and the entire P2A protein. A third domain, located at position 1403-1456 aa, comprises the C-terminal part of the P2C protein and the N-terminal half of the P3A protein. The fourth domain, located at position 1500-1519 aa, includes almost the entire P3B, and the last domain, located at position 1719-1764 aa, contains the C-terminal region of the P3C protein and the N-terminal region of the P3D protein. It is interesting to note that four of the five most immunoreactive domains are derived from small HAV proteins and/or encompass protein cleavage sites separating different HAV proteins.
Recombinant HAV Antigen may be used in ELISA and Western Blot, excellent for detection of HAV with minimal specificity problems.

Note: Recombinant protein corresponding to aa1643-1743 containing the P3C immunodominant regions from the Hepatitis A Virus expressed in E.coli.
Molecular Weight
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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