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REG3G elisa kit :: Bovine Regenerating islet-derived protein 3-gamma ELISA Kit

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Catalog # MBS755934
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Regenerating islet-derived protein 3-gamma (REG3G), ELISA Kit

Popular Item
Also Known As

Bovine Regenerating islet-derived protein 3-gamma ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
609933
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of REG-3?. No significant cross-reactivity or interference between REG-3? and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between REG-3? and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
1.0-25ng/mL
Sensitivity
0.1ng/mL
Intended Uses
This REG-3? ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine REG-3?. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of REG3G elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forREG3Gpurchase
MBS755934 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Regenerating islet-derived protein 3-gamma (REG3G) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing REG3G. The ELISA analytical biochemical technique of the MBS755934 kit is based on REG3G antibody-REG3G antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect REG3G antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, REG3G. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
REG3G elisa kit
Principle of the assay: REG-3? ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-REG-3? antibody and an REG-3?-HRP conjugate. The assay sample and buffer are incubated together with REG-3?-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the REG-3? concentration since REG-3? from samples and REG-3?-HRP conjugate compete for the anti-REG-3? antibody binding site. Since the number of sites is limited, as more sites are occupied by REG-3? from the sample, fewer sites are left to bind REG-3?-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The REG-3? concentration in each sample is interpolated from this standard curve.
Product Categories/Family for REG3G elisa kit

Typical Testing Data/Standard Curve (for reference only) of REG3G elisa kit
REG3G elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS755934. Click to request current manual
NCBI/Uniprot data below describe general gene information for REG3G. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
14,409 Da
NCBI Official Full Name
regenerating islet-derived protein 3-gamma isoform 1
NCBI Official Synonym Full Names
regenerating islet-derived 3 gamma
NCBI Official Symbol
REG3G  [Similar Products]
NCBI Official Synonym Symbols
PAP1B; PAPIB; PAP IB; PAP-1B; UNQ429; LPPM429; REG III; REG-III
  [Similar Products]
NCBI Protein Information
regenerating islet-derived protein 3-gamma; REG-3-gamma; reg III-gamma; regenerating gene III; pancreatitis-associated protein 1B; pancreatitis-associated protein IB; regenerating islet-derived protein III-gamma
UniProt Protein Name
Regenerating islet-derived protein 3-gamma
UniProt Synonym Protein Names
Pancreatitis-associated protein 1B; PAP-1B; Pancreatitis-associated protein IB; PAP IB; Regenerating islet-derived protein III-gamma; REG III; Reg III-gamma
UniProt Gene Name
REG3G  [Similar Products]
UniProt Synonym Gene Names
PAP1B; UNQ429/PRO162; REG-3-gamma; PAP-1B; PAP IB; REG III; Reg III-gamma  [Similar Products]
UniProt Entry Name
REG3G_HUMAN
NCBI Summary for REG3G
This gene encodes a member of the regenerating islet-derived genes (REG)3 protein family. These proteins are secreted, C-type lectins with a carbohydrate recognition domain and N-terminal signal peptide. The protein encoded by this gene is an antibacterial lectin with activity against Gram-positive bacteria. Alternative splicing results in multiple transcript variants encoding multiple isoforms. [provided by RefSeq, Jun 2012]
UniProt Comments for REG3G
REG3G: Might be a stress protein involved in the control of bacterial proliferation.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2p12

Cellular Component: cytoplasm; extracellular region

Molecular Function: carbohydrate binding

Biological Process: MyD88-dependent toll-like receptor signaling pathway; defense response to Gram-positive bacterium; negative regulation of keratinocyte differentiation; acute-phase response
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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