View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List Leptin Triple Antagonist Active Protein Catalog # MBS142229 Unit / Price 0.01 mg / $245 +1 FREE 8GB USB 0.05 mg / $320 +1 FREE 8GB USB 1 mg / $1,855 +2 FREE 8GB USB 5x1 mg / $8,005 +10 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Leptin Triple Antagonist, Active Protein Popular Item Full Product Name Recombinant Human Leptin Triple Antagonist Product Synonym Names Leptin tA Human; Leptin Antagonist Triple Mutant Human Recombinant; Leptin tA Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sequence The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pr o-Ile-Gln Host E Coli Purity/Purification Greater than 98.0% as determined by: (a) Gel filtration analysis. (b) Analysis by SDS-PAGE. Form/Format The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.White lyophilized (freeze-dried) powder. Solubility It is recommended to reconstitute the lyophilized Leptin Antagonist Triple Mutant Human Recombinant in sterile 0.4% NaHCO3adjusted to pH 8-9, not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.88 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). Biological Activity MyBioSource's Leptin triple antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. It also inhibits various leptin effects in several in vitro bioassays. Preparation and Storage Lyophilized Leptin Antagonist Triple Mutant Human Recombinant although stable at room temperature for several weeks, should be stored desiccated below -18 degree C. Upon reconstitution at > 0.1 mg/ml and up to 2 mM and filter sterilization Leptin mutant can be stored at 4 degree C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles. Active Protein Note Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product. Other Notes Small volumes of Leptin Triple Antagonist active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for Leptin Triple Antagonist active protein Description: Leptin Antagonist Triple Mutant Human Recombinant is a single non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular weight of 16 kDa, Leptin was mutated, resulting in L39A/D40A/F41A. Leptin Antagonist Triple Mutant Human Recombinant was purified by proprietary chromatographic techniques. Product Categories/Family for Leptin Triple Antagonist active protein Cytokines and Growth Factors; Growth Factors; Leptin Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP